WANG Man,JIN Can,XU Xue,et al.Cloning and Plant Expression Vector Construction of GA20-oxidase Gene from Pear Dwarfing Mutants and Pingguo Pear[J].Northern Horticulture,2014,38(02):89-94.
梨极矮化突变体与苹果梨GA20ox基因的克隆及植物表达载体的构建
- Title:
- Cloning and Plant Expression Vector Construction of GA20-oxidase Gene from Pear Dwarfing Mutants and Pingguo Pear
- 文章编号:
- 1001-0009(2014)02-0089-06
- 关键词:
- 梨极矮化突变体; GA20-氧化酶基因; 克隆; 序列分析; 植物表达载体
- 分类号:
- S 661. 2
- 文献标志码:
- A
- 摘要:
- 以梨极矮化突变体和苹果梨为试材，应用RT-PCR技术对GA20ox基因进行克隆及序列分析。结果表明：克隆得到梨极矮化突变体和苹果梨GA20-氧化酶基因，依次命名为PuGA20ox和PbGA20ox，其全长均为1 179 bp，分别包含1个完整编码392个氨基酸的开放阅读框（ORF）;对其进行序列分析，并成功构建了植物表达载体，为进一步研究梨矮化突变体和GA20-氧化酶基因功能奠定了基础。
- Abstract:
- Taking pear dwarf mutamt and ‘Pingguo’ pear as test material,by RT-PCR technique,cloning and sequencing of GA20ox gene was studied.The results showed that two GA20-oxidase genes which full-length cDNA consist 1 179 nucleotides and contain complete open reading frame (ORF) which encoding a protein of 392 amino acids residue were isolated from pear dwarfing mutant and ‘Pingguo’ pear.Moreover named that PuGA20ox and PbGA20ox.Cloning and plant expression vector construction of GA20-oxidase gene from pear dwarfing mutants and ‘Pingguo’ pear basis for studying the genetic transformation and function of GA20-oxidase gene.
参考文献/References:
[1]Carrera E,Bou J,Garcia-Martinez J L，et al．Changes in GA 20-oxidase gene expression strongly affect stem length,tuber induction and tuber yield of potato plants[J].Plant,2000(22)：247-256. [2]张超，孙君灵，贾银华,等.外源激素对一个新的棉花极端矮化突变体AS98植株生长和酶活性的影响[J].中国农业科学,2010(7)：1370-1378. [3]Lange T.Molecular biology of gibberellin synthesis[J].Planta,1998,204:409-419. [4]Huang S,Raman A S,Ream J E,et al.Overexpression of 20-oxidase confers a gibberellin-overproduction phenotype in Arabidopsis[J].Plant Physiol，1998，118：773-781. [5]贾敬贤.梨树矮化密植栽培[M].北京：金盾出版社,2005. [6]朴永虎.延边小香水梨（Pyrus ussuriensis Maxim）偶然实生矮化突变体的评价研究[D].延吉：延边大学，2010. [7]金灿.生长调节物质对梨极矮化突变体生长的影响及GA20-氧化酶基因克隆的方法[D].延吉:延边大学,2012. [8]Lange T，Hedden P，Graebe J E.Expression cloning of a gibberellin 20-oxidase，a multifunctional enzyme involved in gibberellin biosynthesis[J].Proc Natl Acad Sci USA,1994,91:8552-8556. [9]Wolbang C M，Chandler P M，Smith J J，et al.Auxin from the developing inflo rescence is required forthe biosynthesis of active gib-berellins in barley stems[J].Plant Physiol,2004,134，769-776. [10]Hedden P,William M.Proebsting.Genetic Analysis of Gibberellin Biosynthesis[J].Plant Physiol,1999,119:365-370. [11]Stephen D.Jackson P E,James,E C.Regulation of Transcript Levels of a Potato Gibberellin20-Oxidase Gene by Light and Phytochrome B[J].Plant Physiol，2000，124:423-430. [12]Gawronska H,Yang Y Y,Furukawa K,et al.Effects of Low Irradiance Stress on Gibberellin Levels in Pea Seedlings[J].Plant Cell Physiol,1995，85:87-90. [13]Peng J，Carol P，Richards D，et al.Arabidopsis GAI gene defines a signaling path-way that negatively regulates gibberellin responses[J].Genes Dev，1997(11)：3194-3205. [14]李伟，陈晓阳，丁霞.高等植物GA20-氧化酶研究进展[J].生命科学，2003,15(1)：42-46.
备注/Memo
第一作者简介：王满（1987-），女，硕士研究生，现主要从事果树遗传育种等研究工作。E-mail：wm_2011@sina.com.