[1]却志群.草莓斑驳病毒的RT-PCR技术检测[J].北方园艺,2012,36(07):132-134.
　QUE Zhi-qun.Detection of Strawberry Mottle Virus with RT-PCR[J].Northern Horticulture,2012,36(07):132-134.
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草莓斑驳病毒的RT-PCR技术检测

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年07期 页码: 132-134 栏目: 出版日期: 2012-07-16
Title:
Detection of Strawberry Mottle Virus with RT-PCR
文章编号:
1001-0009（2012）07-0132-03
作者:
却志群
宜春学院 生命科学与资源环境学院,江西 宜春　336000
Author(s):
QUE Zhi-qun
College of Life Sciences and Resources Environment Sciences,Yichun University,Yichun，Jiangxi 336000
关键词:
草莓斑驳病毒; 引物; CTAB; RNA; RT-PCR; 特异片段
Keywords:
strawberry mottle virus; primer; CTAB; RNA; RT-PCR; specific fragment
分类号:
S 436.639
文献标志码:
A
摘要:
针对草莓斑驳病毒(SMV)基因组外壳蛋白保守序列设计特异引物，利用改进的CTAB法提取出优质的草莓斑驳病毒的总RNA，然后进行 RT-PCR分子检测以期建立草莓斑驳病毒的RT-PCR检测体系。结果表明：RNA质量完好，证明改进的CTAB法应用于草莓总RNA的提取是切实可行的。
Abstract:
Special primers were design based on the sequence of coat protein which was a conservative region of strawberry mottle virus.Highly pure total RNA including strawberry and strawberry mottle virus was extracted with modified CTAB method.Reverse transcription-polymerase chain reaction (RT-PCR ) was carried out in order to establish detection system of strawberry mottle virus.The results showed that a good RNA quality was obtained and modified CTAB method was feasible for extraction of total RNA of strawberry.

参考文献/References:

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[1]李颖,谢占玲,田飞,等.黄绿蜜环菌SSR-PCR反应体系的优化及引物筛选[J].北方园艺,2013,37(16):114.
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备注/Memo

作者简介：却志群（1980-）,女,湖北仙桃人,硕士,讲师,研究方向为植物组织培养与分子植物病理学。E-mail：zhiqunque@163.com。

基金项目：江西省宜春学院院级资助项目。
收稿日期：2011-12-19

更新日期/Last Update: 2014-09-03