CHI Xiangdan,WANG Yongzhuo,ZHAO Yanfei,et al.Growth and Physiological Responses to High Salt Stress in Seedlings of Melon Germplasms[J].Northern Horticulture,2026,(15):56-66.[doi:10.11937/bfyy.20254394]
甜瓜种质幼苗在高盐胁迫下的生长及生理响应
- Title:
- Growth and Physiological Responses to High Salt Stress in Seedlings of Melon Germplasms
- 文章编号:
- 1001-0009(2026)15-0056-11
- 分类号:
- S652
- 文献标志码:
- A
- 摘要:
- 以13份甜瓜种质资源为试材，通过测定生长与生理指标，结合主成分分析和聚类分析，综合评价其苗期耐盐性，以期为耐盐育种提供参考依据。结果表明：盐胁迫显著抑制幼苗生长，株高降幅达15%~57%，丙二醛（MDA）含量上升，脯氨酸（Pro）含量大量积累，叶绿素含量下降。耐盐材料能维持较高的超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性，三者与MDA含量呈极显著负相关。主成分分析提取出3个主成分（累计贡献率86.039%），结合聚类分析将材料分为5个耐盐等级，其中8号（‘2025-42’）与12号（“梦蜜”）为高耐盐材料，4号与11号为高敏感材料。因此甜瓜耐盐性多受生理过程协同调控，其中抗氧化酶系统的协同防御能力为核心机制。
- Abstract:
- Thirteen melon germplasm resources were used as the test materials to comprehensively evaluate their salt tolerance at the seedling stage by measuring growth and physiological indicators，combined with principal component analysis (PCA)and cluster analysis,in order to provide references for salt-tolerant breeding.The results showed that salt stress significantly inhibited seedling growth，with plant height reduction ranging from 15% to 57%.Malondialdehyde(MDA) content increased，proline(Pro) content accumulated substantially，and chlorophyll content decreased.Salt-tolerant materials maintained higher activities of superoxide dismutase (SOD)，peroxidase (POD)，and catalase (CAT)，and the activities of these three enzymes were significantly negatively correlated with MDA content.PCA extracted three principal components with a cumulative contribution rate of 86.039%.Cluster analysis classified the 13 materials into five salt-tolerance grades，among which No.8 (‘2025-42’) and No.12 (‘Mengmi’) were highly salt-tolerant，while No.4 and No.11 were highly sensitive.In conclusion，salt tolerance in melon was synergistically regulated by multiple physiological processes，with the coordinated defense capability of the antioxidant enzyme system serving as the core mechanism.
参考文献/References:
［1］杨劲松,姚荣江,王相平,等.中国盐渍土研究:历程、现状与展望［J］.土壤学报,2022,59(1):10-27.［2］赵作章,陈劲松,彭尔瑞,等.土壤盐渍化及治理研究进展［J］.中国农村水利水电,2023(6):202-208.［3］李建国,濮励杰,朱明,等.土壤盐渍化研究现状及未来研究热点［J］.地理学报,2012,67(9):1233-1245.［4］ALONSO S,GAUTAM K,IGLESIAS-MOYA J,et al.High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepo［J］.Frontiers in Plant Science,2025（16）:1548576.［5］齐琪,马书荣,徐维东.盐胁迫对植物生长的影响及耐盐生理机制研究进展［J］.分子植物育种,2020,18(8):2741-2746.［6］刘杉琦.耐盐性植物促生菌群的构建及其对盐胁迫下大豆生长的调控机制研究［D］.哈尔滨:东北农业大学,2025.［7］金刘彪.农安县盐渍土电阻率时空变异特性研究［D］.长春:吉林建筑大学,2024.［8］王炳春.现阶段我国开展盐碱地综合利用对策研究［J］.中国农垦,2024(1):52-54.［9］卢文静.NaCl胁迫对两个木槿品种幼苗生理生化特性的影响［D］.泰安:山东农业大学,2021.［10］沙凤霞,周梓晗,官振丹,等.盐、碱胁迫对 “宁杞菜1号” 生理特性的影响［J］.北方园艺,2026(3):43-51.［11］郗孟雨,李东霞,李腾云,等.二氢卟吩铁对盐胁迫下黄瓜种子萌发和幼苗的影响［J］.北方园艺,2025(22):35-43.［12］王春梅,杨晓旭,张泰峰,等.外源尿囊素对盐胁迫下菜豆种子生理特性的影响［J］.北方园艺,2025(20):42-48.［13］卿东山,何长征,罗玉松,等.甜瓜种质资源遗传多样性分析与核心种质构建［J］.湖南农业大学学报(自然科学版),2025,51(2):36-43.［14］林德佩.中国栽培甜瓜植物的起源、分类及进化［J］.中国瓜菜,2010,23(4):34-36.［15］YANG W L,LING Y M,LI M H,et al.Screening and identification of saline-tolerant germplasm in melon［J］.Agriculture,2023,13(11):2051.［16］张海波,崔继哲,曹甜甜,等.大豆出苗期和苗期对盐胁迫的响应及耐盐指标评价［J］.生态学报,2011,31(10):2805-2812.［17］姚静,施卫明.盐胁迫对番茄根形态和幼苗生长的影响［J］.土壤,2008,40(2):279-282.［18］钱玥,饶良懿.盐碱胁迫对枸杞幼苗生长与叶绿素荧光特性的影响［J］.森林与环境学报,2022,42(3):271-278.［19］齐月.不同砧木‘灯笼红’甜瓜幼苗耐盐碱胁迫生理特性研究［D］.呼和浩特:内蒙古农业大学,2023.［20］林佳佳.盐胁迫下厚皮甜瓜的生理响应及转录组分析［D］.南宁:广西大学,2024.［21］李雨霏,王建全,武子桐,等.不同甜瓜自交系耐盐性评价［J］.中国蔬菜,2025(9):89-96.［22］侯尹婕,黄欣,冯梦娇,等.十二份西瓜种质资源耐盐性鉴定［J］.北方园艺,2025(7):1-8.［23］赵映雪.NaCl胁迫对沙葱幼苗根和叶片生理生化基础的影响［D］.呼和浩特:内蒙古农业大学,2019.［24］薛洋,赵胜杰,何玉敏,等.瓜菜作物耐盐性研究进展［J］.中国瓜菜,2023,36(12):1-8.［25］李世玉,程登虎,闫星,等.26份甜瓜材料耐盐性鉴定与评价［J］.中国瓜菜,2022,35(9):23-30.［26］李颖,张树航,郭燕,等.211份板栗种质资源花序表型多样性和聚类分析［J］.中国农业科学,2020,53(22):4667-4682.［27］罗玢晔,陈胜艳,杨宇佳,等.盐胁迫下野菊离子的吸收转运情况及相关基因的表达［J］.东北林业大学学报,2024,52(2):68-74,83.［28］GONG X L,CHAO L,ZHOU M,et al.Oxidative damages of maize seedlings caused by exposure to a combination of potassium deficiency and salt stress［J］.Plant and Soil,2011,340(1):443-452.［29］FAROOQ M,GOGOI N,HUSSAIN M,et al.Effects,tolerance mechanisms and management of salt stress in grain legumes［J］.Plant Physiology and Biochemistry,2017,118:199-217.［30］GILL S S,TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants［J］.Plant Physiology and Biochemistry,2010,48(12):909-930.［31］张子健.盐胁迫对甜瓜幼苗光合特性及根系生长与根际微域特性的影响［D］.呼和浩特:内蒙古农业大学,2021.［32］朱虹,祖元刚,王文杰,等.逆境胁迫条件下脯氨酸对植物生长的影响［J］.东北林业大学学报,2009,37(4):86-89.［33］刘婷婷.基于转录组解析外源褪黑素缓解黄瓜盐胁迫的分子机制［D］.太谷:山西农业大学,2022.［34］孙凤岭,陈昆,姜涛,等.盐胁迫对西瓜幼苗光合色素、光合特性及抗氧化保护酶系统的影响［J］.园艺与种苗,2022,42(12):4-6.［35］刘迎,鲁倩君,章智钧,等.不同耐盐葡萄砧木叶片膜脂过氧化及抗氧化系统对NaCl胁迫的响应［J］.分子植物育种,2026,24(1):255-265.［36］王菲菲,张胜忠,杨贵华,等.331份花生种质苗期耐盐性综合评价和强耐盐种质鉴选［J］.作物学报,2026,52(1):279-294.［37］于颖珊,尹萌萌,黄战,等.西瓜苗期耐盐性评价体系的建立及耐盐种质筛选［J］.中国蔬菜,2025(11):52-60.［38］马小红,焦慧,虎志瑞,等.番茄种质种子萌发期耐盐性综合评价及关键指标筛选［J］.云南大学学报(自然科学版),2025,47(6):1176-1184.
相似文献/References:
[1]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[2]石秋梅,邓翻云,吴敏言,等.罗布麻和大叶白麻种子萌发及幼苗生长耐盐性研究[J].北方园艺,2014,38(12):128.
SHI Qiu-mei,DENG Fan-yun,WU Min-yan,et al. Study on Salt Tolerance of Apocynum venetum Linn.and Poacynum hendersonii(Hook.f.) Woodson at Stages of Seed Germination and Seedlings Growth[J].Northern Horticulture,2014,38(15):128.
[3]张 芬,张 波,田丽萍,等.盐胁迫对番茄幼苗叶片光合特性及叶绿素和β-胡萝卜素含量的影响[J].北方园艺,2014,38(11):15.
ZHANG Fen,ZHANG Bo,TIAN Li-ping,et al.Effect of Salt Stress on Photosynthetic Characteristic and the Content of Chlorophyll and β-carotene in Tomato Seedling Leaves[J].Northern Horticulture,2014,38(15):15.
[4]常青山,张利霞,郑轶琦,等.盐胁迫下钙离子对苜蓿种子萌发的影响[J].北方园艺,2014,38(10):64.
CHANG Qing-shan,ZHANG Li-xia,ZHENG Yi-qi,et al.Effects of Ca2+?on Seed Germination Characteristics of Medicago sativa Under Salt Stress[J].Northern Horticulture,2014,38(15):64.
[5]杨 凯,孙迎坤,谭 雯,等.盐胁迫对香石竹种子萌发及幼苗生长的影响[J].北方园艺,2013,37(22):86.
YANG Kai,SUN Ying-kun,TAN Wen,et al.Effects of Salt Stress on Seeds Germination and Seedling Growth of Carnation[J].Northern Horticulture,2013,37(15):86.
[6]张 娇,张大治,马 艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014,38(14):65.
ZHANG Jiao,ZHANG Da-zhi,MA Yan,et al.Effect of NaCl Simulation of Salt Stress on Seed Germination and Seedling Growth of Ammopiptanthus mongolicus[J].Northern Horticulture,2014,38(15):65.
[7]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(15):1.
[8]何会流.外源水杨酸对盐胁迫下凤仙花种子萌发特性的影响[J].北方园艺,2013,37(13):75.
HE Hui-liu.Effect of Exogenous Salicylic Acid on the Seeds Germination Characters of Impatiens balsamina L.Under NaCl Stress[J].Northern Horticulture,2013,37(15):75.
[9]刘 杰.盐碱胁迫对向日葵体内矿质营养的影响[J].北方园艺,2014,38(02):1.
LIU Jie.Effects of Simulated Salt and Alkali Conditions on the Mineral Nutrition of Helianthus annuusL.[J].Northern Horticulture,2014,38(15):1.
[10]张芬,张波,田丽萍,等.盐胁迫对加工番茄叶片番茄红素[J].北方园艺,2014,38(04):82.
ZHANG Fen,ZHANG Bo,TIAN Li-ping,et al.Effect of Salt Stress on Expression of Lycopene Cyclase Gene in Processing Tomato Leaves[J].Northern Horticulture,2014,38(15):82.
备注/Memo
第一作者简介：迟翔丹（1996-），女，硕士，研究实习员，现主要从事西甜瓜遗传育种及栽培技术等研究工作。E-mail：939375151@qq.com.责任作者：陈莹（1978-），女，硕士，研究员，现主要从事西甜瓜遗传育种及栽培技术等研究工作。E-mail：56429596@qq.com.基金项目：吉林省科学技术技厅项目-吉林省重点实验室（吉林省园艺植物遗传育种与生物技术重点实验室）建设资助项目（YDZJ202502CXJD096)；吉林省财政厅资助项目(SC20260016)。收稿日期：2025-12-23