ZOU Jiayi,ZHANG Long.Identification and Expression Analysis of Dendrobium catenatum ZF-HD Gene Family[J].Northern Horticulture,2025,(12):19-28.[doi:10.11937/bfyy.20250101]
铁皮石斛ZF-HD基因家族鉴定与表达分析
- Title:
- Identification and Expression Analysis of Dendrobium catenatum ZF-HD Gene Family
- 文章编号:
- 1001-0009(2025)12-0019-10
- Keywords:
- Dendrobium catenatum; ZF-HD gene; transcriptome; bioinformatics
- 分类号:
- R 931.2
- 文献标志码:
- A
- 摘要:
- 以铁皮石斛为试材，采用生物信息学和转录组学研究方法，研究了铁皮石斛锌指同源结构域(zinc finger-homeodomain，ZF-HD)基因家族成员编码蛋白的理化性质、系统进化、保守结构域、蛋白基序，基因启动子顺式作用元件和基因表达模式，以期为进一步进行铁皮石斛ZF-HD基因的分子生物学研究提供参考依据。结果表明：铁皮石斛19个ZF-HD基因编码的蛋白质氨基酸残基数范围在85~312，蛋白分子量在9.68~34.04 kDa，理论等电点范围在6.59~9.79，亚细胞定位预测结果显示16个蛋白定位于细胞核，2个定位于线粒体，1个定位于叶绿体；铁皮石斛ZF-HD基因家族包含ZHD-Ⅰ、ZHD-Ⅱ、ZHD-Ⅲ、ZHD-Ⅴ和MIF等五小类；铁皮石斛ZF-HD基因顺式作用元件包含植物激素、非生物胁迫响应以及生长发育控制；DcZHD05基因上游启动子含有MeJA响应的顺式作用元件，在MeJA处理后其表达水平上调；DcZHD15基因上游启动子含有低温响应的顺式作用元件，在冷害处理后其表达水平上调。
- Abstract:
- Taking Dendrobium catenatum as the experimental material，bioinformatics and transcriptomics approaches were employed to investigate the physicochemical properties，evolutionary relationships，conserved domains，protein motifs，cis-acting elements in gene promoters，and gene expression patterns of the zinc finger-homeodomain (ZF-HD) gene family members,in order to provide reference for further molecular biological research on the ZF-HD genes in Dendrobium catenatum.The results showed that the 19 ZF-HD genes in Dendrobium catenatum encode proteins with amino acid residue counts ranging from 85 to 312，molecular weights ranging from 9.68 kDa to 34.04 kDa，and theoretical isoelectric points between 6.59 and 9.79.Subcellular localization predictions revealed that 16 of the proteins were localized to the nucleus，2 to the mitochondria，and 1 to the chloroplast.The Dendrobium catenatum ZF-HD gene family consists of five subfamilies：ZHD-Ⅰ，ZHD-Ⅱ，ZHD-Ⅲ，ZHD-Ⅴ，and MIF.Cis-acting elements of the ZF-HD genes include those responsive to plant hormones，abiotic stress，and growth and development regulation.The promoter of the DcZHD05 gene contains MeJA-responsive cis-acting elements，and its expression level was upregulated following MeJA treatment.The promoter of the DcZHD15 gene contains low-temperature-responsive cis-acting elements，and its expression level was upregulated following cold stress treatment.
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备注/Memo
第一作者简介：邹佳奕（2000-），女，硕士研究生，研究方向为铁皮石斛栽培生理和分子生物学。E-mail：1661746883@qq.com.责任作者：张龙（1986-），男，博士，副教授，现主要从事铁皮石斛栽培生理和分子生物学等研究工作。E-mail：ezhanglong@zju.edu.cn.基金项目：国家自然科学基金资助项目(31801306);丽水市重点研发计划资助项目(2023zdyf09)。收稿日期：2025-01-09