LIN Rongyan,KONG Lan,LIN Bing,et al.Analysis of Phenotypic Traits and SRAP Molecular Markers in Hybrid Progenies of Cymbidium goeringii[J].Northern Horticulture,2025,(20):23-30.[doi:10.11937/bfyy.20251278]
春兰杂交后代的表型性状及SRAP分子标记分析
- Title:
- Analysis of Phenotypic Traits and SRAP Molecular Markers in Hybrid Progenies of Cymbidium goeringii
- 文章编号:
- 1001-0009(2025)20-0023-08
- 分类号:
- S 682.31
- 文献标志码:
- A
- 摘要:
- 以春兰‘黄荷梅’‘黄荷’及其18个杂交后代株系为试材，采用表型性状观测结合SRAP分子标记鉴定的方法，对表型性状进行变异系数、多样性指数、主成分分析，利用筛选的SRAP分子标记引物组合进行杂交后代真实性和遗传多样性分析，并分别基于表型性状和SRAP数据进行聚类分析，以期为春兰新品种选育提供参考依据。结果表明：20个数量性状变异系数为 8.43%~37.41%，花序长度、花朵数和植株高度变异幅度较大；8个质量性状的信息多样性指数为0.47~1.57，遗传多样性指数为0.18~0.66，花型、花姿态、萼片形状和唇瓣姿态多样性较为丰富；共选出8个主成分，可以代表表型性状89.914%的遗传信息；基于表型性状的聚类分析将杂交后代分为2个类群，其中14个杂交后代株系与母本聚为一类；其他4个杂交后代株系与父本聚为一类。根据6对SRAP引物组合的父本特征带扩增情况，鉴定出18个杂交后代为真杂种；6对SRAP引物组合的平均多态性比例为80.43%，多态性信息含量均值为0.81；基于SRAP数据的聚类分析在遗传距离为0.34时将杂交后代分为三大类群，77.78%的杂交后代在亲缘关系上与母本聚为一类。综上所述，供试的‘黄荷梅’ב黄荷’杂交后代均为真杂种，其表型性状具有丰富的遗传多样性，且表现为偏母本遗传。
- Abstract:
- Taking Cymbidium goeringii ‘Huanghemei’‘Huanghe’ and their 18 hybrid offspring lines as the test materials,the method of phenotype observation combined with SRAP molecular marker identification was used to analyze the coefficient of variation,diversity index,and principal component of phenotype traits.The screened SRAP molecular marker primer combination was used to analyze the authenticity and genetic diversity of hybrid offspring,and cluster analysis was conducted based on phenotype traits and SRAP data,in order to provide reference for the breeding of new varieties of Cymbidium goeringii.The results showed that the variation coefficients of 20 quantitative characters were from 8.43% to 37.41%，and there were more abundant variation in inflorescence length，flower number and plant height.The information diversity index of 8 quality traits ranged from 0.47 to 1.57，and the genetic diversity index ranged from 0.18 to 0.66，among which flower type，flower posture，sepal shape and labellum posture were abundant.8 principal components could represent 89.914% of genetic information of phenotypic traits.Divided into two groups based on phenotypic characteristics，14 hybrid progenies clustered with the maternal parent，while the remaining 4 hybrid progenies grouped with the paternal parent.18 hybrid progenies were identified as true hybrids based on the amplification of 6 pairs of SRAP primer combinations.The polymorphic ratio of the 6 pairs of SRAP primer combinations was 80.43%，and the average of polymorphism information content was 0.81.Based on SRAP data,the hybrid progenies were divided into three groups with a genetic distance of 0.34，77.78% of the hybrid offspring were clustered with the maternal parent in terms of parentage.In conclusion，the tested hybrid progenies were all true hybrids，their phenotypic traits exhibited substantial genetic diversity and demonstrated a maternal inheritance bias.
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备注/Memo
第一作者简介：林榕燕（1990-），女，硕士，助理研究员，现主要从事花卉生物技术等研究工作。E-mail：lryyan@163.com.责任作者：钟淮钦（1979-），男，硕士，研究员，现主要从事观赏植物种质资源评价与创新利用等研究工作。E-mail：zhqeast@163.com.基金项目：福建省科技计划公益类资助项目（2022R1031005）；福建省自然科学基金资助项目（2023J01199）；福建省农业科学院英才资助项目（YC2021013）；福建省农业科学院科技创新团队建设资助项目（CXTD2021010-2）；福建省人民政府-中国农业科学院“5511”协同创新工程资助项目（XTCXGC2021016）。收稿日期：2025-04-03