NIE Xiaoying,LU Yacheng,LI Yang,et al.Effects of Bacterial Wilt on the Microbial Community Structure in Rhizosphere Soil and Root Endosphere of Greenhouse-grown Pepper[J].Northern Horticulture,2026,(15):95-104.[doi:10.11937/bfyy.20260471]
青枯病对设施辣椒根际土壤及根内微生物群落结构的影响
- Title:
- Effects of Bacterial Wilt on the Microbial Community Structure in Rhizosphere Soil and Root Endosphere of Greenhouse-grown Pepper
- 文章编号:
- 1001-0009(2026)15-0095-10
- 关键词:
- 设施辣椒; 青枯病; 根际微生物; 根内微生物; Illumina MiSeq
- Keywords:
- greenhouse pepper; bacterial wilt; rhizosphere microbiome; root endosphere microbiome; Illumina miSeq
- 分类号:
- S641.3
- 文献标志码:
- A
- 摘要:
- 以设施大棚内健康与患青枯病辣椒植株为试材，采用Illumina MiSeq高通量测序方法，研究了青枯病对设施辣椒根际土壤及根内微生物群落结构的影响，以期为通过调控根际/根内微生物群落来防控青枯病提供参考依据。结果表明：与健康植株相比，患病植株根际和根内细菌群落的Alpha多样性（OTUs、Chao1、Shannon指数）均显著降低（P<0.05）。患病植株根际和根内变形菌门（Proteobacteria）及青枯雷尔氏菌属（Ralstonia）的相对丰度均显著升高，根内青枯雷尔氏菌属丰度高达99.12%；同时，根际土壤中溶杆菌属（Lysobacter）、土壤杆菌属（Agrobacterium）及根内链霉菌属（Streptomyces）等有益菌属的丰度显著降低。真菌方面，患病植株根际和根内群落均匀度（Shannon指数）显著下降。患病植株根际青霉菌属（Penicillium）丰度显著升高，而根内镰刀菌属（Fusarium）和亡革菌属（Thanatephorus）丰度极显著升高（P<0.01）。PCoA分析表明，健康与患病植株的根际和根内细菌群落、根际真菌群落结构均存在显著差异（P<0.05）。辣椒青枯病的发生伴随着根际和根内微生物群落结构的剧烈变化，表现为细菌多样性显著下降，病原菌青枯雷尔氏菌及潜在致病真菌（如镰刀菌属）大量富集，而多种有益微生物丰度显著降低。
- Abstract:
- Taking healthy and bacterial wilt-infected pepper plants in a facility greenhouse as test materials,Illumina MiSeq high-throughput sequencing was employed to investigate the effects of bacterial wilt on the microbial community structures in the rhizosphere soil and root endosphere of greenhouse pepper,in order to provide references for the prevention and control of bacterial wilt through the regulation of rhizosphere/root endosphere microbial communities.The results showed that compared with healthy plants,the alpha diversity (OTUs,Chao1,and Shannon indices) of bacterial communities in both the rhizosphere and root endosphere of diseased plants was significantly reduced (P<0.05).In diseased plants,the relative abundances of Proteobacteria and Ralstonia (the pathogen causing bacterial wilt) were significantly increased in both the rhizosphere and root endosphere,with the abundance of Ralstonia reaching as high as 99.12% in the root endosphere.Meanwhile,the abundances of beneficial bacterial genera such as Lysobacter and Agrobacterium in the rhizosphere soil,and Streptomyces in the root endosphere,were significantly decreased.Regarding fungi,the community evenness (Shannon index) in both the rhizosphere and root endosphere of diseased plants was significantly reduced.The abundance of Penicillium in the rhizosphere of diseased plants was significantly increased,while the abundances of Fusarium and Thanatephorus in the root endosphere were extremely significantly increased (P<0.01).Principal coordinate analysis (PCoA) revealed significant differences in the bacterial communities of the rhizosphere and root endosphere,as well as in the fungal communities of the rhizosphere,between healthy and diseased plants (P<0.05).The occurrence of pepper bacterial wilt was accompanied by drastic changes in the microbial community structures of both the rhizosphere and root endosphere,characterized by a significant decrease in bacterial diversity,substantial enrichment of the pathogen Ralstonia and potentially pathogenic fungi (e.g.,Fusarium),and a marked reduction in the abundances of various beneficial microorganisms.
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备注/Memo
第一作者简介：聂小英（1997-），女，硕士，农艺师，现主要从事蔬菜育种与栽培技术等研究工作。E-mail:2531142513@qq.com.责任作者：卢发仕(1977-)，男，本科，正高级农艺师，现主要从事蔬菜育种与栽培技术等研究工作。E-mail:674043710@qq.com.〖HTH〗基金项目：广西科技计划项目-平桂区设施辣椒产业科技强链资助项目（桂科农AA2512260009)；国家现代农业产业技术体系广西创新团队桂东蔬菜综合试验站建设资助项目（nycytxgxcxtd-2025-10-04）。收稿日期：2026-02-04