[1]王洪霞,郭尚敬.TDZ对甜椒不定芽分化的影响[J].北方园艺,2012,36(03):104-106.
　WANG Hong-xia,GUO Shang-jing.Effect of TDZ on Adventitious Bud Differentiation of Sweet Pepper[J].Northern Horticulture,2012,36(03):104-106.
点击复制

TDZ对甜椒不定芽分化的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年03期 页码: 104-106 栏目: 出版日期: 2012-03-16
Title:
Effect of TDZ on Adventitious Bud Differentiation of Sweet Pepper
文章编号:
1001-0009（2012）03-0104-03
作者:
王洪霞; 郭尚敬
聊城大学 生命科学学院，山东 聊城 252059
Author(s):
WANG Hong-xia; GUO Shang-jing
College of Life Science，Liaocheng University，Liaocheng,Shandong 252059
关键词:
甜椒; 组织培养; TDZ
Keywords:
Capsicum; plant regeneration; TDZ
分类号:
S 641.303.6
文献标志码:
A
摘要:
为了建立甜椒高频、高效离体培养再生体系,以甜椒子叶、真叶、下胚轴为外植体,采用了不同浓度的TDZ 与IAA、6-BA的配比,研究了TDZ对甜椒不定芽分化的影响。结果表明：仅含有TDZ的培养基中芽分化率几乎为0,仅含有IAA、6-BA的培养基中芽分化率最高达80%,加入TDZ后,在0.5 mg/L IAA+10.0 mg/L 6-BA+0.5 mg/L TDZ的激素组合培养条件下,可获得96%的丛生芽诱导频率。表明在甜椒芽诱导过程中添加TDZ能够很好地提高分化效率。
Abstract:
High-frequency and high-efficiency in vitro culture regeneration system of sweet pepper were established,cotyledon,leaf,hycopotys were used as explants and different concentrations of IAA,6-BA,TDZ,the effect of TDZ on adventitious bud differentiation of sweet pepper were discussed.The results showed that the bud differentiation rate was almost zero in the medium only containing TDZ;the highest bud differentiation rate was 80% in the medium only containing IAA and 6-BA;however the induction frequency was about 96% in the medium containing 0.5 mg/L IAA+10.0 mg/L 6-BA+0.5 mg/L TDZ,TDZ could improve differentiation efficiency in pepper bud induction process.

参考文献/References:

[1]Gunay A L In vitro plant regeneration from hypocotyland cotyledon explants of red pepper ( Capsicum annuum ?L.) [J].Plant Sci Lett,1978(11):365-372.

[2]王玉文,杨美珠,潘乃燧.甜椒的离体再生及基因转化[J].植物学报,1991,33(10)：780-786.
[3]曹冬孙,贾士荣.青椒子叶培养及植株再生[J].园艺学报,1993,20(2)：171-175.
[4]黎定军,张宝玺,赵开军,等.辣椒子叶高效植株再生体系建立[J].园艺学报,2002,29(1):25-29.
[5]徐晓峰,黄学林.TDZ:一种有效的植物生长调节剂[J].植物学报,2003,20(2):227-237.
[6]Mok M C,Mok D W S,Armstrong D J.Cytokinin activity of N-pheny 1-N-1,2,3-thiadiazol-5yl urea and its effect on cytokinin autonomy in callus cultures of Phasealus[J].Plant Physical，1980,65：24.[7]Huetteman C A,Preece J E.Thidiazuron:a potent cytokinin for woody plant tissue culture[J].Plant Cell,Tissue and Organ Culture,1993,33:105-119.
[8]胥宇建,孟箭,张鲁刚,等.不同激素配比和AgNO3对两种基因型大白菜子叶离体再生的影响[J].植物生理学通讯,2010,46(6):564-570.
[9]陈雄伟,陈刚,王瑛华.TDZ和CPPU对鼎湖山紫背天葵不定芽诱导的影响[J].安徽农业科学,2009,37(27):13336-13338.
[10]Fellman C D,Read P E,Hosier M A.Effect of Thidiazuron and CPPU on meristem formation and shoot proliferation [J].HortSci,1986,22：1197.
[11]Thomas J C,Katterman F R.Cytokinin activity induced by thidiazuron [J].Plant Physiol,1986,81:681-683.
[12]Suttle J C.Effect of the defoliant thidazuron on ethylene evolution from mung bean hypocotyls segments[J].Plant Physiol,1984,75：902-907.

相似文献/References:

[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
　CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(03):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
　ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(03):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
　WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(03):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
　ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(03):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
　DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(03):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
　ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(03):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
　LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(03):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
　CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(03):138.

备注/Memo

第一作者简介：王洪霞（1981-）,女,山东聊城人,硕士,现主要从事植物分子遗传学研究工作。E-mail:wanghongxiaat2009@163.com。

责任作者：郭尚敬（1970-）,男,山东聊城人,副教授,现主要从事植物分子遗传学研究工作。E-mail:guoshangjing@163.com。
基金项目：山东省优秀中青年科学家基金资助项目（2008BS07008）；国家自然科学基金资助项目（30671242）。
收稿日期：2011-10-25

更新日期/Last Update: 2014-08-26