[1]尹淑丽.,麻耀华.,张丽萍.,等.不同生防菌对黄瓜根际土壤微生物数量及土壤酶活性的影响[J].北方园艺,2012,36(01):10-14.
　YIN Shu-li.,MA Yao-hua.,ZHANG Li-ping.,et al.Effects of Different Biocontrol Agents on Soil Microbial Number and Enzyme Activity in the Cucumber Rhizosphere[J].Northern Horticulture,2012,36(01):10-14.
点击复制

不同生防菌对黄瓜根际土壤微生物数量及土壤酶活性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年01期 页码: 10-14 栏目: 出版日期: 2012-01-16
Title:
Effects of Different Biocontrol Agents on Soil Microbial Number and Enzyme Activity in the Cucumber Rhizosphere
文章编号:
1001-0009(2012)01-0010-05
作者:
尹淑丽1; 2.; 麻耀华1; 2.; 张丽萍1; 2.; 张根伟1; 2.; 黄亚丽1; 2.; 梁然3
1.河北省科学院 生物研究所，河北 石家庄 050081；
2.河北省主要农作物病害微生物控制工程技术研究中心，河北 石家庄 050081;
3.鹿泉铜冶农业技术推广区域站，河北 石家庄 050200
Author(s):
YIN Shu-li1; 2.;  MA Yao-hua1; 2.;  ZHANG Li-ping1; 2.;  ZHANG Gen-wei1; 2.;  HUANG Ya-li1; 2.;  LIANG Ran3;  ZHOU Jing1; 2.;  CUI Guan-hui1; 2.;  DUAN Pu-fan1; 2.
1.Hebei Academy of Sciences Institute of Biology, Shijiazhuang, Hebei 050081;?
2. Main Crops Disease of Microbial Control Engineering Technology Research Center in Hebei Province, Shijiazhuang, Hebei 050081;?
3.Luquan Tongye Regional Station of Agricultural Technology Extension, Shijiazhuang, Hebei 050200
关键词:
生防菌; 黄瓜根际; 微生物数量; 土壤酶活
Keywords:
biocontrol agents;  cucumber rhizosphere;  soil microbial number;  enzyme activity
分类号:
S 642.206+.1
文献标志码:
A
摘要:
采用盆栽试验的方法，于播种时穴施单一生防菌细菌D、放线菌317和木霉菌，研究黄瓜不同生育时期根际土壤中微生物数量及酶活性的动态变化。结果表明：细菌D和放线菌317的施入利于细菌和放线菌数量的增加，同时有效控制土壤中真菌数量的增加；木霉菌的施入利于土壤中放线菌真菌数量的增加；3个菌株对根际土壤中蔗糖酶、脲酶和过氧化氢酶都有促进的作用，在拉秧期各酶活达到最大值。拉秧期的结果表明，接种细菌D和放线菌317的处理可提高根际土壤中细菌的数量，降低真菌的数量，对放线菌数量的影响不明显；接种木霉处理可明显提高根际土壤中放线菌和真菌的数量，对细菌的数量影响不明显；3个菌株处理细菌与真菌数量比约为对照的1～2倍，放线菌与真菌的数量比约为对照的1～3倍。细菌D、放线菌317和木霉处理均可提高黄瓜根际土壤中过氧化氢酶、蔗糖酶及脲酶的活性。
Abstract:
Pot experiments were carried out to investigate effect of single biocontrol agents(bacteria D, actinomycetes 317, fungi) on soil microbial number and enzyme activity in the different growing stages cucumber rhizosphere. The treatment of bacteria D and actinomycetes 317 could raise the number of bacteria and actinomycetes, decrease the number of fungi; the treatment of fungi could raise the number of actinomycetes and fungi; three agents could raise the activity of Catalase, invertase, urease , the activity was the biggest in the wane period. The results in the wane period showed that the treatments of inoculating D bacteria and 317 actinomyces could increase the number of bacteria and decrease the number of fungi, but could not effect apparently the number of actinomyces; the treatment of inoculating Trichoderma could increase the number of actinomyces and fungi, but could not effect apparently the number of bactira; compared with the control, three treatments of the ratios of bactria and fungi raised 1～2 times, the ratios of actinomyces and fungi were increased apparently. Three different treatments could increase Catalase, invertase, urease activity of cucumber rhizosphere soil.

参考文献/References:

[1]Qin Y, Jin X N, Park H D. Comparison of antioxidant activities in black soybean preparation fermented with various microorganisms [J]. Agricultural Sciences in china, 2010,9(7):1065-1071.

[2]宋以星,杨蕊,杨运华,等.芽孢杆菌B1对黄瓜枯萎病菌的拮抗作用[J].河南科技学院学报,2011,39(3):38-41.
[3]滕安娜.生防木霉拮抗黄瓜枯萎病菌的初步研究[J].农业科技, 2010(1):159-163.
[4]郭敏,刘春燕,陈靠山.拟康氏木霉对蔬菜病原真菌的拮抗作用及对番茄灰霉病的防效的初步研究[J].安徽农学通报, 2008,14(21):156-15.
[5]王光华,周克琴,张秋英,等.拮抗细菌BRF-1对几种植物病原真菌的抗生效果[J].中国生物防治,2003,19(2):73-77.?
[6]段春梅,薛泉宏,赵娟,等.放线菌剂对黄瓜幼苗生长及叶片PPO活性的影响[J].西北农业学报,2010,19(9):48-54.
[7]陈雪丽,王光华,金剑,等.两株芽孢杆菌对黄瓜和番茄根际土壤微生物群落结构影响[J].生态学杂志,2008,27(11): 1895-1900.
[8]王茹华,周宝利,张启发,等.嫁接对茄子根际微生物种群数量的影响[J].园艺学报,2005,32(1):124-126.
[9]Glenn J K, Gold M H. Purification and characterization of an extracellular Mn(Ⅱ)-dependent peroxidase from the lignin-degradingbasidiomycetes, Phancerochaetechrysosporium[J].Arch Biochem Biophys,1985,242:329-341.
[10]Maguson M, Craford D L. Comparison of extracellular peroxidase and esterase-deficient mutants of Streptomyces viridosporus T7A[J].Applied Environmental Microbiology,1992,58:1070-1072.
[11]Dari K, Bechet M, Blondeau R. Isolation of soil streptomyces strains capable of degrading humic acids and analysis of their peroxidase activity[J]. FEMS Microbiol. Ecol,1995,16:115-122.
[12]Simoes D C M, McNeil D, Kristiansen B, et al. Purification and partial characterization of a 1.57 kDa therm ostable esterase from Bacillus stearothermophilus [J].FEMS Microbiol Lett,1997,147:151-156.[13]Naseby D C, Pascual J A, Lynch J M. Effect of biocontrol strains of Trichoderma on plant growth, Pythium ultimum populations, soil microbial communities and soil enzyme activities[J].Appl.Microbiol,2000,88:161-169.
[14]Ye S F, Bowman D, Shi W. Soil microbial community structure and diversity in a turfgrass chronosequence : Land-use change versus turfgrass management[J]. Applied Soil Ecology,2006,34:209-218.
[15]马云华,王秀峰,魏岷,等.黄瓜连作土壤酚酸类物质积累对土壤微生物和酶活性的影响[J].应用生态学报,2005,16(11): 2149-2153.
[16]Woese C R. Bacterial evolution[J]. Microbiol.Rev.,1987,51:221-271.

相似文献/References:

[1]范瑛阁,赵伟,何毅.黄瓜白粉病生防菌的筛选及广谱性测定[J].北方园艺,2013,37(12):116.
　FAN Ying-ge,ZHAO Wei,HE Yi.Screening of Bio-control Agents and Broad Spectrum Measurement on Cucumber Powdery Mildew[J].Northern Horticulture,2013,37(01):116.
[2]秦刚,吴庆丽.辣椒炭疽病生防菌筛选及抗菌活性初步研究[J].北方园艺,2012,36(06):124.
　QIN Gang,WU Qing-li.The Intial Screen of Biological Control and Study of Antimicrobial Activity of Capsicum Anthracnose[J].Northern Horticulture,2012,36(01):124.
[3]范瑛阁,朱丽霞,龚明福.生防菌H1和H2对黄瓜霜霉病的防效测定[J].北方园艺,2012,36(07):149.
　FAN Ying-ge,ZHU Li-xia,GONG Ming-fu.The Biological Control Efficacy of the Strains H1 and H2 Against Cucumber Downy Mildew[J].Northern Horticulture,2012,36(01):149.
[4]周 莹,袁孟娟,韩 军,等.中药材丹参根腐病生防菌的分离与鉴定[J].北方园艺,2015,39(01):145.[doi:10.11937/bfyy.201501039]
　ZHOU Ying,YUAN Meng-juan,HAN Jun,et al.Isolation and Identification of Traditional Chinese Medicine Salvia miltiorrhiza Bunge Root Rot Biocontrol Agents[J].Northern Horticulture,2015,39(01):145.[doi:10.11937/bfyy.201501039]
[5]张俊英,许永利.两株生防菌对连作土壤中黄瓜生长及微生物的影响[J].北方园艺,2015,39(15):28.[doi:10.11937/bfyy.201515006]
　ZHANG Junying,XU Yongli.Effect of Bio-control Bacteria and Fungus on Cucumber Growth and Soil Microbial Population in Continuous Cropping Soil[J].Northern Horticulture,2015,39(01):28.[doi:10.11937/bfyy.201515006]
[6]张雪艳,张亚萍,许帆,等.单一或组合生防菌对田间番茄植株生长和线虫的影响[J].北方园艺,2016,40(07):108.[doi:10.11937/bfyy.201607027]
　ZHANG Xueyan,ZHANG Yaping,XU Fan,et al.Effect of Single or Combination Biocontrol Agent on Growth and Nematode in Tomato Plants[J].Northern Horticulture,2016,40(01):108.[doi:10.11937/bfyy.201607027]
[7]刘 会 清,张 爱 香,马 海 莲,等.生防菌剂与生物有机肥复配对黄瓜抗病促生效果的研究[J].北方园艺,2011,35(05):0.[doi:10.11937/bfyy.201105001]
　LIU Hui-qing,ZHANG Ai -xiang,MA Hai -lian,et al.Study on the G rowth Promoting and Disease Prevention Effect of Biological Control Fungus Applying withOrganic Fertilizer to Cucumber in G reenhouse[J].Northern Horticulture,2011,35(01):0.[doi:10.11937/bfyy.201105001]
[8]陈秀香,王学涛,马金秀,等.生防菌FC21对番茄灰霉病的防效及其促生作用[J].北方园艺,2022,(12):29.[doi:10.11937/bfyy.20215172]
　CHEN Xiuxiang,WANG Xuetao,MA Jinxiu,et al.Control Effect of Biocontrol Strains FC21 Against Tomato Grey Mould and Its Growth-promoting[J].Northern Horticulture,2022,(01):29.[doi:10.11937/bfyy.20215172]

备注/Memo

第一作者简介：尹淑丽（1979-），女，硕士，助理研究员，现主要从事农作物生物防治等研究工作。E-mail: yslbaihelr@126.com。

基金项目：河北省自然科学基金资助项目（C110412）；国家农业成果转化基金资助项目（2010GB2A200041）。
收稿日期：2011-11-03

更新日期/Last Update: 2014-08-22