FU Jiaojiao,YANG Jiale,GAO Shansong,et al.Sensitivity of Zantedeschia elliottiana ‘Jingcai Yangguang’ to Different Antibiotics[J].Northern Horticulture,2026,(13):54-62.[doi:10.11937/bfyy.20254246]
黄花马蹄莲‘京彩阳光’对不同抗生素的敏感性分析
- Title:
- Sensitivity of Zantedeschia elliottiana ‘Jingcai Yangguang’ to Different Antibiotics
- 文章编号:
- 1001-0009(2026)13-0054-09
- 关键词:
- 黄花马蹄莲‘京彩阳光’; 形成率; 抗生素敏感性; 遗传转化
- Keywords:
- Zantedeschia elliottiana ‘Jingcai Yangguang’; formation rates; sensitivity to antibiotics; genetic transformation
- 分类号:
- S682
- 文献标志码:
- A
- 摘要:
- 以黄花马蹄莲‘京彩阳光’（Zantedeschia elliottiana ‘Jingcai Yangguang’）的无菌块茎切片为试材，采用植物组织培养和抗生素敏感性分析方法，研究了潮霉素（Hyg）、卡那霉素（Kan）、头孢霉素（Cef）和特美汀（Tim）对其愈伤组织诱导、丛生芽和根形成的影响，以期筛选适用于黄花马蹄莲遗传转化体系的选择和抑菌抗生素，为黄花马蹄莲遗传转化体系中抗生素种类和浓度的确定提供参考依据。结果表明：‘京彩阳光’对4种抗生素的敏感性排序为Hyg>Kan>Cef>Tim；不同组织器官的敏感性表现为愈伤组织＞根＞丛生芽。从块茎切片愈伤组织、丛生芽和根的形成情况来看，卡那霉素的作用浓度是潮霉素的10倍，头孢霉素和特美汀是潮霉素的100倍。40 mg·L-1卡那霉素对愈伤组织的抑制率为93.76%，形成率为5.65%；丛生芽的抑制率为49.55%，形成率为29.56%；10 mg·L-1潮霉素对愈伤组织的抑制率为97.83%，形成率为1.96%；对丛生芽的抑制率为55.38%，形成率为26.15%。10 mg·L-1潮霉素和40 mg·L-1卡那霉素可作为‘京彩阳光’以块茎切片为外植体的遗传转化体系的筛选剂。300 mg·L-1头孢霉素作用下，愈伤组织的抑制率为80.76%；形成率为17.43%；丛生芽的抑制率为13.91%，形成率为50.45%；300 mg·L-1特美汀作用下，愈伤组织的抑制率为73.18%，形成率为24.29%；丛生芽的抑制率为16.29%，形成率为49.05%。300 mg·L-1的特美汀或头孢霉素可作为遗传转化体系中农杆菌生长繁殖的抑制剂。
- Abstract:
- Taking sterile tuber slices of yellow calla lily ‘Jingcai Yangguang’ (Zantedeschia elliottiana ‘Jingcai Yangguang’) as the test material,this study employed plant tissue culture and antibiotic sensitivity analysis to investigate the effects of hygromycin (Hyg),kanamycin (Kan),cefotaxime (Cef),and timentin (Tim) on callus induction,and the formation of adventitious buds and roots,aiming to screen suitable selective and bacteriostatic antibiotics for the genetic transformation system of yellow calla lily,in order to provide references for determining the types and concentrations of antibiotics in the genetic transformation system of yellow calla lily.The results showed that the sensitivity of‘Jingcai Yangguang’to the four antibiotics ranked as Hyg>Kan>Cef>Tim.The sensitivity of different tissues and organs was in the order of callus > roots > adventitious buds.Based on the formation of callus,adventitious buds,and roots from tuber slices,the effective concentration of kanamycin was 10 times that of hygromycin,while those of cefotaxime and timentin were 100 times that of hygromycin.At 40 mg·L-1 kanamycin,the inhibition rate for callus was 93.76% with a formation rate of 5.65%;for adventitious buds,the inhibition rate was 49.55% with a formation rate of 29.56%.At 10 mg·L-1 hygromycin,the inhibition rate for callus was 97.83% with a formation rate of 1.96%;for adventitious buds,the inhibition rate was 55.38% with a formation rate of 26.15%.Therefore,10 mg·L-1 hygromycin and 40 mg·L-1 kanamycin can be used as selective agents in the genetic transformation system for‘Jingcai Yangguang’using tuber slice explants.Under 300 mg·L-1 cefotaxime, the callus inhibition rate was 80.76% with a formation rate of 17.43%; the adventitious bud inhibition rate was 13.91% with a formation rate of 50.45%. Under 300 mg·L-1 timentin, the callus inhibition rate was 73.18% with a formation rate of 24.29%; the adventitious bud inhibition rate was 16.29% with an formation rate of 49.05%.Thus,300 mg·L-1 timentin or cefotaxime can serve as inhibitors for the growth and proliferation of Agrobacterium in the genetic transformation system.
参考文献/References:
［1］RIBEIRO M D O,PASQUAL M,da SILVA A B,et al.Calla lily in vitro multiplication:Spectrum of light and sucrose［J］.Ciencia Rural,2009,39(8):2388-2394.［2］WEI Z Z,LUO L B,ZHANG H L,et al.Identification and characterization of 43 novel polymorphic EST-SSR markers for Arum lily,Zantedeschia aethiopica (Araceae)［J］.American Journal of Botany,2012,99(12):e493-e497.［3］LETTY C.The genus Zantedeschia［J］.Bothalia,1973,11(1/2):5-26.［4］SINGH,Y,van WYK A E,BAIJNATH H.Know your arums:a comparative guide to the members of the genus Zantedeschia［J］.Veld Flora,1995,81:54-55.［5］SINGH Y,van WYK A E,BAIJNATH H.Taxonomic notes on the genus Zantedeschia Spreng.(Araceae) in southern Africa［J］.South African Journal of Botany,1996,62(6):321-324.［6］PERRY P L.A new species of Zantedeschia (Araceae) from the western Cape［J］.South African Journal of Botany,1989,55(4):447-451.［7］王进忠,高文,高遐虹,等.粉色马蹄莲组织培养研究［J］.北京农学院学报,2005,20(2):10-13.［8］SINGH Y,van WYK A E,BAIJNATH H.Floral biology of Zantedeschia aethiopica (L.) Spreng.(Araceae)［J］.South African Journal of Botany,1996,62(3):146-150.［9］陆方方,邵果园,陈晓梅.黄色马蹄莲的组织培养研究［J］.热带农业科技,2007,30(3):41-42,47.［10］LI L,YUAN L,ZHAO Y,et al.Emergence of bacterial soft rot in Calla lily caused by Pectobacterium aroidearum in China［J］.Crop Protection,2022,152:105854.［11］MARINO B G,GAGGìA F,BAFFONI L,et al.Antimicrobial activity of Melia azedarach fruit extracts for control of bacteria in inoculated in-vitro shoots of ‘MRS 2/5’ plum hybrid and Calla lily and extract influence on the shoot cultures［J］.European Journal of Plant Pathology,2015,141(3):505-521.［12］YAO J L,ROWLAND R E,COHEN D.Interspecific albino and variegated hybrids in the genus Zantedeschia［J］.Plant Science,1995,109(2):199-206.［13］YAO J L,COHEN D,ROWLAND R E.Plastid DNA inheritance and plastome-genome incompatibility in interspecific hybrids of Zantedeschia (Araceae)［J］.Theoretical and Applied Genetics,1994,88(2):255-260.［14］苗双玉,王丽娟.‘圣诞红’草莓再生体系和组培快繁体系的建立［J］.北方园艺,2025(7):9-17.［15］刘蓉,李松,张榉,等.芍药属植物组织培养与遗传转化研究进展［J］.北方园艺,2025(22):104-112.［16］姚冉,石美丽,潘沈元,等.农杆菌介导的植物遗传转化研究进展［J］.生物技术进展,2011,1(4):260-265.［17］李卫,郭光沁,郑锠.根癌农杆菌介导遗传转化研究的若干新进展［J］.科学通报,2000,45(8):798-807.［18］GOODING P.Plant cell-directed control of virion sense gene expression in wheat dwarf virus［J］.Nucleic Acids Research,1999,27(7):1709-1718.［19］朱乾浩.植物转化细胞选择研究进展［J］.世界农业,1996(3):21-24.［20］潘继承,汪劲松.植物基因工程中常用选择标记基因［J］.自然杂志,1993,15(9):25-26.［21］LIN J J,ASSAD-GARCIA N,KUO J.Plant hormone effect of antibiotics on the transformation efficiency of plant tissues by Agrobacterium tumefaciens cells［J］.Plant Science,1995,109(2):171-177.［22］王梅.草莓叶片不定芽发生及抗生素及其影响的研究［D］.雅安:四川农业大学,2005.［23］YIP M K,HUANG H E,GER M J,et al.Production of soft rot resistant Calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants［J］.Plant Cell Reports,2007,26(4):449-457.［24］孙淑玲,冯强,高遐虹,等.抗生素对彩色马蹄莲愈伤组织诱导和生长的影响［J］.河北林业科技,2007(6):1-3.［25］张娇,刘计璇,李也,等.华北绣线菊(Spiraea fritschiana schneid)再生及遗传转化体系建立的研究［J］.植物生理学报,2016,52(12):1851-1860.［26］钟光驰.百合高频再生体系的建立及农杆菌介导基因的转入［D］.重庆:西南大学,2007.［27］李菲,曹永琼,王纲,等.根癌农杆菌介导彩色马蹄莲遗传转化体系的建立及优化［J］.南方农业学报,2024,55(6):1692-1699.［28］李想.彩叶芋‘红桃K’遗传转化体系的优化及AtPAP1基因与玉米Lc基因的导入［D］.重庆:西南大学,2018.［29］贾永芳.半夏离体培养研究及遗传转化的初探［D］.重庆:西南农业大学,2003.［30］温琳.半夏组织培养体系和遗传转化体系的建立［D］.太原:山西大学,2017.［31］王新颖.农杆菌介导法转ipt基因创造半夏新种质［D］.贵阳:贵州大学,2009.［32］孔周阳.农杆菌介导的红掌转抗菌肽基因研究［D］.苏州:苏州大学,2017.［33］牛瑞鹤.红掌遗传转化体系与抗菌肽基因表达载体的构建［D］.苏州:苏州大学,2015.［34］夏鑫,罗燕,蔡艳飞,等.两种月季新品种对根癌农杆菌抗性的初步研究［J］.北方园艺,2025(18):86-94.
备注/Memo
第一作者简介：付娇娇（2000-），女，硕士研究生，研究方向为球根花卉转基因育种。E-mail：fujiaojiao@jib.ac.cn.责任作者：郑玉红（1976-），女，博士，副研究员，现主要从事球根花卉转基因育种及电分析化学在植物学领域中的应用等研究工作。E-mail：zhengyuhong@cnbg.net.基金项目：2025年江苏省研究生实践创新计划资助项目（SJCX25_2629）。收稿日期：2025-12-10