GAO Pan.Regulation of Bulblet by Sucrose and Light-dark Conditions During in vitro Culture of ‘Yandan No.1’[J].Northern Horticulture,2026,(15):18-27.[doi:10.11937/bfyy.20260077]
‘延丹1号’组培快繁中蔗糖与光暗对鳞茎的调控
- Title:
- Regulation of Bulblet by Sucrose and Light-dark Conditions During in vitro Culture of ‘Yandan No.1’
- 文章编号:
- 1001-0009(2026)15-0018-10
- Keywords:
- Lilium pumilum DC.; tissue culture; sucrose concentration; bulblet enlargement; light and dark culture
- 分类号:
- S682.2+9
- 文献标志码:
- A
- 摘要:
- 以赏食两用山丹品种‘延丹1号’中层鳞片为试材，采用三因素试验设计（7个蔗糖浓度梯度30~90 g·L-1、光暗2个处理、4个测量时间点），结合三因素方差分析与简单效应分析，研究了蔗糖浓度与光暗条件对‘延丹1号’组培快繁中鳞茎增重、膨大、分化、生根及生叶等指标的影响，以期为‘延丹1号’规模化生产提供参考依据。结果表明：蔗糖浓度、光暗处理与培养时间对各生长指标均存在显著或极显著影响，并在多数指标上表现出交互作用；光照条件总体有利于不定芽分化、根和叶形成，黑暗条件在部分中高蔗糖处理下可促进鲜质量或直径增加，但促进效应受蔗糖浓度和培养时间限制。光照下40 g·L-1蔗糖处理（A2B1）分化表现较稳定，60 g·L-1蔗糖处理（A4B1）生根表现较好，50 g·L-1蔗糖处理（A3B1）在鲜质量、膨大和稳定性方面综合表现较优；若以60 d成叶效果为依据，A2B1较优。综合增重、膨大、分化、生根、生叶及变异系数，50 g·L-1蔗糖+光照培养可作为‘延丹1号’优质鳞茎生产的推荐组合。
- Abstract:
- Taking middle-layer scales of the dual-purpose ornamental and edible cultivar ‘Yandan No.1’ (Lilium pumilum DC.) as test material,a three-factor experimental design-seven sucrose concentrations (30-90 g·L-1),light and dark two conditions.Four sampling time points was employed,and three-way ANOVA together with simple-effects analysis was used to investigate the effects of sucrose concentration and light-dark conditions on bulblet weight gain,enlargement,differentiation,rooting and leaf emergence during tissue-culture rapid propagation,in order to provide a reference for the large-scale production of ‘Yandan No.1’.The results showed that sucrose concentration,light/dark treatment,and culture time had significant or highly significant effects on all growth indicators,and interactions were observed for most indicators.Light conditions generally favored adventitious bud differentiation,root formation,and leaf emergence,whereas dark conditions promoted fresh weight or diameter increase in some medium-and high-sucrose treatments; however,these promotive effects were influenced by sucrose concentration and culture time.Under light conditions,40 g·L-1 sucrose treatment (A2B1) showed relatively stable differentiation,60 g·L-1 sucrose treatment (A4B4) showed better rooting performance,and 50 g·L-1 sucrose treatment (A3B1) showed better comprehensive performance in fresh weight,enlargement,and stability.When leaf emergence at 60 days was used as the criterion,A2B1 performed better.Considering weight gain,enlargement,differentiation,rooting,leaf emergence,and coefficient of variation,50 g·L-1 sucrose under light culture could be recommended as a suitable combination for high-quality bulblet production of ‘Yandan No.1’.
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备注/Memo
作者简介：高攀（2001-），女，硕士研究生，研究方向为百合资源开发利用。E-mail：3308622906@qq.com.基金项目：陕西省重大科技专项资助项目（2022ZDLNY03-10）。收稿日期：2026-01-08