LENG Yunxing,LI Kaijing,LI Xue,et al.Fungicides Identification and Control Agent Screening of a Novel Pathogen Causing Strawberry Anthracnose[J].Northern Horticulture,2026,(15):105-114.[doi:10.11937/bfyy.20260437]
草莓炭疽病新病原菌的鉴定及其防控药剂筛选
- Title:
- Fungicides Identification and Control Agent Screening of a Novel Pathogen Causing Strawberry Anthracnose
- 文章编号:
- 1001-0009(2026)15-0105-10
- 分类号:
- S668.4
- 文献标志码:
- A
- 摘要:
- 以贵阳地区采集的草莓炭疽病典型病样为试材，采用组织分离法、病原形态学观察、多基因序列系统发育分析及柯赫氏法则验证，研究了草莓炭疽病的致病菌种类及其生物学特性；并采用菌丝生长速率法，研究了9种植物源活性物质及其复配组合对该病原菌的室内抑制活性，以期为草莓炭疽病的绿色防控提供参考依据。结果表明：引起贵阳地区草莓炭疽病的病原菌为喀斯特炭疽菌（Colletotrichum karstii）；室内毒力测定中，厚朴酚与蛇床子素抑菌活性最强，EC50值分别为9.581 9 mg·L-1和13.136 2 mg·L-1；二者以质量比9∶1复配后增效作用显著，共毒系数达192.54。综上，该研究明确Colletotrichum karstii为中国草莓炭疽病新病原，并筛选出厚朴酚与蛇床子素按9∶1复配这一增效显著的组合，为该病害的绿色防控提供了病原学依据与药剂应用方案。
- Abstract:
- Taking typical strawberry anthracnose samples collected from Guiyang area as test materials,the pathogenic species and its biological characteristics were investigated through tissue isolation,morphological observation,multi-gene phylogenetic analysis and Koch′s postulates verification.The mycelial growth rate method was employed to evaluate the in vitro inhibitory activities of nine botanical active compounds and their combinations against the pathogen,in order to provide references for the green prevention and control of strawberry anthracnose.The results showed that the pathogen causing strawberry anthracnose in Guiyang was identified as Colletotrichum karstii.The in vitro toxicity tests revealed that magnolol and osthole exhibited the strongest antifungal activities,with EC50 values of 9.581 9 mg·L-1 and 13.136 2 mg·L-1,respectively.A mixture of magnolol and osthole at a mass ratio of 9∶1 demonstrated significant synergistic effect,with a co-toxicity coefficient of 192.54.In conclusion Colletotrichum karstii was confirmed as a novel pathogen of strawberry anthracnose in China and a highly synergistic mixture of honokiol and osthole (9∶1) was identified.These findings provide etiological evidence and practical fungicide strategies for the green control of this disease.
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相似文献/References:
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备注/Memo
第一作者简介：冷云星(1975- )，女，本科，高级农艺师，现主要从事果树技术推广等研究工作。E-mail:748622617@qq.com.责任作者：尹显慧（1978-），女，博士，教授，现主要从事有害生物绿色防控及农产品质量安全等研究工作。E-mail:xhy@gzu.edu.cn.基金项目：贵州特色水果产业技术体系资助项目；贵州省科技创新人才团队资助项目（黔科合平台人才-CXTD［2023］015）。收稿日期：2026-02-02