GAN Yonghui,JIANG Xiaotong.Effects of Selenium Application on the Accumulation of Lead and Mercury in Anoectochilus roxburghii[J].Northern Horticulture,2026,(13):63-69.[doi:10.11937/bfyy.20254471]
施硒对金线莲铅、汞积累的影响
- Title:
- Effects of Selenium Application on the Accumulation of Lead and Mercury in Anoectochilus roxburghii
- 文章编号:
- 1001-0009(2026)13-0063-07
- Keywords:
- Anoectochilus roxburghii; sodium selenite; lead; mercury; stress resistance
- 分类号:
- S567.5
- 文献标志码:
- A
- 摘要:
- 以‘红霞’金线莲为试材,采用不同剂量亚硒酸钠进行种苗繁殖培养后林下移栽种植,研究在林下种植植株体内铅和汞的积累情况下,硒对植株铅、汞吸收的影响,以期为安全铅、汞含量金线莲植株栽培生产提供参考依据。结果表明:培养基施用5.5~8.0 mg·L-1硒元素进行培养繁殖苗后林下移栽种植,显著提高植株成活率和产量,最高分别提高15.2%和14.9%;该浓度硒施用显著抑制金线莲植株对铅和汞的吸收与转运,最高分别降低吸收率10.2%和23.5%、向地上部转运效率19.8%和21.2%;施用5.5 mg·L-1浓度硒极显著降低植株细胞膜脂过氧化产物丙二醛含量18.2%、极显著提高脯氨酸含量35.5%。综上,金线莲培养基施硒繁殖可提高种苗在林下移栽环境的抗逆性、增加其产量,且减少植株对铅和汞的吸收和转运。
- Abstract:
- Taking ‘Hongxia’ Anoectochilus roxburghii as the test material,the seedlings were cultivated by adding different doses of sodium selenite for propagation,and then transplanted and planted in the forest area.The effects of selenium on the absorption of lead and mercury by the plants when lead and mercury were accumulated in the plants′ bodies were investigated,in order to provide a reference for the cultivation and production of safe lead and mercury content on Anoectochilus roxburghii.The results showed that applying 5.5-8.0 mg·L-1 selenium to the culture medium for propagating seedlings and planting them under the forest significantly increased plant survival rate and yield,with the highest increases of 15.2% and 14.9%.Significantly inhibited the absorption and transport of lead and mercury by the plants,reducing absorption rates by 10.2% and 23.5%,and decreasing their above ground transport efficiency by 19.8% and 21.2%.The application of 5.5 mg·L-1 selenium significantly reduced the content of malondialdehyde (MDA) by 18.2% and significantly increased the content of proline by 35.5%.The comprehensive results indicated that selenium application in the culture medium of Anoectochilus roxburghii could improve the stress resistance of seedlings in the understory transplanting environment,increased their yield,and reduced the absorption and transport of lead and mercury by plants.
参考文献/References:
[1]张慧玲,党菲,李紫竹,等.土壤-植物系统“硒-汞拮抗”研究进展综述[J].南京大学学报(自然科学版),2017,53(5):903-911.[2]周迪,王梅,高学振,等.铅污染农田土壤修复后的环境容量及风险演变[J].农业环境科学学报,2024,43(11):2583-2594.[3]许秀艳,朱红霞,于建钊,等.环境中汞化学形态分析研究进展[J].环境化学,2015,34(6):1086-1094.[4]侯超文,石雨荷,李晓玲,等.重金属胁迫下六种百合科植物响应特性及富集特性的研究进展[J].北方园艺,2025(12):134-140.[5]安立进,赵礼鹏,魏奥,等.硒阻控作物吸收重金属机制研究进展[J].华中农业大学学报,2024,43(4):112-120.[6]DESOKY E M,MERWAD A M A,ABO EL-MAATI M F,et al.Physiological and biochemical mechanisms of exogenously applied selenium for alleviating destructive impacts induced by salinity stress in bread wheat[J].Agronomy,2021,11(5):926.[7]KHALOFAH A,MIGDADI H,EL-HARTY E.Antioxidant enzymatic activities and growth response of quinoa (Chenopodium quinoa Willd) to exogenous selenium application[J].Plants,2021,10(4):719.[8]陈利云,王弋博,李三相,等.硒元素对铅、汞胁迫下紫花苜蓿生长的影响[J].中国草地学报,2016,38(5):109-114.[9]SOBRINO-PLATA J,CARRASCO-GIL S,ABADA J,et al.The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis[J].Metallomics,2014,6(2):356-366.[10]ROTRUCK J T,POPE A L,GANTHER H E,et al.Selenium:Biochemical role as a component of glutathione peroxidase[J].Science,1973,179(4073):588-590.[11]周鑫斌,施卫明,杨林章.叶面喷硒对水稻籽粒硒富集及分布的影响[J].土壤学报,2007,44(1):73-78.[12]MENG B,FENG X B,QIU G G,et al.Localization and speciation of mercury in brown rice with implications for pan-Asian public health[J].Environmental Science & Technology,2014,48(14):7974-7981.[13]孔繁越.中药材重金属限量标准和农残限量标准研究及标准制定相关建议[D].北京:北京中医药大学,2017.[14]程伟青,吴水华,邓笑治.不同栽培方式对金线莲中微量元素的影响[J].安徽农业科学,2017,45(20):123-125.[15]林晨.不同栽培模式金线莲药材中微量元素、植物激素和甾醇的分析[D].福州:福建医科大学,2015.[16]甘勇辉,王水琦.金线莲施硒的吸收与积累规律[J].中国土壤与肥料,2023(8):214-221.[17]曾碧玉,苏明华,陈清西,等.金线莲多糖的提取工艺优化[J].热带作物学报,2016,37(3):615-621.[18]王澄林,张勋,陈抒云,等.金线莲总黄酮的最佳超声提取工艺[J].福建中医药,2017,48(1):40-42.[19]王娟.干法灰化和微波消解石墨炉原子吸收法对茶叶中铅含量测定的比较[J].湖南农业科学,2014(9):55-57.[20]王振旭,程海芳,赵芳,等.食品中汞含量测定方法的优化研究[J].食品研究与开发,2018,39(6):159-162.[21]李小方,张志良.植物生理学实验指导[M].5版.北京:高等教育出版社,2016.[22]毋赟,王文雄.汞在海洋浮游植物中的生物累积和毒性效应[J].生态毒理学报,2014,9(5):810-818.[23]陶惠,陈颖,刘艳伟,等.贵州万山汞矿区植物中汞的累积特征[J].环境化学,2022,41(12):4047-4056.[24]郭静成,尹顺平.硒对高等植物中谷胱甘肽过氧化物酶活性及谷胱甘肽含量的影响[J].西北植物学报,1998,18(4):533-537.[25]董卫华.Na2SeO3对麦苗谷胱甘肽过氧化物酶影响的研究[D].重庆:西南农业大学,2002.[26]樊俊飞.不同基因型小麦对硒、铅和镉胁迫响应的差异研究[D].武汉:华中农业大学,2018.[27]张海英,韩涛,田磊,等.草莓叶面施硒对其重金属镉和铅积累的影响[J].园艺学报,2011,38(3):409-416.[28]张璐,周鑫斌,苏婷婷.叶面施硒对水稻各生育期镉汞吸收的影响[J].西南大学学报(自然科学版),2017,39(7):50-56.[29]刘笑笑,张振都,张奇,等.桑黄子实体中硒元素及重金属含量分析[J].北方园艺,2022(5):104-111.[30]张翠翠,常介田,赵鹏.叶面施硒对西瓜镉和铅积累的影响[J].华北农学报,2013,28(3):159-163.[31]刘燕,蒋光霞.硒对镉、铅复合污染下油菜重金属抗性的影响[J].安徽农业科学,2010,38(21):11096-11098.[32]巩正,崔雪梅,王彦,等.硒、铅胁迫对油菜生理生化指标的影响[J].湖北工程学院学报,2016,36(3):51-54.[33]蔡阳光,覃剑锋,陈国爱,等.外源硒对岚皋花魔芋生长发育的影响[J].山西农业大学学报(自然科学版),2018,38(6):35-42.
相似文献/References:
[1]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2014,38(11):158.
YANG Hui-fang,LIANG Xin-an,CHANG Jie-tian,et al.Effects of Foliar Spraying of Selenium Fertilizer on Selenium Enrichment of Different Vegetables and Yield[J].Northern Horticulture,2014,38(13):158.
[2]韩晓红,张林成,段春红.金线莲练苗及移栽技术研究[J].北方园艺,2013,37(10):78.
HAN Xiao-hong,ZHANG Lin-cheng,DUAN Chun-hong.Study on Hardening seedling and Transplanting Techniques of Anoectochilus roburghii[J].Northern Horticulture,2013,37(13):78.
[3]翁 梁,温 鲁.硒和钙对蛹虫草活性物质含量的影响[J].北方园艺,2012,36(22):159.
WENG Liang,WEN Lu.Effects of Selenium and Calcium on the Content of Cordyceps militaris Active Substances[J].Northern Horticulture,2012,36(13):159.
[4]王文举,王振平.亚硒酸钠对“红地球”葡萄耐热性及果实品质的影响[J].北方园艺,2012,36(24):47.
WANG Wen-ju,WANG Zhen-ping.The Effect of Sodium Selenite on Heat-resistance and Berry Quality of Red Globe Grape[J].Northern Horticulture,2012,36(13):47.
[5]韩晓红,罗明英,欧阳志成,等.金线莲原球茎增殖研究[J].北方园艺,2012,36(24):144.
HAN Xiao-hong,LUO Ming-ying,OUYANG Zhi-cheng,et al.Study on the Proliferation of Protocorm-like Bodies in Anoectochilus roburghii[J].Northern Horticulture,2012,36(13):144.
[6]唐军荣,谭辛夷,孙正海,等.金线莲ISSR-PCR反应体系建立[J].北方园艺,2014,38(18):118.
TANG Jun-rong,TAN Xin-yi,SUN Zheng-hai,et al.Establishment of ISSR-PCR Reaction System for Anoectochilus roxburghii[J].Northern Horticulture,2014,38(13):118.
[7]魏翠华,谢 宇,秦建彬,等.光照强度对金线莲生长及产量的影响[J].北方园艺,2015,39(12):139.[doi:10.11937/bfyy.201512036]
WEI Cui-hua,XIE Yu,QIN Jian-bin,et al.Effect of Light Intensity on the Growth and Yield of Anoectochilus roxburghii[J].Northern Horticulture,2015,39(13):139.[doi:10.11937/bfyy.201512036]
[8]封 应 丽,余 元 涛,杨 春 桃,等.植物生长物质对金线莲离体再生的影响[J].北方园艺,2010,34(07):0.[doi:10.11937/bfyy.201007053]
FENG Ying -li,YU Yuan -tao,YANG Chun -tao,et al.Effect of Plant Growth Substance on Regeneration of Anoectochilus formosanus[J].Northern Horticulture,2010,34(13):0.[doi:10.11937/bfyy.201007053]
[9]黄 勇.金线莲组织培养新体系建立及优化[J].北方园艺,2010,34(13):0.[doi:10.11937/bfyy.2010013065]
[10]甘金佳,蒋水元,李虹,等.广西金线莲野生资源现状及保护策略[J].北方园艺,2016,40(09):160.[doi:10.11937/bfyy.201609043]
GAN Jinjia,JIANG Shuiyuan,LI Hong,et al.Current Situation of Wild Resources and Protection Strategy of Anoectochilus roxburghii in Guangxi[J].Northern Horticulture,2016,40(13):160.[doi:10.11937/bfyy.201609043]
备注/Memo
第一作者简介:甘勇辉(1967-),男,本科,副教授,现主要从事植物栽培与生理生态等研究工作。E-mail:yhgfjzz@163.com.基金项目:福建省科技计划资助项目(2018N2002);漳州市自然科学基金资助项目(ZZ2019J08)。收稿日期:2025-12-31