LI Dongkui,WANG Lianxiang,YANG Bin,et al.The Correlation Between Disease Resistance and Sex of Chinese Wild Vitis Species[J].Northern Horticulture,2026,(9):109-116.[doi:10.11937/bfyy.20253681]
中国野生葡萄种内株系间抗病性与性别相关性研究
- Title:
- The Correlation Between Disease Resistance and Sex of Chinese Wild Vitis Species
- 文章编号:
- 1001-0009(2026)09-0109-08
- 分类号:
- S663.1
- 文献标志码:
- A
- 摘要:
- 以中国野生葡萄9个种25个株系为试材,通过田间自然抗病性鉴定、组织结构解剖观察及实时荧光定量PCR技术,系统鉴定分析了其抗白粉病能力,研究了性别差异对白粉病抗性的影响,以期为中国野生葡萄杂交育种提供参考依据。结果表明:白河-35-1♀、江西-1♀、留坝-1♀高抗白粉病。白河-35-2♂、泰山-1♀、泰山-2♂、甘肃91♀高感白粉病;华东葡萄白河-35-1♀与白河-35-2♂叶片组织结构无差异;芪合酶基因STS以及转录因子MYB14、MYB15表达量在华东葡萄白河-35-1♀与白河-35-2♂相对表达量存在着显著差异;中国野生葡萄种内抗病性与性别存在着相关性,这种相关性的本质是受抗病基因决定的。
- Abstract:
- Taking twenty-five strains from nine species of Chinese wild Vitis as test materials,the resistance to powdery mildew was identified and analyzed through field natural disease resistance evaluation,anatomical observation of tissue structure,and quantitative real-time PCR(RT-PCR) technology.Furthermore,the influence of sexual dimorphism on powdery mildew resistance was investigated,in order to provide references for hybrid breeding of Chinese wild Vitis.The results showed that Baihe-35-1 ♀,Jiangxi-1 ♀,and Liuba-1 ♀ exhibited strong resistance to powdery mildew,while Baihe-35-2 ♂,Taishan-1 ♀,Taishan-2 ♂,and Gansu 91 ♀ were highly susceptible.There was no difference in leaf tissue structure between Baihe-35-1 ♀ and Baihe-35-2 ♂ of Vitis pseudoreticulata W.T.Wang.However,significant differences were observed in the relative expression levels of the stilbene synthase gene (STS) and the transcription factors MYB14 and MYB15 between Baihe-35-1 ♀ and Baihe-35-2 ♂.Disease resistance within Chinese wild grape species was correlated with gender,and this correlation was essentially determined by disease-resistant genes.
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备注/Memo
第一作者简介:李东奎(1991-),男,硕士研究生,研究方向为园艺植物和中药材。E-mail:1617735727@qq.com.责任作者:王跃进(1958-),男,博士,教授,博士生导师,现主要从事葡萄种质资源与生物技术等研究工作。E-mail:wangyj@nwsuaf.edu.cn.基金项目:国家自然科学基金面上资助项目(32272667);山东省生态农业技术体系菏泽综合试验站资助项目(SDAIT-30-13)。收稿日期:2025-10-23