SUN Shuqi,ZOU Zhiyong,LIU Xiaohan,et al.Genome-wide Identification，Characterization，and Expression Profiling of the WRKY Gene Family in Mesembryanthemum crystallinum[J].Northern Horticulture,2025,(20):31-41.[doi:10.11937/bfyy.20250931]
冰菜WRKY基因家族的全基因组鉴定、功能特征及表达模式分析
- Title:
- Genome-wide Identification，Characterization，and Expression Profiling of the WRKY Gene Family in Mesembryanthemum crystallinum
- 文章编号:
- 1001-0009(2025)20-0031-11
- Keywords:
- Mesembryanthemum crystallinum; WRKY gene family; gene family identification; bioinformatics analysis; expression analysis
- 分类号:
- S 649
- 文献标志码:
- A
- 摘要:
- 以耐盐植物冰菜（Mesembryanthemum crystallinum）为试材，基于全基因组数据整合生物信息学分析，系统鉴定出47个McWRKY基因家族成员，以期为作物耐盐遗传改良提供参考依据。结果表明：该家族基因编码蛋白长度为147~1 196 aa，等电点4.92~9.88，系统发育分析将其划分为Group Ⅰ~Ⅲ三大进化类群，其中Group Ⅱ可进一步细分为5个亚组。染色体定位显示基因成员不均匀分布于9条染色体，42个基因具有内含子结构。启动子区预测到1 527个胁迫响应相关顺式作用元件（ABRE、MYB、W-box等），共线性分析检测到14对片段复制事件。梯度盐胁迫（0~500 mmol·L-1 NaCl）下转录组数据揭示7个McWRKY基因呈现剂量依赖性表达上调。McWRKY基因通过结构多样性、顺式元件富集及动态表达调控参与冰菜耐盐性形成，其中McWRKY22、McWRKY35等WRKY转录因子可能作为核心调控因子激活耐盐相关信号通路。
- Abstract:
- Taking halophyte Mesembryanthemum crystallinum as the test materials，47 McWRKY transcription factor genes were systematically identified through whole-genome data and bioinformatics analysis，in order to provide reference for genetic improvement of crop salt tolerance.The results showed that the gene encoding proteins range from 147 to 1 196 amino acids (aa) with isoelectric points of 4.92-9.88.Phylogenetic analysis classified these genes into three major evolutionary groups (Group Ⅰ-Ⅲ)，with Group Ⅱ further subdivided into five subgroups.Chromosomal mapping revealed their uneven distribution across nine chromosomes，and 42 genes contained intronic structures.Promoter analysis identified 1 527 stress-responsive cis-acting elements (e.g.，ABRE，MYB，and W-box)，while collinearity analysis detected 14 segmental duplication events.Transcriptomic profiling under gradient salt stress (0-500 mmol·L-1 NaCl) demonstrated dose-dependent upregulation of seven McWRKY genes.These findings suggest that structural diversity，cis-element enrichment，and dynamic expression regulation of McWRKY genes contribute to salt tolerance in M.crystallinum，with McWRKY22，McWRKY35 potentially acting as core regulators to activate salt-responsive signaling pathways.
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备注/Memo
第一作者简介：孙姝琦（1998-），女，硕士研究生，研究方向为冰菜耐盐机制。E-mail：zzksbx@163.com.责任作者：李楠（1989-），女，博士，讲师，现主要从事环境调控蔬菜品质的形成及冰菜耐盐机制等研究工作。E-mail：392016474@qq.com.基金项目：河北省自然科学基金青年基金资助项目（C2021209019）；唐山市基础研究科技计划资助项目（22130231H）。收稿日期：2025-03-14