CAO Yu,LI Xintong,LIU Peng,et al.Morphological and Salt Tolerance Analysis of Hybrid Offspring of Chrysanthemum morifolium Ramat. ‘Yannong Shuwan’ and ‘Yannong Zhuyan’[J].Northern Horticulture,2025,(15):98-107.[doi:10.11937/bfyy.20244860]
地被菊‘延农舒绾’ב延农朱颜’杂交后代形态学及耐盐性分析
- Title:
- Morphological and Salt Tolerance Analysis of Hybrid Offspring of Chrysanthemum morifolium Ramat. ‘Yannong Shuwan’ and ‘Yannong Zhuyan’
- 文章编号:
- 1001-0009(2025)15-0098-10
- Keywords:
- ground cover chrysanthemum; hybridization; salt tolerance; morphological characteristics; gene expression
- 分类号:
- S 682.2+1
- 文献标志码:
- A
- 摘要:
- 以地被菊(Chrysanthemum morifolium Ramat.)‘延农舒绾’和‘延农朱颜’为试材，采用人工杂交的方法得到杂交后代，水培盐处理7 d，研究盐胁迫对杂交后代形态和耐盐性的影响，以期为地被菊耐盐品种培育提供参考依据。结果表明：杂交后代植株的叶长、叶宽、冠幅以及花序直径杂种优势呈降低趋势，表现出显性遗传的效应；而株高、管状花数、管状花长、舌状花数、舌状花长均超过双亲。将杂交后代进行盐处理，发现超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性和过氧化氢酶(CAT)活性、脯氨酸(Pro)含量随盐浓度升高呈上升趋势，杂交后代均高于父本‘延农朱颜’并低于母本‘延农舒绾’，且丙二醛(MDA)含量低于‘延农朱颜’。同时，盐处理后对杂交后代及亲本叶片进行DAB和NBT染色，观察受损伤程度，比较后发现其后代叶片损伤程度高于‘延农舒绾’。进一步分析杂交后代及亲本耐盐性相关基因CmSOS1、CmSOS2、CmSOS3、CmNHX1、CmHKT和 CmAKT1表达，盐浓度为200 mmol·L-1时，杂交种和父本的CmSOS1、CmSOS2、CmSOS3、CmNHX1、CmHKT和 CmAKT1表达量均高于母本，而CmAKT1基因表达量均低于母本。该研究获得耐盐性较强的杂交种，丰富了盐碱地区地被菊种类。
- Abstract:
- Taking ground cover chrysanthemum (Chrysanthemum morifolium Ramat.) ‘Yannong Shuwan’ and ‘Yannong Zhuyan’ as experimental materials，the hybrid offspring were obtained by artificial hybridization.The hybrid offspring were treated with hydroponic salt for a week to study the effect of salt stress on the morphology and salt tolerance of hybrid offspring，in order to provide reference for the cultivation of salt tolerant varieties of ground cover chrysanthemum.The results showed that the hybrid offspring plants exhibited a decreasing trend in leaf length，leaf width，crown width，and inflorescence diameter，demonstrating a dominant genetic effect.And the plant height，number of tubular flowers，length of tubular flowers，number of tongue flowers，and length of tongue flowers all exceed those of the parents.After salt treatment of the hybrid offspring，it was found that the activities of superoxide dismutase (SOD)，peroxidase (POD)，catalase (CAT)，and proline (Pro) increased with increasing salt concentration.The hybrid offspring were higher than the male parent ‘Yannong Zhuyan’ and lower than the female parent ‘Yannong Shuwan’，and the content of malondialdehyde (MDA) was lower than ‘Yannong Zhuyan’.At the same time，DAB and NBT staining were performed on the hybrid offspring and parental leaves after salt treatment to observe the degree of damage.After comparison，it was found that the degree of damage to the offspring leaves was higher than that of ‘Yannong Shuwan’.Further analysis of the expression of salt tolerance related genes CmSOS1，CmSOS2，CmSOS3，CmNHX1，CmHKT，and CmAKT1 in hybrid offspring and parents revealed that at a salt concentration of 200 mmol·L-1，the expression levels of CmSOS1，CmSOS2，CmSOS3，CmNHX1，CmHKT，and CmAKT1 in the hybrid and paternal parents were higher than those in the maternal parent，while the expression levels of the CmAKT1 gene were lower than those in the maternal parent.This study obtained hybrid varieties with strong salt tolerance，enriching the variety of ground cover chrysanthemums in saline alkali areas.
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备注/Memo
第一作者简介：曹煜（1999-），女，硕士研究生，研究方向为园艺学。E-mail：2775580474@qq.com.责任作者：高日（1984-），男，博士，副教授，现主要从事菊花分子育种等研究工作。E-mail：gaori@ybu.edu.cn.基金项目：国家自然科学基金资助项目(32060696)；延边大学博士科研启动基金资助项目(2018)；吉林省教育厅科学技术研究资助项目(JJKH20220541KJ）。收稿日期：2024-12-09