LI Meng,LA Guixiao,ZHANG Bao,et al.Response Characteristics of Rehmannia glutinosa to Drought Stress Under Different Virus-free Treatments[J].Northern Horticulture,2023,(07):111-118.[doi:10.11937/bfyy.20222933]
不同脱毒处理怀地黄对干旱胁迫的响应特性
- Title:
- Response Characteristics of Rehmannia glutinosa to Drought Stress Under Different Virus-free Treatments
- Keywords:
- Rehmannia glutinosa; virus-free; PEG 6000; growth; physiological characteristics
- 文献标志码:
- A
- 摘要:
- 以怀地黄的病毒苗、茎尖脱毒苗、“电”疗法脱毒苗为试材，采用不同浓度PEG 6000（0%、5%、10%、15%）模拟干旱胁迫的方法，研究了干旱胁迫对3种材料形态特征、叶绿素、叶片相对含水量、渗透调节物质及抗氧化酶活性的影响，以期为脱毒地黄在大田栽培上的应用推广提供参考依据。结果表明：PEG 6000胁迫下，3种材料生长明显受到抑制，生根天数延长，株高、生根数、根长、鲜质量均降低，但以“电”疗法获取的脱毒苗生长情况最好；随干旱胁迫程度增加，“电”疗法获取的脱毒苗叶绿素含量先下降后上升，茎尖培养的脱毒苗略微下降，而病毒苗则表现为下降、上升再下降的趋势；叶片相对含水量逐渐降低；而可溶性糖、可溶性蛋白质含量、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、过氧化物酶（POD）活性随胁迫程度加深则呈现出持续上升的趋势，以“电”疗法脱毒苗含量最高。综上可知，“电”疗法脱毒苗抗旱能力较强，可为地黄抗旱机制的研究及田间栽培提供示范参考作用。
- Abstract:
- Taking virus seedlings,shoot tip virus-free seedlings and ‘electric’ therapy virus-free seedlings as experimental materials,the effects of drought stress on morphological characteristics,chlorophyll,relative water content of leaves,osmoregulatory substances and antioxidant enzyme activities of the three materials were studied by simulating drought stress with different concentrations of PEG 6000 (0%,5%,10% and 15%),in order to provide reference for the application and promotion of detoxified groundnut in field cultivation.The results showed that the growth of the three materials was significantly inhibited under PEG 6000 stress.With the extension of rooting days,plant height,rooting number,root length and fresh weight decreased,but the growth of virus-free seedlings obtained by ‘electric’ therapy was the best.With the increase of drought stress,the chlorophyll content of virus-free seedlings obtained by ‘electric’ therapy first decreased and then increased.The virus-free seedlings in shoot tip culture decreased slightly,while the virus-free seedlings showed a downward,upward and then downward trend.The relative water content of leaves decreased gradually.The contents of soluble sugar,soluble protein,and activities of CAT,SOD and POD showed a continuous upward trend with the deepening of stress,and the content of virus-free seedlings treated with ‘electric’ therapy was the highest.Therefore,the ‘electric’ treatment of virus-free seedlings had strong drought resistance,which could provide a demonstration reference for the study of drought resistance mechanism and field cultivation of Rehmannia glutinosa.
参考文献/References:
[1]张晓丽,李萍,周彩云,等.怀地黄脱毒种苗大田生长性状及产量品质[J].植物学报,2017,52(4)：474-479.[2]苏秀红,腊贵晓,刘春朝,等.怀地黄脱毒技术的优选[J].植物生理学报,2022,58(3)：587-595.[3]ABID U,MOHAMMAD N,HAZRAT A,et al.Drought tolerance improvement in plants：An endophytic bacterial approach[J].Applied Microbiology and Biotechnology,2019,103:7385-7397.[4]项洪涛,李琬,何宁,等.外源脱落酸（ABA）调节植物抗旱机制的研究进展[J].东北农业科学,2022,47(5):37-41.[5]田梦平,谢小龙,杜权,等.16份怀地黄种质资源抗旱性评价[J].江苏农业科学,2021,49(21)：153-159.[6]左静静,闫贵云,霍利光,等.脱毒地黄试管苗的微扦插快繁技术研究[J].中药材,2020,43(1)：20-23.[7]陈明霞,赵喜亭,李明军,等.多效唑对脱毒怀地黄光合生理和抗性生理的影响[J].北方园艺,2014(22)：158-161.[8]丁明珠,邓竹根,胡克玲,等.蜜薯的脱毒培养及脱毒苗的生理变化[J].安徽农业大学学报,2021,48(4):560-568.[9]郑小燕,郑柯强,陈巍.脱毒马铃薯的增产潜力及抗逆性试验初探[J].福建农业科技,2010(5)：36-38.[10]石欣隆,杨月琴,薛娴,等.壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响[J].南京林业大学学报(自然科学版),2021,45(2):120-126.[11]李合生．现代植物生理学：植物生理生化实验原理与技术[M].3版.北京：高等教育出版社,2010．[12]高昆,石义妃.干旱胁迫对粉葛幼苗生长及生理特性的影响[J].江苏农业科学,2021,49(8)：153-157.[13]张雕,刘敏婕,刘卫东,等.干旱胁迫对‘菊花桃’幼苗生长及生理特性的影响[J].经济林研究,2021,39(1):211-219.[14]李经纬.马铃薯茎尖与病毒超低温保存技术的研究及超低温疗法脱毒试管苗的耐盐性评价[D].杨凌:西北农林科技大学,2019.[15]杨喜珍,杨利,覃亚,等.PEG-8000模拟干旱胁迫对马铃薯组培苗叶绿素和类胡萝卜素含量的影响[J].中国马铃薯,2019,33(4):193-202.[16]邓珍,徐建飞,段绍光,等.PEG-8000模拟干旱胁迫对 11个马铃薯品种的组培苗生长指标的影响[J].华北农学报,2014(5)：99-106.[17]文爱华,刘济明,高攀,等.自然干旱胁迫对米槁幼苗叶片显微 结构及叶绿素含量的影响[J].北方园艺,2015(14)：62-66.[18]戴海根,董文科,柴澍杰,等.模拟干旱胁迫下鹰嘴紫云英幼苗生长及生理特性[J].中国草地学报,2021,43(10):63-75.[19]陈彦云,李紫辰,曹君迈,等.马铃薯脱毒苗对NaCl胁迫的响应及耐盐性评价[J].西南农业学报,2018,31(10):2052-2059.
相似文献/References:
[1]张丽,石磊,甘晓燕,等.葡萄脱毒培养与病毒检测技术研究进展[J].北方园艺,2012,36(04):174.
ZHANG Li .,SHI Lei .,GAN Xiao-yan.,et al.Advances on Techniques for Virus-free Culture and Virus Detection of Grape[J].Northern Horticulture,2012,36(07):174.
[2]郭超,吴然,邵建柱,等.四个苹果砧木和品种苹果潜隐性病毒的变温热处理脱毒效果分析[J].北方园艺,2014,38(17):130.
GUO Chao,WU Ran,SHAO Jian-zhu,et al.Study on Elimination and Recrudescence of Apple Latent Virus by Alternating Heat Therapy in vitro[J].Northern Horticulture,2014,38(07):130.
[3]朱畇昊,杜真辉,董诚明,等.地黄块根内生真菌的多样性研究[J].北方园艺,2016,40(03):145.[doi:10.11937/bfyy.201603041]
ZHU Yunhao,DU Zhenhui,DONG Chengming,et al.Diversity of Endophytic Fungi From Tubers of Rehmannia glutinosa Libosch[J].Northern Horticulture,2016,40(07):145.[doi:10.11937/bfyy.201603041]
[4]薛 寒 青,王 舰,周 淑 兰,等.大蒜根尖培养诱导不定芽研究[J].北方园艺,2011,35(09):0.[doi:10.11937/bfyy.201109045]
XUE Han -qing,WANG Jian,et al.Induce Adventitious Bud Garlic Root[J].Northern Horticulture,2011,35(07):0.[doi:10.11937/bfyy.201109045]
[5]朴 仁 哲,赵 洪 颜,金 玉 姬,等.百草枯胁迫对地黄叶片膜脂过氧化相关生理指标的影响[J].北方园艺,2008,32(10):0.[doi:10.11937/bfyy.2008010014]
PIAO Ren -zhe,ZHAO Hong -yan,JIN Yu -ji,et al.Effect of Paraquat Stress on Physiological Indexes Related with Membrane Lipid Peroxidation of Rehmanniae Lesves[J].Northern Horticulture,2008,32(07):0.[doi:10.11937/bfyy.2008010014]
[6]于 德 才,李 学 湛,吕 典 秋,等.大 蒜 茎 尖 脱 毒 及 快 繁 研 究[J].北方园艺,2005,29(06):0.[doi:10.11937/bfyy.200506028]
[J].Northern Horticulture,2005,29(07):0.[doi:10.11937/bfyy.200506028]
[7]杜真辉,董诚明,朱畇昊,等.不同灌溉制度对地黄产量及品质的影响[J].北方园艺,2016,40(22):170.[doi:10.11937/bfyy.201622042]
DU Zhenhui,DONG Chengming,ZHU Yunhao,et al.Effects of Different Irrigation System on Yield and Quality of Rehmannia glutinosa[J].Northern Horticulture,2016,40(07):170.[doi:10.11937/bfyy.201622042]
[8]李学慧,张亚菲,刘述忠,等.不同品种和施肥方案对地黄农艺性状及产量的影响[J].北方园艺,2018,42(06):129.[doi:10.11937/bfyy.20172960]
LI Xuehui,ZHANG Yafei,LIU Shuzhong,et al.Effect of Different Varieties and Fertilization Schedules on Agronomic Traits and Yield of Rehmannia glutinosa Libosch[J].Northern Horticulture,2018,42(07):129.[doi:10.11937/bfyy.20172960]
[9]王玉英,王琴,李枝林,等.虎雪兰玻璃化超低温法脱毒技术的研究[J].北方园艺,2020,44(05):61.[doi:10.11937/bfyy.20191600]
WANG Yuying,WANG Qin,LI Zhilin,et al.Study on Cymbidium tracyanum var. huanghua×Cymbidium mastersii on Virus Elimination Using Vitrification-cryopreservation Technology[J].Northern Horticulture,2020,44(07):61.[doi:10.11937/bfyy.20191600]
[10]吴廷娟,田梦平,董诚明,等.不同地黄种质资源光合日动态特性的比较[J].北方园艺,2020,44(05):115.[doi:10.11937/bfyy.20191985]
WU Tingjuan,TIAN Mengping,DONG Chengming,et al.Comparative Study on Photosynthetic Characteristics of Different Rehmannia glutinosa Germplasm Resources[J].Northern Horticulture,2020,44(07):115.[doi:10.11937/bfyy.20191985]
备注/Memo
第一作者简介：李萌(1995-)，女，硕士研究生，研究方向为中药资源学。E-mail:1096483959@qq.com.责任作者：苏秀红（1975-），女，博士，教授，现主要从事中药资源学等研究工作。E-mail:suxiuhong80@163.com.基金项目：河南省重点研发与推广专项资助项目（222102310625，222102310455）；国家现代农业产业技术体系资助项目（CARS-21）;河南省中药材产业技术体系资助项目（HARS-22-11-G2）。收稿日期：2022-07-17