ZHANG Dan,KANG Yong-xiang,XUE Li-yan,et al.Study on the Influencing Factors of Initial Culture of Cornus walteri Wanger.[J].Northern Horticulture,2012,36(07):123-125.
毛梾初代培养影响因素研究
- Title:
- Study on the Influencing Factors of Initial Culture of Cornus walteri Wanger.
- 文章编号:
- 1001-0009（2012）07-0123-03
- Keywords:
- Cornus walteri Wanger.; initial culture; influencing factors
- 分类号:
- S 792.119
- 文献标志码:
- A
- 摘要:
- 以毛梾茎段为外植体，研究了不同消毒方式、不同基本培养基类型、不同取材时间和不同激素组合对毛梾初代培养的影响。结果表明：0.1%氯化汞10 min取得了比较理想的消毒效果,污染率和褐化率均较低；MS培养基为较适宜的初代培养基，其萌发时间、生长状况均优于其它培养基；外植体在MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+IBA 0.1 mg/L上生长最好。
- Abstract:
- Taking the stems of Cornus walteri Wanger. as explants,the disinfection methods，basic medium，different sampling time and different hormone affect the primary culture of Cornus walteri Wanger.were studied.The results showed that the best disinfection method was using 0.1%HgCl2 for 10 minutes，it had lower contaminated rate and browning rate；MS medium was the best one for primary culture，the germination time and growth conditions were better than other medium；explants grew well in the medium of MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+IBA 0.1 mg/L.
参考文献/References:
[1]陈有民.园林树木学[M].北京:中国林业出版社,2006:616-617.
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备注/Memo
第一作者简介：张丹（1986-），女，山西运城人，在读硕士，现主要从事组织培养方面的研究工作。