YANG Dan,GU De-feng,DONG Ran,et al.Study on Rapid Propagation in vitro of Hosta plantaginea of Three New Species[J].Northern Horticulture,2012,36(01):124-126.
三种玉簪新品种离体快繁技术的研究
- Title:
- Study on Rapid Propagation in vitro of Hosta plantaginea of Three New Species
- 文章编号:
- 1001-0009(2012)01-0124-03
- Keywords:
- Hosta plantaginea; adventitious buds; tissue culture
- 分类号:
- S 682.1+9
- 文献标志码:
- A
- 摘要:
- 以3种玉簪“金头饰”(H.‘Gloden Tiaia’)、“小黄金叶”(H.‘Gloden Cadet’)和“金鹰”(H.‘Gloden. Eagle’)的幼嫩花蕾为外植体诱导不定芽，成功的建立了3个品种的离体快繁体系。结果表明：不定芽诱导最佳培养基为MS+6-BA 2.5 mg/L+NAA 0.2 mg/L；在增殖培养基中，“金头饰”最佳增殖培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L；“小黄金叶”最佳增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L；“金鹰”最佳增殖培养基MS+6-BA 4.0 mg/L+NAA 0.2 mg/L；无生长调节剂的MS为“金鹰”的最佳生根培养基,生根率达100%以上;“小黄金叶”在培养基MS +NAA 0.5 mg/L中生根率达到100%以上;“金头饰”在培养基MS +NAA 0.3 mg/L中生根率也达到100%以上。其移栽成活率均为100%。
- Abstract:
- The young buds of H.‘Gloden Tiaia’, H.‘Gloden Gadet’and H.‘Gloden Eagle’were used as the explants to develop a protocol for adventitious buds induced. The successful establishment of five species of rapid propagation system. The results showed that the best medium for adventitious buds induced was MS+6-BA 2.5 mg/L+ NAA 0.2 mg/L; In the proliferation culture, the best proliferation medium for H.‘Gloden Tiaia’was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L; The best proliferation medium for H.‘Gloden Gadet’was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L; The best proliferation medium for H.‘Gloden Eagle’was MS+6-BA 4.0 mg/L+NAA 0.2 mg/L. Medium MS as H.‘Gloden Eagle’rooting medium, the rooting rate was over 100%; H.‘Gloden Gadet’in the rooting medium MS + NAA 0.5 mg/L;rooting rate was over 100%;H.‘Gloden Tiaia’in the rooting medium MS + NAA 0.3 mg/L; rooting rate was over 100%. The transplant survival rate of 100%.
参考文献/References:
[1]余树勋.玉簪花的繁殖[J].中国花卉盆景,2004(5): 8-9.
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备注/Memo
第一作者简介：杨丹（1984-）,女，在读硕士,现主要从事植物组织培养研究工作。E-mail:yangdanmoon@163.com。