WANG Shanshan,ZHAO Chenhui,WANG Lixin,et al.Identification and Expression Analysis of Di19 Gene Family in Apple Under Drought Stress[J].Northern Horticulture,2026,(13):28-37.[doi:10.11937/bfyy.20254310]
苹果Di19基因家族鉴定及其在干旱胁迫下的表达分析
- Title:
- Identification and Expression Analysis of Di19 Gene Family in Apple Under Drought Stress
- 文章编号:
- 1001-0009(2026)13-0028-10
- 分类号:
- S661.1
- 文献标志码:
- A
- 摘要:
- 以‘金红’苹果（Malus domestica L.）为试材，采用多种生物信息学分析方法，研究了苹果Di19基因家族成员的蛋白理化性质、染色体定位、基因结构、保守基序、系统发育、共线性和顺式作用元件、基因表达组织特性及响应干旱胁迫功能，以期为进一步解析苹果Di19基因在响应干旱胁迫过程的功能和作用机制提供参考依据。结果表明：在苹果基因组中共鉴定出7个苹果Di19基因家族成员，分布在7条染色体上，均具有zf-Di19和Di19-C保守结构域；共线性分析表明苹果Di19家族成员之间存在5对片段重复基因，在进化过程中受纯化选择；系统发育分析将苹果、拟南芥、梨、桃和番茄的Di19基因分为3个亚族；苹果Di19基因启动子中含有响应植物非生物胁迫、生长发育、激素应答顺式作用元件。转录组分析和RT-qPCR分析结果表明，苹果Di19基因存在组织特异性表达，且在干旱胁迫处理下MdDi19-3、MdDi19-4和MdDi19-7差异表达，表明这3个基因在干旱胁迫过程中发挥重要作用。
- Abstract:
- Taking ‘Jinhong’ apple (Malus domestica L.) as the test material,this study employed various bioinformatics analysis methods to investigate the physicochemical properties,chromosomal localization,gene structure,conserved motifs,phylogenetic relationships,collinearity,cis-regulatory elements,tissue-specific expression patterns,and drought stress response functions of Di19 gene family members，in order to provide references for further elucidating the functional roles and mechanisms of apple Di19 genes in drought stress response processes.The results showed that seven members of Di19 gene family in apple were identified across seven chromosomes,all possessing the zf-Di19 and Di19-C conserved domains.Collinearity analysis revealed five pairs of segmental repeat genes among Di19 family members in apple,which underwent purifying selection during evolution.Phylogenetic analysis classified the Di19 genes from Malus domestica,Arabidopsis thaliana,Pyrus communis,Prunus persica and Solanum lycopersicum into three subfamilies.The promoter of Di19 genes in apple contains cis-acting elements that respond to abiotic stress,growth and development,and hormone response.Transcriptomic and RT-qPCR analyses demonstrated tissue-specific expression of apple Di19 genes,with MdDi19-3,MdDi19-4,and MdDi19-7 showing differential expression under drought stress,indicating their crucial roles in drought response processes.
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备注/Memo
第一作者简介：王珊珊（1983-），女，硕士，助理研究员，现主要从事果树种质资源与抗逆分子育种等研究工作。E-mail:826596552@qq.com.责任作者：梁英海（1976-），男，博士，研究员，现主要从事果树种质资源与抗逆分子育种等研究工作。E-mail:yinghailiang365@163.com.基金项目：农业农村部种质资源保护资助项目(22250291)；科技部、财政部国家科技资源共享服务平台资助项目(NHGRC2025-NH09)。收稿日期：2025-12-16