XU Ling-fei,LI Zhi-hui,JIA Dong-feng,et al.Study on in vitro Propagation of Yunnan Quince as a Dwarf Rootstock of Pear[J].Northern Horticulture,2012,36(05):113-115.
梨矮化砧木云南榅桲离体快繁研究
- Title:
- Study on in vitro Propagation of Yunnan Quince as a Dwarf Rootstock of Pear
- 文章编号:
- 1001-0009（2012）05-0113-03
- Keywords:
- pear; dwarf rootstock; Yunnan quince; in vitro propagation
- 分类号:
- S 661.204+.3
- 文献标志码:
- A
- 摘要:
- 以梨矮化砧木云南榅桲的茎段为外植体,进行了离体快繁研究。结果表明：茎芽增殖的适宜培养基为MS+6-BA 1.0 mg/L+IBA 0.3 mg/L或MS+6-BA 0.5 mg/L+NAA 0.5 mg/L,增殖系数分别为3.65和3.58，并且试管苗长势健壮；云南榅桲不定根诱导的适宜培养基为1/2MS+NAA 0.3 mg/L,生根苗移栽成活率为92%。
- Abstract:
- The stem segments with axillary buds of Yunnan quince were used as the explants to develop in vitropropagation protocol.The results indicated that the optimal shoot multiplication medium was MS+6-BA 1.0 mg/L+IBA 0.3 mg/L or MS+6-BA 0.5 mg/L+NAA 0.5 mg/L，the multiplication rate was 3.65 and 3.58，and the new shoot was strong.The suitable rooting medium was 1/2MS+NAA 0.3 mg/L.The survival percentage was 92% when rooting plantlets were transplanted in soil.
参考文献/References:
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[2]徐凌飞,尚晓峰,邓丰产,等.梨异属矮化砧密植栽培模式要点[M]//张绍铃.梨产业技术研究与应用.北京：中国农业出版社,2010:315-317.
[3]徐明义,姚芳玲.矮化中间砧梨简介[J].农业科技与信息,1996(10):12.
[4]D’Onofrio C,Morini S.Somatic embryo，adventitious root and shoot regeneration in in vitro grown quince leaves as influenced by treatments of different length with growth regulators [J].Scientia Horticulturae,2006,107:194-199.
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备注/Memo
第一作者简介： 徐凌飞(1969-)，男，博士，副教授，现主要从事梨树种质资源和生物技术改良研究工作。E-mail: lingfxu@yahoo.com。
基金项目：现代农业产业技术体系建设专项资金资助项目（CARS-29-40）。收稿日期：2011-12-02