LI Runyu,JIANG Fan,ZHANG Jiajing,et al.Evaluation of Anthracnose Resistance of Persimmon Germplasm Resources and Correlation Analysis of Physiological Indexes[J].Northern Horticulture,2026,(5):110-118.[doi:10.11937/bfyy.20253332]
柿种质资源炭疽病抗性评价及生理指标相关性分析
- Title:
- Evaluation of Anthracnose Resistance of Persimmon Germplasm Resources and Correlation Analysis of Physiological Indexes
- 文章编号:
- 1001-0009(2026)05-0110-09
- Keywords:
- persimmon; anthracnose; infection; resistance; physical signs
- 分类号:
- S665.2
- 文献标志码:
- A
- 摘要:
- 以7个柿主栽品种为试材，采用田间抗性评价、离体嫩茎侵染及离体叶盘侵染 3 种侵染方法，研究了不同抗性评价方法侵染结果的一致性，品种间炭疽病抗性差异以及品种抗性与可溶性糖含量、木质素含量、过氧化物酶（POD）活性、超氧化物歧化酶（SOD）活性、过氧化氢（H2O2）积累量等生理指标间的相关性，同时以高抗品种‘鸡心黄’和高感品种‘小果甜柿’为材料，研究了水杨酸（SA）、茉莉酸（JA）等激素在不同抗性品种中的含量变化，明确品种抗性差异，筛选关键抗性生理指标，旨在为炭疽病抗性资源筛选及其机制研究提供参考依据。结果表明：不同品种对炭疽病的抗性存在显著差异，‘鸡心黄’表现为高抗，‘垣曲八月红’表现为抗病，‘礼泉山疙瘩’表现为中抗，‘七月鲜’表现为感病，‘富平尖柿’‘曲靖水柿’及‘小果甜柿’表现为高感；柿品种对柿炭疽病的抗性水平与可溶性糖含量、木质素含量及POD、SOD活性呈显著正相关（P<0.05），其中SOD活性与品种抗病性呈极显著正相关（r=0.923 6，P<0.001）；感病前期（0~12 h），SA含量均呈明显上升趋势，且高抗品种SA含量显著高于高感品种，外源SA能显著抑制哈锐炭疽菌（Colletotrichum horii）的生长；离体枝条侵染和叶盘法与田间抗性评价结果基本一致，可作为抗性品种快速鉴定的有效方法；柿树对抗炭疽病侵染的防御过程中，木质素、SA等相关生理指标变化量均与抗病性密切相关，可作为快速筛选和评价的重要指标。
- Abstract:
- Seven main cultivated persimmon varieties were used as experimental materials.Three infection methods，namely field resistance evaluation，in vitro inoculation of tender branches，and in vitro leaf disc infection，were adopted to study the consistency of the infection results of different resistance evaluation methods，the differences in resistance to anthracnose among different varieties，and the correlations between variety resistance and physiological indicators such as soluble sugar content，lignin content，peroxidase (POD) activity，superoxide dismutase (SOD) activity，and hydrogen peroxide (H2O2) accumulation.Meanwhile，using the highly resistant variety ‘Jixinhuang’ and the highly susceptible variety ‘Xiaoguotian Persimmon’ as test materials，the content changes of different hormones such as salicylic acid (SA) and jasmonic acid (JA) in different resistant varieties were studied,to clarify the resistance differences among different main cultivated persimmon varieties to anthracnose，effectively identify the disease resistance of persimmon germplasm resources，screen the key physiological indicators affecting resistance，in order to provide a reference for the screening of anthracnose-resistant resources and the study of its mechanism.The results showed that there were significant differences in the resistance of different varieties to anthracnose.‘Jixinhuang’ showed high resistance，‘Yuanqu Bayuehong’ showed disease resistance，‘Liquanshangeda’ showed moderate resistance，‘Qiyuexian’ showed susceptibility，and ‘Fuping Jian Persimmon’‘Qujing Shui Persimmon’，and ‘Xiaoguotian Persimmon’ showed high susceptibility.The resistance level of varieties to persimmon anthracnose was significantly positively correlated with soluble sugar content，lignin content，and POD，SOD activities (P<0.05).Among them，SOD activity was extremely significantly positively correlated with variety disease resistance (r=0.923 6，P<0.001).In the early stage of infection (0-12 hours)，the SA content showed an obvious upward trend，and the SA content in highly resistant varieties was significantly higher than that in highly susceptible varieties.Exogenous SA could significantly inhibit the growth of Colletotrichum horii.The results of in vitro branch infection and leaf disc methods were basically consistent with the field resistance evaluation results，which could be used as effective methods for the rapid identification of resistant varieties.During the defense process of persimmon trees against anthracnose infection，the changes in physiological indexes related to lignin and SA were closely related to disease resistance and could be used as important indexes for rapid screening and evaluation.
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备注/Memo
第一作者简介：李润雨（2001-），女，硕士研究生，研究方向为果树育种与生物技术。E-mail:1979133144@qq.com.责任作者：关长飞（1987-），男，博士，副教授，博士生导师，现主要从事柿种质资源收集、整理、保存、鉴定及经济形状评价等研究工作。E-mail:guanchangfei@nwafu.edu.cn.基金项目：国家重点研发计划资助项目（2024YFD2200600，2022YFD2200403）；西北农林科技大学渭南试验示范站建设专项资助项目(2025WNXNZX-3)。收稿日期：2025-09-18