ZHANG Yanzhao,LUO Liheng,LI Xilie,et al.Response of Cucumber Plant Secondary MetabolismSubstance to Low-light Stress[J].Northern Horticulture,2023,(12):1-7.[doi:10.11937/bfyy.20223959]
黄瓜植株次生代谢物质对弱光胁迫的响应
- Title:
- Response of Cucumber Plant Secondary MetabolismSubstance to Low-light Stress
- Keywords:
- cucumber; low-light stress; secondary substance metabolism; seedlings; responsing charactertics
- 文献标志码:
- A
- 摘要:
- 以耐弱光黄瓜纯合品系M67和不耐弱光黄瓜纯合品系M14为试材，经光强为80 μmol·m-2·s-1的弱光处理后，测定不同时期幼苗叶片中次生物质含量及PAL1基因的表达量,研究了弱光处理对各时期黄瓜幼苗植株次生物质代谢的影响，以期为黄瓜耐弱光机理研究及新品种选育提供参考依据。结果表明：弱光处理后，除M67的一叶一心期幼苗叶片中总酚含量无显著降低外，其它时期2个品系的幼苗叶片中总酚含量均显著低于对照，M14在子叶初展期下降幅度更大；木质素方面，M67各时期幼苗叶片中木质素含量较对照呈不同程度的升高，以一叶一心期升高幅度最大；类黄酮方面，与对照相比，M67各时期幼苗叶片中类黄酮含量无显著降低，M14各时期均显著降低，以两叶一心期幼苗叶片中类黄酮含量下降最为显著；与对照相比，除M67两叶一心期幼苗叶片中苯丙氨酸解氨酶（PAL）活性无显著降低外，2个品系各时期PAL活性均显著降低，此外，各时期M67叶片中CsPAL1基因的表达量没有显著变化，而M14叶片中PAL活性和CsPAL1基因显著降低。受弱光胁迫后耐弱光品系植株能通过保持更稳定的次生代谢物质水平及其基因表达水平以提高其对弱光胁迫的抵抗能力。
- Abstract:
- The low-light tolerant homozygous cucumber line M67 and the low-light sensitive homozygous line M14 were used as test materials，the secondary biomass and the expression of PAL1 gene in leaves of seedlings at different stages were determined after low-light treatment with 80 μmol·m-2·s-1，the characteristics of cucumber plants responding to low light through changes in secondary substance metabolism at different stages were studied,in order to provide a reference for the mechanism studies of cucumber tolerance to low light and the breeding of varieties.The results showed that after low light treatment，except for the total phenol content in the leaf of seedlings of M67 at one-leaf and one-heart stage，the total phenol content in the leaves of both lines in other periods were significantly lower than that of the control，and M14 decreased more significantly at the cotyledon extension stage.The lignin content in the leaves of seedlings in M67 at various stages showed different degrees of increase compared with the control，there was the highest increase at one leaf stage.In terms of flavonoids，there was no significant decrease in the low-light tolerant line M67 compared with the control，and the low-light sensitive line M14 showed a significant decrease in three periods，and the flavonoid content in the leaves of the two-leaf and one-heart stage seedlings was the most significant，compared with the control.The PAL activity in the leaves of seedlings in both lines were significantly reduced，excepting for the significant decrease in PAL activity in the leaves of M67 at two-leaf and one-heart stage seedlings，and there was no change for the expression of CsPAL1 gene in the leaves of the M67 plant，while the expression of CsPAL1 gene in the leaves of the M14 line was significantly reduced.This study showed that the low-light tolerant line plants could improve their resistance to low-light stress by maintaining a more stable level of secondary metabolic substances.
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备注/Memo
第一作者简介：张艳昭（1995-），男，硕士研究生，研究方向为黄瓜耐弱光遗传育种。E-mail：zhangabo151014@163.com.责任作者：李丹丹（1981-），女，博士，副教授，硕士生导师，现主要从事黄瓜抗逆性遗传育种等研究工作。E-mail：lidandan342@126.com.基金项目：国家青年自然科学基金资助项目（32002043）；黑龙江八一农垦大学三横三纵支持计划资助项目（TDJH202004）。收稿日期：2022-09-27