XIE Hui,YAN Wenjing,Gulimire·KASIMU,et al.Effects of Storage and Transportation Modes on the Viability and Fruiting Characteristics of Apricot Pollen[J].Northern Horticulture,2023,(22):38-44.[doi:10.11937/bfyy.20231832]
贮运模式对杏花粉活力及结实特性的影响
- Title:
- Effects of Storage and Transportation Modes on the Viability and Fruiting Characteristics of Apricot Pollen
- 文章编号:
- 1001-0009(2023)22-0038-07
- Keywords:
- picking period; mode of transportation; storage conditions; pollen vitality; fruit sitting rate; fruit quality
- 分类号:
- S 662.2
- 文献标志码:
- A
- 摘要:
- 以吊干杏花粉为试材,采用琼脂离体培养法,研究了不同蕾期的花粉活力、不同运输方式、不同贮藏温度下的花粉活力及贮藏花粉对坐果率和果实品质的影响,以期为杏花粉采集、运输、贮藏及提升杏坐果率提供参考依据。结果表明:分析不同花蕾采摘时期的花粉活力发现,大蕾期萌发率为70.82%,而中、小蕾期萌发率分别为12.58%、1.66%,且具有极显著性差异(P≤0.01)。不同贮运方式下,冷链运输(8 d)花粉萌发率比常温下高11.10%,冷链的花粉管长度比常温下长370.43 μm,短距离(4 d内)可选常温运输,长距离(4 d以上)选择冷链运输。不同贮藏温度对花粉活力的影响结果表明,随着贮藏时间的延长,花粉萌发率均呈下降趋势;常温下,花粉贮藏90 d时,萌发率为0%;而-40、-20 ℃贮藏360 d时花粉萌发率比较高分别为19.21%、18.13%,差异性较小,均可选作贮藏温度。不同品种的花粉在-20 ℃贮藏360 d,坐果率在10%左右,采摘果实经过品质测定均呈现良好特性。综合各方研究,大蕾期花粉可作为贮藏的花粉。花粉运输时间大于4 d时,建议选择冷链运输。-20、-40 ℃均可作为杏花粉贮藏温度。-20 ℃贮藏360 d的花粉进行果树授粉可保证坐果率,果实品质呈现良好特性。
- Abstract:
- The pollen of Prunus armeniaca L.cv.Diaogan was used as the test material.In vivo agar culture was used to study the pollen viability under different bud stages,different transport modes and different storage temperatures,and effects of pollen storage on fruit set rate and fruit quality,in order to provide reference for apricot pollen collection,transportation,storage and improvement of apricot fruit set rate.The results showed that,the pollen vigor of different bud picking periods showed that the large bud stage germination rate was 70.82%,while the middle and small bud stage germination rate was respectively 12.58% and 1.66%,with highly significant differences (P0.01).Under different storage and transportation modes,the germination rate of pollen in cold chain transportation (8 days) was 11.10% higher than that at room temperature,the length of pollen tubes in the cold chain was 370.43 μm longer than that at room temperature.The short distance (within 4 days) was optional room temperature transportation,and the long distance (more than 4 days) was selected for cold chain transportation.The results of the effect of different storage temperatures on pollen activity showed that with the extension of storage time,the pollen germination rate showed a downward trend at different temperatures.At room temperature,when pollen was stored for 90 days,the germination rate was 0%.However,when stored at -40 ℃ and -20 ℃ for 360 days,the pollen germination rate was relatively high,which were 19.21% and 18.13%,respectively,and the difference was small.Both were selected as storage temperature.The pollen of different varieties was stored for 360 days,and the fruit setting rate could reach more than 10%,and the picked fruits showed good characteristics after quality testing.Based on the research of all parties,the large bud stage could be used as stored pollen.When the pollen transportation time was longer than 4 days,it was recommended to choose cold chain transportation.-20 ℃ and -40 ℃ were suitable temperatures for pollen storage.Pollen stored at -20 ℃ for 360 days for pollination can ensure fruit set rate and fruit quality with good characteristics.
参考文献/References:
[1]陈玉玲,宋文清,夏乐晗,等.杏树冻害的发生与防御措施[J].中国果树,2021(6):65-67.[2]李疆,罗淑萍,杨序德,等.花期低温对仁用杏花器官危害程度的影响[J].新疆农业大学学报,2001,24(4):22-24.[3]王彩虹,李嘉瑞.杏花粉的低温与超低温贮藏研究[J].莱阳农学院学报,1996(3):11-15.[4]宋尚伟,闫锋,苗红霞,等.金太阳杏花粉超低温保存方法研究[J].果树学报,2007(5):689-691.[5]朴永浩,曲柏宏,代志国,等.梨花粉贮藏特性与授粉能力的研究[J].北方园艺,2002(5):54-55.[6]王业遴,盛炳成,於镜,等.桃花粉生活力及其贮藏特性的研究[J].中国果树,1987(2):13-17.[7]曲柏宏,朴永浩,金京南.苹果梨授粉用花粉的采集、运输及贮藏技术[J].延边大学农学学报,2003,25(1):5-11.[8]吴月亮,王明辉,刘明国,等.山杏花粉生活力及其贮藏力的研究[J].沈阳农业大学学报,2013,44(4):479-482.[9]贾文庆,郭英姿,赵国栋,等.西藏黄牡丹花粉生活力、寿命及贮存生理机制研究[J].植物生理学报,2021,57(2):501-511.[10]孙慧瑛,安晓芹,廖康,等.不同培养条件对杏花粉萌发和生长的影响[J].新疆农业大学学报,2012,35(3):173-177.[11]李鹏.猕猴桃花粉活力及人工授粉技术研究[D].杨凌:西北农林科技大学,2017.[12]刁永强,耿文娟,廖康,等.新疆野生杏花粉量及花粉活力研究初报[J].新疆农业科学,2012,49(1):40-45.[13]陈唯.梨不同品种授粉对其杂交果实品质及种子特性的影响[D].杭州:浙江农林大学,2017.[14]谢辉,艾尼瓦尔·肉孜,王乔,等.新疆杏产业发展现状分析及前景展望[J].中国果树,2019(2):108-112.[15]周书娟.树上干杏种质资源遗传多样性及抗寒性研究[D].杨凌:西北农林科技大学,2011.[16]权赫根,于楷力,洪国,等.曼陀罗雄配子体发育及花粉活力测定研究[J].延边大学农学学报,2021,43(2):25-30.[17]FENG X Q,DICKINSON H G.Packaging the male germline in plants[J].Trends Genet,2007(23):503-510.[18]HETHERINGTON S E,SMITH M K,VITHANAGE V,et al.Effect of temperature on pollen germination,pollen tube growth and seed development in mango(Mangifera indica L.)[J].Journal of Horticultural Science & Biotechnology,2000,75(2):214-222.[19]苗卫东,王萌,井朋伟,等.不同葡萄品种花粉生活力测定与贮藏条件[J].北方园艺,2021(24):23-31.[20]王飞,陈登文,李嘉瑞,等.杏花及幼果人工模拟冻害及生理研究[J].西北农业学报,1999(1):95-97.[21]DICHINSON H G,GRANT-DOWNTON R.Bridging the generation gap:Flowering plant gametophytes and animal germlines reveal unexpected similarities[J].Biological Reviews,2009,84(2):589-615.[22]李硕,胡亚洲,冯建荣,等.不同贮藏方式下新疆5个杏品种花粉活力比较[J].新疆农业科学,2014,51(1):29-34.[23]安晓芹,王玲丽,逯东阳,等.杏花粉活力测定培养基筛选及耐贮性研究[J].新疆农业科学,2011,48(11):1979-1982.[24]孙超,台秀国,杨振亚,等.不同贮藏温度对4种杨树花粉生活力的影响[J].山东科学,2015,28(1):109-113.[25]RAJASEKHARAN P E,GANESHAN S.Current perspectives on pollen cryopreservation in horticultural species[J].Acta Hortic,2019,1234:47-56.[26]王钦丽,卢龙斗,吴小琴,等.花粉的保存及其生活力测定[J].植物学通报,2002(3):365-373.[27]徐刚标.植物种质资源离体保存研究进展[J].中南林学院学报,2000(4):81-87.[28]吴俊,谷超,张绍铃,等.中国杏自交不亲和花粉特异SFB基因的鉴定与序列分析[J].园艺学报,2010,37(8):1329-1338.[29]安晓芹,廖康,刘娟,等.新疆杏品种授粉特性研究[J].新疆农业大学学报,2014,37(4):281-286.[30]廖明康,郭丽霞,张平,等.新疆杏部分生物学性状的鉴定[J].新疆农业科学,1994(2):85-87.[31]蔡倩,孙占祥,郑家明,等.花期低温对仁用杏花粉活力及坐果率的影响[J].湖北农业科学,2016,55(9):2272-2274.[32]刘黎明,刘萍,梁凤丽,等.新疆野杏花粉活力和柱头可授性研究[J].华南农业大学学报,2010,31(4):86-89.
备注/Memo
第一作者简介:谢辉(1984-),男,博士,研究员,现主要从事新疆特色果树栽培与加工等研究工作。E-mail:xhxjnky@163.com.责任作者:张大海(1968-),男,硕士,研究员,现主要从事果树栽培、果树资源、果树育种及农业产业化等研究工作。E-mail:ziyuanpu@sina.com.基金项目:中央引导地方-特色果树种质创新与育种能力提升资助项目;新疆杏产业技术体系专项资金资助项目(XJCYTX-01,XJCYTX-03);天山英才青年科技拔尖人才资助项目(2022TSYCCX0068)。收稿日期:2023-05-27