[1]刘威,何明,刘石磊,等.甜瓜耐冷资源筛选及耐冷基因CmPYL6和CmPYL7表达分析[J].北方园艺,2025,(24):1-9.[doi:10.11937/bfyy.20252790]
　LIU Wei,HE Ming,LIU Shilei,et al.Evaluation of Cold-tolerant Melon Resources and Expression Profiling of Cold-responsive Genes CmPYL6 and CmPYL7[J].Northern Horticulture,2025,(24):1-9.[doi:10.11937/bfyy.20252790]
点击复制

甜瓜耐冷资源筛选及耐冷基因CmPYL6和CmPYL7表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年24 页码: 1-9 栏目: 出版日期: 2025-12-30
Title:
Evaluation of Cold-tolerant Melon Resources and Expression Profiling of Cold-responsive Genes CmPYL6 and CmPYL7
文章编号:
1001-0009(2025)24-0001-09
作者:
刘威; 何明; 刘石磊; 张丽丽; 吕艳玲; 张家旺
（辽宁省农业科学院 蔬菜研究所，辽宁 沈阳 110161）
Author(s):
LIU Wei; HE Ming; LIU Shilei; ZHANG Lili; LYU Yanling; ZHANG Jiawang
(Vegetable Research Institute，Liaoning Academy of Agricultural Sciences，Shenyang，Liaoning 110161)
关键词:
甜瓜; 冷害胁迫; PYL; ABA受体
Keywords:
melon; cold stress; PYL; ABA receptor
分类号:
S 652
DOI:
10.11937/bfyy.20252790
文献标志码:
A
摘要:
以不同类型薄皮甜瓜为试材，采用冷害胁迫处理、生理指标和实时荧光定量分析等方法，研究了冷害胁迫对甜瓜幼苗形态、胁迫生理指标及CmPYL家族成员表达水平的影响，以期为后续甜瓜耐冷资源快速筛选提供参考依据。结果表明：叶片损伤程度与电解质渗透率呈正相关，可作为评价冷害胁迫损伤等级的判断指标。根据该评价指标，从38份材料中筛选到4份耐冷材料、5份不耐冷材料以及6份中等耐冷材料。耐冷试材No.10经4 ℃冷害胁迫后的存活率可达到90%以上，而不耐冷试材No.28的存活率仅12%。此外，耐冷试材No.10体内脯氨酸和可溶性糖含量均高于不耐冷试材No.28。CmPYLs家族成员表达分析发现，经冷害胁迫处理后，CmPYL6和CmPYL7在耐冷试材No.4和No.10体内的表达水平升高200倍以上，在中等耐冷试材No.9和No.19体内升高100倍以上，而在不耐冷试材No.7和No.28体内不受诱导甚至受到抑制，说明CmPYL6和CmPYL7与甜瓜耐冷能力正相关。
Abstract:
Taking different types of oriental melon as the test materials，the effects of cold injury stress on the morphology，stress physiological indicators，and expression levels of CmPYL family members in melon seedlings were studied by using methods such as cold injury stress treatment，physiological indicators，and real-time fluorescence quantitative analysis,in order to provide reference for rapid screening of cold tolerant resources in cantaloupe in the future.The results showed that these parameters were effective indicators for evaluating cold stress responses，thereby establishing a benchmark for selecting cold-tolerant varieties.Based on this criterion，38 materials were subjected to cold stress and identified 4 cold-tolerant materials，5 cold-sensitive materials，and 6 moderately cold-tolerant materials.The cold-tolerant material No.10 exhibited a survival rate exceeding 90% after 4 ℃ cold treatment，whereas the cold-sensitive material No.28 had a survival rate of only 12%.Additionally，the proline and soluble sugar contents in cold-tolerant No.10 were higher than those in cold-sensitive No.28.Expression analysis of CmPYLs family members revealed that after cold treatment，CmPYL6 and CmPYL7 expression levels increased more than 200-fold in cold-tolerant materials No.4 and No.10，over 100-fold in moderately cold-tolerant materials No.9 and No.19，but were either uninduced or suppressed in cold-sensitive materials No.7 and No.28.This indicated that CmPYL6 and CmPYL7 were positively correlated with melon cold tolerance.

参考文献/References:

［1］蒋先明.蔬菜栽培学总论［M］.北京:中国农业出版社，2000.［2］程智慧.蔬菜栽培学各论［M］.北京:科学出版社，2010.［3］ZHU J K.Abiotic Stress signaling and responses in plants［J］.Cell，2016，167(2)：313-324.［4］PARK S Y，FUNG P，NISHIMURA N，et al.Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins［J］.Science，2009(324)：1068-1071.［5］MA Y，SZOSTKIEWICZ I，KORTE A，et al.Regulators of PP2C phosphatase activity function as abscisic acid sensors［J］.Science，2009(324)：1064-1068.［6］QIAN Z，HE L，LI F.Understanding cold stress response mechanisms in plants：an overview［J］.Frontiers in Plant Science，2024(15)：1443317.［7］纪环宇,刘晓嘉,唐雪东,等.外源ABA对低温胁迫下越橘花期和幼果期抗寒性的影响［J］.北方园艺，2023(1):27-33.［8］HOU H，LV L，HUO H，et al.Genome-wide identification of the ABA receptors genes and their response to abiotic stress in apple［J］.Plants，2020(9)：1028.［9］LENKA S K，MUTHUSAMY S K，CHINNUSAMY V，et al.Ectopic Expression of rice PYL3 enhances cold and drought tolerance in Arabidopsis thaliana［J］.Molecular Biotechnology，2018(60)：350-361.［10］ZHANG Q，KONG X，YU Q，et al.Responses of PYR/PYL/RCAR ABA receptors to contrasting stresses，heat and cold in Arabidopsis［J］.Plant Signaling & Behavior，2019(14)：1670596.［11］LIU W，LV Y，ZHANG L，et al.CmPYL6 contributes greatly to cold tolerance in oriental melon［J］.Plant Biology，2024，26(6)：1033-1046.［12］LIU W，JIANG Y，LV Y，et al.CmPYL7 positively regulates the cold tolerance via interacting with CmPP2C24-like in oriental melon［J］.Physiologia Plantarum，2024，176(6)：e14628.［13］LI Y，ZHAO H，DUAN B，et al.Effect of drought and ABA on growth，photosynthesis and antioxidant system of Cotinus coggygria seedlings under two different light conditions［J］.Environmental and Experimental Botany，2011(71)：107-113.［14］BATES L S，WALDREN R P，TEARE I D.Rapid determination of free proline for water-stress studies［J］.Plant and Soil，1973(39)：205-207.［15］DU Y，ZHAO Q，CHEN L，et al.Effect of drought stress on sugar metabolism in leaves and roots of soybean seedlings［J］.Plant Physiology and Biochemistry，2020(146)：1-12.［16］LIU W，JIN Y，LI M，et al.Analysis of CmCADs and three lignifying enzymes in oriental melon (‘CaiHong7’) seedlings in response to three abiotic stresses［J］.Scientia Horticultare,2018(237)：257-268.［17］JIN Y，ZHANG C，LIU W，et al.The cinnamyl alcohol dehydrogenase gene family in melon (Cucumis melo L.)：Bioinformatic analysis and expression patterns［J］.PLoS One，2014，9(7)：e101730.［18］LYONS J M.Chilling injury in plants［J］.Annual Review of Plant Biology，1973(24)：445-466.［19］LUKATKIN A S，BRAZAITYTE A，BOBINAS C，et al.Chilling injury in chilling-sensitive plants：A review［J］.Agriculture，2012，99(2)：111-124.［20］李薇，史菲，刘敏，陈水森.植物响应低温的生理和分子机制研究进展［J］.北方园艺，2023(8):121-126.［21］CAMPOS P S，QUARTIN V，RAMALHO J C，et al.Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp.plants［J］.Plant Physiology，2003(160)：283-292.［22］SZABADOS L，SAVOUR A.Proline：A multifunctional amino acid［J］.Trends in Plant Science，2010,15(2)：89-97.［23］MA Y，ZHANG Y，LU J，et al.Roles of plant soluble sugars and their responses to plant cold stress［J］.African Journal of Biotechnology，2009，8(10)：2004-2010.［24］KUMAR V，YADAV S K.Proline and betaine provide protection to antioxidant and methylglyoxal detoxification systems during cold stress in Camellia sinensis (L.) O.Kuntze［J］.Acta Physiologiae Plantarum,2009(31)：261-269.［25］LI M，YUE T，HAN J，et al.Exogenous glucose irrigation alleviates cold stress by regulating soluble sugars，ABA and photosynthesis in melon seedlings［J］.Plant Physiology and Biochemistry，2024(217)：109214.［26］ZHAO Y，QI G，REN F，et al.Analysis of PYL genes and their potential relevance to stress tolerance and berry ripening in grape［J］.Journal of the American Society for Horticultural Science，2020(145)：308-317.

相似文献/References:

[1]王晓静,马文平,王芳.甜瓜采后致病菌交链菌的防治方法研究[J].北方园艺,2014,38(04):103.
　WANG Xiao-jing,MA Wen-ping,WANG Fang.Study on Prevention Method of Alternariasp. After Melon Postharvest[J].Northern Horticulture,2014,38(24):103.
[2]宁雪飞,韩小路,王贤磊,等.一种快速筛选转抗霜霉病基因甜瓜种子的方法研究[J].北方园艺,2014,38(01):102.
　NING Xue-fei,HAN Xiao-lu,WANG Xian-lei,et al.The Screening of Transgenic Plants Resistant to Downy Mildew Disease in Melon[J].Northern Horticulture,2014,38(24):102.
[3]王晓梅,刘国宁,程丽,等.部分黄瓜和甜瓜品种对霜霉病的抗性及其组分研究[J].北方园艺,2014,38(04):106.
　WANG Xiao-mei,LIU Guo-ning,CHENG Li,et al.Study on Resistance to Downy Mildew of Some Cucumber and Melon Varieties and Its Component[J].Northern Horticulture,2014,38(24):106.
[4]张君艳,罗爱玉,张君君.河西地区杂交甜瓜大田制种生产技术[J].北方园艺,2013,37(14):62.
　[J].Northern Horticulture,2013,37(24):62.
[5]苏艳,王盼乔,胡建斌.十五份甜瓜种质苗期性状的聚类分析及相关分析[J].北方园艺,2014,38(07):5.
　SU Yan,WANG Pan-qiao,HU Jian-bin.Clustering and Correlation Analysis of 15 Cucumis melo L.Germplasms Based on the Phenotypic Traits of the Seedling Period[J].Northern Horticulture,2014,38(24):5.
[6]赵九洲,胡立盼,徐志然,等.甜瓜幼苗耐盐碱性及缓解盐碱胁迫γ-氨基丁酸浓度的筛选[J].北方园艺,2014,38(09):1.
　ZHAO Jiu-zhou,HU Li-pan,XU Zhi-ran,et al.The Screening of Melon（Cucumis melo L.）Seedling Cultivars for Salt-alkaline Tolerance and the Concentration of γ-aminobutyric Acid Alleviating Salt-alkaline Stress[J].Northern Horticulture,2014,38(24):1.
[7]王喜涛,周秀艳,辛 明,等.盐胁迫对甜瓜种子发芽的影响[J].北方园艺,2014,38(09):7.
　WANG Xi-tao,ZHOU Xiu-yan,XIN Ming,et al.Effect of NaCl Stress on Seed Germination of Melon[J].Northern Horticulture,2014,38(24):7.
[8]宋 羽,卢青成,王 浩.适合新疆戈壁地区甜瓜穴盘育苗的基质相对含水量的探究[J].北方园艺,2014,38(09):53.
　SONG Yu,LU Qing-cheng,WANG Hao.Research on Suitable Substrate Water Content for Melon Plug-seedling in Gobi District of Xinjiang[J].Northern Horticulture,2014,38(24):53.
[9]苏丽影,宋述尧,赵春波,等.玉米秸秆混合基质对甜瓜穴盘苗生长的影响[J].北方园艺,2013,37(12):28.
　SU Li-ying,SONG Shu-yao,ZHAO Chun-bo,et al.Effect of Mixed Substrates of Corn Stalk on Plug Seeding of Muskmelon[J].Northern Horticulture,2013,37(24):28.
[10]王爱玲,张敏,廖新福,等.甜瓜“黄醉仙”下胚轴再生体系的初步建立[J].北方园艺,2013,37(12):100.
　WANG Ai-ling,ZHANG Min,LIAO Xin-fu,et al.Preliminary Establishment of Melon cv.‘Huangzuixian’ Regeneration System[J].Northern Horticulture,2013,37(24):100.

备注/Memo

第一作者简介：刘威（1989-），男，博士，助理研究员，现主要从事甜瓜低温胁迫生理等研究工作。E-mail：qinghuishuimu@126.com.责任作者：张家旺（1980-），男，硕士，副研究员，现主要从事甜瓜品种选育等研究工作。E-mail：zhangjiawang1020@163.com.基金项目：辽宁省农业科学院优秀青年基金资助项目（2025YQ0202）；国家现代农业产业技术体系资助项目（CARS-25）。收稿日期：2025-07-31

更新日期/Last Update: 2026-01-09