LUO Cheng-hua,YAN Jie,ZHU Jian-bo.Establish of High Frequency Regeneration System of Taraxacum kok-saghyz Rodin[J].Northern Horticulture,2012,36(07):115-119.
橡胶草高频再生体系的建立
- Title:
- Establish of High Frequency Regeneration System of Taraxacum kok-saghyz Rodin
- 文章编号:
- 1001-0009（2012）07-0115-05
- Keywords:
- Taraxacum kok-saghyz Rodin; regeneration system; rooting
- 分类号:
- S 682.1+9
- 文献标志码:
- A
- 摘要:
- 以橡胶草叶片为外植体，研究了消毒时间、外源激素等因素对外植体成活率、不定芽的诱导、伸长和生根的影响。结果表明：用0.1%的升汞处理7 min为最佳消毒方法，最佳的再生培养基为MS+1.5 mg/L 6-BA+0.1 mg/L NAA，平均诱导率为97.3%，芽伸长培养基为MS+2.0 mg/L 6-BA+0.02 mg/L NAA+0.4 mg/L GA3，生根培养基为1/2MS+0.2 mg/L NAA，生根率为100%，所获得的组培苗生长健壮且移栽成活率高，最终摸索出了橡胶草最佳的再生体系。
- Abstract:
- The effects of disinfection time and exogenous hormones on the survival rate，induction of in vitro shoot，elongation and root formation were studied by taking young leaf of Taraxacum kok-saghyz Rodin as explants.The results showed that the optimum disinfection method was treated with 0.1% HgCl2 for 7 min；the optimum regeneration medium was MS+1.5 mg/L 6-BA+0.1 mg/L NAA and the average induction rate was 97.3%；the optimum shoot regeneration medium was MS+2.0 mg/L 6-BA+0.02 mg/L NAA+0.4 mg/L GA3；the best rooting medium was 1/2MS+0.2 mg/L NAA，the rooting rate was 100%，the tissue cultured plants obtained grew well and had high survival rate after transplanting，the optimum regeneration system of Taraxacum kok-saghyz Rodin was found finally.
参考文献/References:
[1]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1999:45-46.
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备注/Memo
第一作者简介：罗成华（1989-），男，湖北公安人，在读硕士，现主要从事植物基因工程研究工作。E-mail:luochenghua890420@163.com。