ZENG Yukang,LI Rongchun.Frontier Methods and Future Prospects of Edible Fungus Biological Breeding Technology[J].Northern Horticulture,2025,(17):116-122.[doi:10.11937/bfyy.20250235]
食用菌生物育种技术前沿方法及其未来展望
- Title:
- Frontier Methods and Future Prospects of Edible Fungus Biological Breeding Technology
- 文章编号:
- 1001-0009(2025)17-0116-07
- Keywords:
- edible fungi; biological breeding technology; prospect
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 生物育种技术作为一种新兴手段,为传统育种注入了新的活力与潜力。食用菌是一类拥有大型子实体的真菌,具备食用或药用价值,其营养价值极为丰富。然而,随着食用菌市场的迅猛扩张,对高品质菌株的需求日益提升。但受限于食用菌复杂的遗传特性及传统育种方法的低效性,选育出高产优质的品种面临重重挑战,亟需解决这一问题。该研究旨在介绍生物育种技术,涵盖分子标记辅助育种、基因编辑、全基因组选择等前沿方法,并展望食用菌生物育种领域的未来发展前景。
- Abstract:
- As an emerging approach,biotechnology in breeding has infused new vitality and potential into traditional breeding methods.Edible mushrooms,a class of fungi characterized by their large fruiting bodies,possessing both culinary and medicinal properties,boasting an exceptionally rich nutritional profile.Nevertheless,amidst the rapid expansion of the edible mushroom market,the demand for high-quality strains has escalated significantly.However,hindered by the intricate genetic traits of edible mushrooms and the inefficiency of traditional breeding techniques,the cultivation of high-yielding and superior-quality varieties poses formidable challenges that necessitate urgent resolution.This study aims to introduce biotechnology in edible mushroom breeding,encompassing cutting-edge methodologies such as molecular marker-assisted selection,gene editing,and genome-wide selection,while also offering insights into the future development prospects of this burgeoning field.
参考文献/References:
[1]ZHANG Y,WANG D,CHEN Y,et al.Healthy function and high valued utilization of edible fungi[J].Food Science and Human Wellness,2021,10(4):408-420.[2]李玉.中国食用菌产业发展现状、机遇和挑战:走中国特色菇业发展之路,实现食用菌产业强国之梦[J].菌物研究,2018,16(3):125-131.[3]张俊飚,李鹏.我国食用菌新兴产业发展的战略思考与对策建议[J].华中农业大学学报(社会科学版),2014(5):1-7.[4]鲍大鹏,邹根,裴晓东,等.中国食用菌产业实现高质量现代化发展的路径探讨[J].食用菌学报,2022,29(6):103-110.[5]钟小云,李钦艳.野生食用菌的人工驯化栽培[J].食药用菌,2019,27(3):174-176.[6]王艳.灰树花杂交选育及其苯丙氨酸解氨酶基因的功能研究[D].济南:山东师范大学,2020.[7]汤静.食用菌栽培技术研究[J].种子科技,2022,40(5):46-48.[8]李亚娇,孙国琴,郭九峰,等.原生质体融合技术在食用菌上应用研究进展[J].北方农业学报,2021,49(6):121-127.[9]THONGBAI B,WITTSTEIN K,RICHTER C,et al.Successful cultivation of a valuable wild strain of Lepista sordida from Thailand[J].Mycological Progress,2017,16(4):311-323.[10]史红鸽,魏银初,班新河,等.平菇新品种“驻研1号”的选育[J].北方园艺,2021(8):177-180.[11]张美彦,于海龙,章炉军,等.香菇‘申香1644’的选育报告[J].菌物学报,2022,41(7):1134-1136.[12]赵辉,孙传齐,徐德,等.花脸香蘑新品种‘达香蘑1号’[J].园艺学报,2021,48(S2):2859-2860.[13]张于,邳植,李胜男,等.甜菜生物育种的研究进展及未来展望[J].中国农学通报,2023,39(29):8-13.[14]李月明,毕延震.猪生物育种研究进展[J].湖北农业科学,2022,61(21):115-119.[15]郭锐,钱前,高振宇.水稻生物育种研究进展[J].中国基础科学,2022,24(4):9-17.[16]吴磊,杨涛,陈红霖,等.食用豆生物育种研究进展[J].中国基础科学,2022,24(4):53-64,68.[17]李洪杰,陈明,李少雅,等.小麦生物育种:进展、机遇和挑战[J].中国基础科学,2022,24(4):1-8,28.[18]胡江,钱前.作物生物育种科技发展的现状及展望[J].中国基础科学,2022,24(6):1-8.[19]邢生炎,黄永震,吕世杰,等.生物育种技术及其在畜禽育种中的应用研究进展[J].中国畜牧杂志,2024,60(3):57-65.[20]黎裕,徐辰武,秦峰,等.玉米生物育种:现状与展望[J].中国基础科学,2022,24(4):18-28.[21]WALLACE J G,RODGERS-MELNICK E,BUCKLER E S.On the road to breeding 4.0:Unraveling the good,the bad,and the boring of crop quantitative genomics[J].Annual Review of Genetics,2018,52:421-444.[22]LANDE R,THOMPSON R.Efficiency of marker-assisted selection in the improvement of quantitative traits[J].Genetics,1990,124(3):743-756.[23]FERNANDO R L,GROSSMAN M.Marker assisted selection using best linear unbiased prediction[J].Genetics Selection Evolution,1989,21(4):467-477.[24]高安礼,何华纲,陈全战,等.分子标记辅助选择小麦抗白粉病基因〖STBX〗Pm2、Pm4a和Pm21〖STBZ〗的聚合体[J].作物学报,2005,31(11):16-21.[25]邓化冰,邓启云,陈立云,等.马来西亚普通野生稻增产QTL的分子标记辅助选择及其育种效果[J].中国水稻科学,2007,21(6):605-611.[26]李莉,邢跃先,赵贤容,等.利用分子标记辅助选择技术提高玉米对丝黑穗病的抗性[J].植物保护学报,2012,39(4):303-307.[27]SCHMIERER D A,KANDEMIR N,KUDRNA D A,et al.Molecular marker-assisted selection for enhanced yield in malting barley[J].Molecular Breeding,2004,14(4):463-473.[28]WANG C,CHEN S,YU S.Functional markers developed from multiple loci in GS3 for fine marker-assisted selection of grain length in rice[J].Theoretical and Applied Genetics,2011,122(5):905-913.[29]KERRIGAN R W,ROYER J C,BALLER L M,et al.Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus[J].Genetics,1993,133(2):225-236.[30]LARRAYA L M,PREZ G,RITTER E,et al.Genetic linkage map of the edible basidiomycete Pleurotus ostreatus[J].Applied and Environmental Microbiology,2000,66(12):5290-5300.[31]KWAN H S,XU H L.Construction of a genetic linkage map of shiitake mushroom Lentinula edodes strain L-54[J].Journal of Biochemistry and Molecular Biology,2002,35(5):465-471.[32]高巍.金针菇SCAR标记遗传连锁图的构建[D].福州:福建农林大学,2007.[33]孔庆芳.草菇SCAR标记遗传连锁群的建立[D].福州:福建农林大学,2010.[34]陈志娥.斑玉蕈SCAR标记遗传连锁图的构建[D].福州:福建农林大学,2013.[35]IM C H,PARK Y H,HAMMEL K E,et al.Construction of a genetic linkage map and analysis of quantitative trait loci associated with the agronomically important traits of Pleurotus eryngii[J].Fungal Genetics and Biology,2016,92:50-64.[36]GONG W B,LI L,ZHOU Y,et al.Genetic dissection of fruiting body-related traits using quantitative trait loci mapping in Lentinula edodes[J].Applied Microbiology and Biotechnology,2016,100(12):5437-5452.[37]LU L X,YAO F J,WANG P,et al.Construction of a genetic linkage map and QTL mapping of agronomic traits in Auricularia auricula-judae[J].Journal of Microbiology,2017,55(10):792-799.[38]JASIN M.Genetic manipulation of genomes with rare-cutting endonucleases[J].Trends in Genetics,1996,12(6):224-228.[39]JOUNG J K,SANDER J D.TALENs:A widely applicable technology for targeted genome editing[J].Nature Reviews Molecular Cell Biology,2012,14(1):49-55.[40]WOOD A J,LO T W,ZEITLER B,et al.Targeted genome editing across species using ZFNs and TALENs[J].Science,2011,333(6040):307.[41]DOUDNA J A,CHARPENTIER E.Genome editing.The new frontier of genome engineering with CRISPR-Cas9[J].Science,2014,346(6213):1258096.[42]WALTZ E.Gene-edited CRISPR mushroom escapes US regulation[J].Nature,2016,532(7599):293.[43]SUGANO S S,SUZUKI H,SHIMOKITA E,et al.Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea,optimized by a high-throughput transformation system[J].Scientific Reports,2017,7(1):1260.[44]LIU J,CUI H,WANG R,et al.A simple and efficient CRISPR/Cas9 system using a ribonucleoprotein method for Flammulina filiformis[J].Journal of Fungi,2022,8(10):1000.[45]WANG P A,XIAO H,ZHONG J J.CRISPR-Cas9 assisted functional gene editing in the mushroom Ganoderma lucidum[J].Applied Microbiology and Biotechnology,2020,104(4):1661-1671.[46]BOONTAWON T,NAKAZAWA T,HORII M,et al.Functional analyses of Pleurotus ostreatus pcc1 and clp1 using CRISPR/Cas9[J].Fungal Genetics and Biology,2021,154:103599.[47]MEUWISSEN T H,HAYES B J,GODDARD M E.Prediction of total genetic value using genome-wide dense marker maps[J].Genetics,2001,157(4):1819-1829.[48]HU J,CHEN B,ZHAO J,et al.Genomic selection and genetic architecture of agronomic traits during modern rapeseed breeding[J].Nature Genetics,2022,54(5):694-704.[49]SUN B,GUO R,LIU Z,et al.Genetic variation and marker-trait association affect the genomic selection prediction accuracy of soybean protein and oil content[J].Frontiers in Plant Science,2022(13):1064623.[50]YIN C,ZHOU P,WANG Y,et al.Using genomic selection to improve the accuracy of genomic prediction for multi-populations in pigs[J].Animal,2024,18(2):101062.[51]ISLAM M S,MCCORD P H,OLATOYE M O,et al.Experimental evaluation of genomic selection prediction for rust resistance in sugarcane[J].The Plant Genome,2021,14(3):e20148.[52]CUI Y,LI R,LI G,et al.Hybrid breeding of rice via genomic selection[J].Plant Biotechnology Journal,2020,18(1):57-67.[53]BEYENE Y,SEMAGN K,MUGO S,et al.Genetic gains in grain yield through genomic selection in eight bi-parental maize populations under drought stress[J].Crop Science,2015,55(1):154-163.[54]XU Y,WANG X,DING X,et al.Genomic selection of agronomic traits in hybrid rice using an NCII population[J].Rice,2018,11(1):32.[55]DELLA COLETTA R,FERNANDES S B,MONNAHAN P J,et al.Importance of genetic architecture in marker selection decisions for genomic prediction[J].Theoretical and Applied Genetics,2023,136(11):220.[56]REN J,LI Z,WU P,et al.Genetic dissection of quantitative resistance to common rust (Puccinia sorghi) in tropical maize (Zea mays L.) by combined genome-wide association study,linkage mapping,and genomic prediction[J].Frontiers in Plant Science,2021(12):692205.[57]ZHAO Y,LI Z,LIU G,et al.Genome-based establishment of a high-yielding heterotic pattern for hybrid wheat breeding[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(51):15624-15629.[58]STEWART-BROWN B B,SONG Q,VAUGHN J N,et al.Genomic selection for yield and seed composition traits within an applied soybean breeding program[J].G3:Genes,Genomes,Genetics,2019,9(7):2253-2265.[59]FERNANDES S B,DIAS K O G,FERREIRA D F,et al.Efficiency of multi-trait,indirect,and trait-assisted genomic selection for improvement of biomass sorghum[J].Theoretical and Applied Genetics,2018,131(3):747-755.[60]SWEENEY D W,ROONEY T E,SORRELLS M E.Gain from genomic selection for a selection index in two-row spring barley[J].The Plant Genome,2021,14(3):e20138.[61]鲍大鹏,谭琦.我国食用菌产业发展面临的重大科学问题和技术问题[J].食药用菌,2024,32(4):217-225.[62]GAO W,QU J,ZHANG J,et al.A genetic linkage map of Pleurotus tuoliensis integrated with physical mapping of the de novo sequenced genome and the mating type loci[J].BMC Genomics,2018,19(1):18.[63]GAO W,WEIJN A,BAARS J J P,et al.Quantitative trait locus mapping for bruising sensitivity and cap color of Agaricus bisporus (button mushrooms)[J].Fungal Genetics and Biology,2015,77:69-81.[64]沈真辉,罗祥英,赵仙伟,等.利用转录组分析光照对金耳子实体转色的影响[J].食用菌学报,2023,30(6):12-27.[65]张妍,黄晨阳,高巍.食用菌分子育种研究进展[J].菌物研究,2019,17(4):229-239.[66]刘静,王伟霞,李福后.多组学分析金针菇正常和退化菌株的碳氮代谢途径[J].北方园艺,2025(11):40-47.[67]CHEN B X,WEI T,YE Z W,et al.Efficient CRISPR-Cas9 gene disruption system in edible-medicinal mushroom Cordyceps militaris[J].Frontiers in Microbiology,2018(9):1157.[68]LIN Y,CRADICK T J,BROWN M T,et al.CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences[J].Nucleic Acids Research,2014,42(11):7473-7485.[69]YIN J,LIU M,LIU Y,et al.Optimizing genome editing strategy by primer-extension-mediated sequencing[J].Cell Discovery,2019(5):18.[70]李荣平,李荣春.CRISPR/Cas9基因编辑技术在食用菌中的应用现状[J].生物工程学报,2024,40(4):988-1001.[71]王欣,徐一亿,徐扬,等.作物全基因组选择育种技术研究进展[J].生物技术通报,2024,40(3):1-13.[72]XU Y,ZHANG X,LI H,et al.Smart breeding driven by big data,artificial intelligence,and integrated genomic-enviromic prediction[J].Molecular Plant,2022,15(11):1664-1695.
相似文献/References:
[1]胡艳英,刘 颖. 构建黑龙江省食用菌供应链的探讨[J].北方园艺,2014,38(12):193.
HU Yan-ying,LIU Ying.Discussion on Construction Supply Chain of Edible Fungus of Heilongjiang Province[J].Northern Horticulture,2014,38(17):193.
[2]吴 薇,李文龙,张跃华,等.伊春仙翁山国家森林公园食用菌资源调查[J].北方园艺,2014,38(13):149.
WU Wei,LI Wen-long,ZHANG Yue-hua,et al.Resources Survey of Mushroom in Yichun Seno Mountain National Forest Park[J].Northern Horticulture,2014,38(17):149.
[3]张浩,张焕仕,王猛,等.我国食用菌栽培技术研究进展[J].北方园艺,2014,38(05):175.
ZHANG Hao,ZHANG Huan-shi,WANG Meng,et al.Research Advance on Cultivation Techniques of Mushrooms in China[J].Northern Horticulture,2014,38(17):175.
[4]孙元.弹尾虫对食用菌的危害及防治[J].北方园艺,2012,36(17):143.
SUN Yuan.Damage of Collembola on Edible Fungi and its Control Strategy[J].Northern Horticulture,2012,36(17):143.
[5]暴增海,石桐磊.食用菌的抑菌作用[J].北方园艺,2012,36(18):174.
BAO Zeng-hai,SHI Tong-lei.Anti-Bacterial Functions of Edible Fungus[J].Northern Horticulture,2012,36(17):174.
[6]丁玉萍,韩诚武,赵永勋,等.几种食用菌产胞外纤维素酶对比及其产酶条件的优化[J].北方园艺,2012,36(23):119.
DING Yu-ping,HAN Cheng-wu,ZHAO Yong-xun,et al.Comparison of Extracellular Cellulase of Several Edible Fungus and Optimization of Fermentation Condition[J].Northern Horticulture,2012,36(17):119.
[7]周帅,赵敬领,王家才,等.商丘市食用菌产业现状及发展对策[J].北方园艺,2012,36(08):173.
ZHOU Shuai,ZHAO Jing-ling,WANG Jia-cai,et al.Present and Development Strategy of Edible Fungi Industry in Shangqiu[J].Northern Horticulture,2012,36(17):173.
[8]刘秉儒,宋乃平,苏建宇,等.以柠条为主要基质栽培食用菌的配方筛选研究[J].北方园艺,2012,36(24):168.
LIU Bing-ru,SONG Nai-ping,SU Jian-yu,et al.Selecting Formula for Edible Fungi Using Caragana intermedia Powder as Main Cultivation Material[J].Northern Horticulture,2012,36(17):168.
[9]王相刚,张腾霄,王斌.浅析东北三省食用菌产业的发展及格局变迁[J].北方园艺,2012,36(09):195.
WANG Xiang-gang,ZHANG Teng-xiao,WANG Bin.Analysis of the Development of Edible Fungi Industry and Its Situation’s Vicissitude of Three Provinces in Northeast China[J].Northern Horticulture,2012,36(17):195.
[10]潘春磊,盛春鸽,黄文,等.食用菌保健功能及产品开发技术研究进展[J].北方园艺,2014,38(15):197.
PAN Chun-lei,SHENG Chun-ge,HUANG Wen,et al.Advances on Healthful Function and Processing Technology of Edible Mushroom[J].Northern Horticulture,2014,38(17):197.
备注/Memo
第一作者简介:曾雨康(1999-),男,硕士,研究实习员,现主要从事分子遗传育种等研究工作。E-mail:15727549889@163.com.责任作者:李荣春(1959-),男,博士,教授,现主要从事食用菌遗传育种等研究工作。E-mail:18087762026@163.com.基金项目:云南省(珍稀食用菌)企业技术中心专项资金资助项目(JSJ2023016KJ)。收稿日期:2025-01-24