LAI Shufang.Effects of Soybean Molasses on the Quality of Hypsizygus marmoreus Liquid Spawn[J].Northern Horticulture,2024,(3):119-126.[doi:10.11937/bfyy.20232354]
大豆糖蜜对斑玉蕈液体菌种质量的影响
- Title:
- Effects of Soybean Molasses on the Quality of Hypsizygus marmoreus Liquid Spawn
- 文章编号:
- 1001-0009(2024)03-0119-08
- Keywords:
- Hypsizygus marmoreus; soybean molasses; liquid spawn quality; physiological and biochemical activity
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以廉价原料大豆糖蜜（soybean molasses，Sm）为试材，采用生理生化指标（生物量、菌球形态、糖代谢速度、胞外酶活力、走菌速度和出菇产量）测定比较法，研究了Sm不同添加量对斑玉蕈液体菌种质量的影响，以期为高值化利用廉价原料Sm制备斑玉蕈液体菌种提供参考依据。结果表明:Sm的适宜添加量为1%，液体培养基中添加1% Sm可以促进斑玉蕈的糖代谢速度，菌丝生长加快，培养至6 d即可达到稳定期，获得的液体菌种生物量更高，且菌球大小均一、无毛刺，菌种表观质量明显提高；液体菌种生理生化活性测定结果表明，添加Sm可以促进胞外蛋白质的分泌，培养基中木聚糖酶、纤维素酶和漆酶活力提高，尤其是漆酶，酶活力是对照的30.4倍，从而加快对Sm中酚酸类物质的降解，培养基pH快速升高，获得的斑玉蕈Sm液体菌种菌球回接后菌丝萌发快、菌丝平均生长速度比对照提高了5.3%；出菇试验表明，斑玉蕈Sm液体菌种相比对照菌丝满袋时间提早4~9 d，去头出菇产量提高6.9%。试验结果表明培养基中添加1% Sm可以缩短斑玉蕈液体菌种制种时间，且有效提高液体菌种质量。
- Abstract:
- The inexpensive raw material soybean molasses (Sm) was used as exogenous additive materials to study the effects of different Sm additions on Hypsizygus marmoreus liquid spawn quality by determination and comparison of physiological and biochemical indexes including biomass，mycelial pellets morphology，glucose consumption rate，extracellular enzyme activity，mycelial growth rate，and fruiting yield,in order to provide reference for the high-value utilization of the inexpensive raw material Sm on H.marmoreus liquid spawn preparation.The results showed that Sm proper addition level was 1% in liquid culture medium.Addition of 1% Sm could improve glucose consumption rate and mycelial growth performance.Stable growth phase of H.marmoreus liquid spawn advanced to 6 days with more biomass obtained comparing to the contrast.The apparent quality of liquid spawn was significantly improved with uniform diameter of mycelium pellets and burr-free on surface.Physiological and biochemical activity of liquid culture medium determination results showed that activity of xylanase，cellulase and laccase increased due to H.marmoreus accelerated secretion extracellular protein with Sm addition.In particular，the activity of laccase was 30.4 times that of the control，in order to degrade phenolic acids in Sm，which lead to the pH of liquid culture medium increased rapidly.Furthermore，mycelial germination was fast and 5.3% increased of average growth rate than the contrast.Finally，fruiting test showed mycelium fulfill the bag advanced 4-9 days and fruiting yield increased 6.9% by using Sm liquid spawn comparing to the contrast.The experiment showed that addition of 1% Sm in the liquid culture medium could shorten the liquid spawn preparation time and effectively improve the quality.
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备注/Memo
作者简介：赖淑芳（1990-），女，硕士，农艺师，现主要从事食用菌技术推广等工作。E-mail:317136858@qq.com.收稿日期：2023-07-12