GUO Jinghua,WANG Fuhao,HAO Peipei,et al.Effects of N-phenyl-phthalamic Acid on Rapeseed Growth Under ZJ0272 Stress[J].Northern Horticulture,2025,(20):49-59.[doi:10.11937/bfyy.20251388]
异丙酯草醚对油菜的药害以及苯肽胺酸缓解机制的研究
- Title:
- Effects of N-phenyl-phthalamic Acid on Rapeseed Growth Under ZJ0272 Stress
- 文章编号:
- 1001-0009(2025)20-0049-11
- Keywords:
- ZJ0272; N-phenyl-phthalamic acid; physiological and biochemical indicators; herbicide stress; gene expression
- 分类号:
- S 565.4
- 文献标志码:
- A
- 摘要:
- 以白菜型油菜“华油100”为试材，采用形态学观察、显微结构分析及生理生化指标测定以及抗氧化基因表达量分析等方法，研究了除草剂异丙酯草醚（ZJ0272）和苯肽胺酸（PA）对油菜的药害及缓解效应，以期为油菜田安全除草及生长调节剂应用提供参考依据。结果表明：异丙酯草醚浓度＞0.5 g·L-1时显著抑制油菜生长，叶片黄化卷曲明显，茎筛管组织损伤加剧；喷施1.0 g·L-1 PA后，0.1~0.5 g·L-1异丙酯草醚处理的药害症状明显缓解，叶片黄化减弱，茎表层细胞有一定程度的恢复。生理分析表明，在施药后7 d内，随着异丙酯草醚浓度升高，其叶片的丙二醛（MDA）含量逐渐升高，过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）活性和叶绿素、谷胱甘肽（GSH）含量呈先升高后降低的趋势；喷施苯肽胺酸缓解后，异丙酯草醚浓度为0.1、0.2 g·L-1和0.5 g·L-1时，油菜药害现象缓解明显，MDA含量降低，CAT、POD、SOD活性显著升高，叶绿素、GSH含量升高；异丙酯草醚浓度为1.0 g·L-1时，喷施苯肽胺酸无明显缓解效果。表明PA通过激活抗氧化系统及调控基因表达缓解异丙酯草醚胁迫，对0.5 g·L-1以下浓度药害缓解效果显著。
- Abstract:
- Taking Brassica napus ‘Huayou 100’ as test material,morphological observation，microstructure analysis，physiological and biochemical index determination and antioxidant gene expression analysis were used，the phytotoxicity and mitigation effects of ZJ0272 and PA on rapeseed were studied，in order to provide reference for safe weeding and growth regulator application in rapeseed fields.The results showed that when the concentration of ZJ0272 was more than 0.5 g·L-1，the growth of rapeseed was significantly inhibited，the yellowing and curling of leaves were obvious，and the damage of stem sieve tube tissue was aggravated.After spraying 1.0 g·L-1 PA，the phytotoxicity symptoms of 0.1-0.5 g·L-1 ZJ0272 treatments were significantly alleviated，the yellowing of leaves was weakened，and the stem surface cells recovered to a certain extent.Physiological analysis showed that the content of MDA in leaves increased gradually with the increase of ZJ0272 concentration within 7 days after application，and the activities of CAT，POD，SOD and the contents of chlorophyll and GSH increased first and then decreased.After spraying PA，when the concentration of ZJ0272 was 0.1 g·L-1，0.2 g·L-1 and 0.5 g·L-1，the phytotoxicity of rapeseedwas significantly alleviated，the content of MDA decreased，activities of CAT，POD and SOD increased significantly，and the content of chlorophyll and GSH increased.When the concentration of ZJ0272 was 1.0 g·L-1，spraying PA had no obvious mitigation effect.The results showed that PA alleviated the stress of ZJ0272 by activating the antioxidant system and regulating gene expression，and the effect of alleviating the phytotoxicity of 0.5 g·L-1 or less was significant，which provided theoretical support for safe weeding and growth regulator application in rapeseed fields.
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备注/Memo
第一作者简介：郭婧华（1997-），女，硕士研究生，研究方向为除草剂药害以及缓解。E-mail:1003884390@qq.com.责任作者：程有普(1969-），男，博士，教授，现主要从事农药残留与环境行为等研究工作。E-mail:chengyoupu@tjau.edu.cn.基金项目：国家自然科学基金资助项目（31572034）；天津市企业科技特派员资助项目（19JCTPJC59000）。收稿日期：2025-04-13