CAO Meng,WANG Yong,JIANG Jun,et al.Identification of Key Heat-tolerance Genes in Eggplant Based on Transcriptomics and WGCNA[J].Northern Horticulture,2026,(13):8-18.[doi:10.11937/bfyy.20253676]
基于转录组学和WGCNA挖掘茄子耐热关键基因
- Title:
- Identification of Key Heat-tolerance Genes in Eggplant Based on Transcriptomics and WGCNA
- 文章编号:
- 1001-0009(2026)13-0008-11
- 关键词:
- 茄子; 高温胁迫; 转录组学; 加权基因共表达网络分析
- Keywords:
- eggplant; heat stress; transcriptomics; WGCNA
- 分类号:
- S641.1
- 文献标志码:
- A
- 摘要:
- 以茄子耐热材料‘E94’和热敏材料‘E9’为试材，采用RNA-Seq和WGCNA方法，研究了茄子在42 ℃高温处理2、4、8 h后转录组水平的变化，挖掘茄子响应高温胁迫的重要基因,以期为阐明茄子耐高温机制提供参考依据。结果表明：高温胁迫下共有1 707个基因在耐热中特异性差异表达。富集分析显示，耐热茄子材料在植物-病原体互作、植物激素信号转导、ABC转运蛋白等与热胁迫相关的关键通路方面表现出特异的富集。通过加权共表达网络分析，筛选出29个与高温显著相关的枢纽基因。根据基因表达量和功能注释，进一步锁定热激蛋白HSP26.5（Sme05G1602）和与激素相关的乙烯反应子CRF3（Sme08G2309）、ERF110（Sme05G1987）、脱落酸受体PYL4（Sme05G0273、Sme09G0837、Sme05G0274）、生长素响应基因SAUR32（Sme03G2102）、GH3.1（Sme02G0796）等8个基因为茄子响应高温胁迫的关键候选基因。
- Abstract:
- Taking heat-tolerant eggplant line ‘E94’ and heat-sensitive line ‘E9’ as the test materials,RNA-Seq and WGCNA methods were used to investigate the transcriptomic changes in eggplant after exposure to 42 ℃ for 2,4 and 8 hours,to identify important genes involved in response to high-temperature stress,in order to provide references for clarifying the heat tolerance mechanism of eggplant.The results showed that a total of 1 707 genes were found to be uniquely differentially expressed in the tolerant genotype under heat.Functional enrichment revealed specific over-representation of pathways related to plant-pathogen interaction,plant-hormone signal transduction and ABC transporters,all known to be involved in thermotolerance.WGCNA identified 29 hub genes significantly associated with the heat response.On the basis of expression level and functional annotation,eight genes were further prioritized as key candidates for eggplant heat-stress tolerance,the small heat-shock protein HSP26.5 (Sme05G1602),the ethylene-responsive transcription factors CRF3 (Sme08G2309) and ERF110 (Sme05G1987),the abscisic-acid receptors PYL4 (Sme05G0273,Sme09G0837,Sme05G0274),and the auxin-responsive genes SAUR32 (Sme03G2102) and GH3.1 (Sme02G0796).
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备注/Memo
第一作者简介：曹萌（1998-），女，硕士，研究实习员，现主要从事蔬菜遗传育种等研究工作。E-mail:15130086082@163.com.责任作者：王勇（1986-），男，硕士，副研究员，现主要从事蔬菜遗传育种等研究工作。E-mail:wyong2299@163.com.基金项目：河南省重大科技专项资助项目(241100110200)；国家大宗蔬菜产业技术体系资助项目(CARS-23-G16)；驻马店市重点研发专项资助项目(ZMDSZDYF2025001)。收稿日期：2025-10-22