CAI Fuge,BAO Ju,ZHANG Xiuwei,et al.Study on Isolation，Identification and Nutritional Utilization Pattern Characteristics of a Wild Lentinus squarrosulus Strain[J].Northern Horticulture,2022,(24):98-106.[doi:10.11937/bfyy.20221885]
野生翘鳞香菇分离鉴定及其菌丝营养利用规律研究
- Title:
- Study on Isolation，Identification and Nutritional Utilization Pattern Characteristics of a Wild Lentinus squarrosulus Strain
- 文献标志码:
- A
- 摘要:
- 以采自贵州省安顺市镇宁自治县的野生翘鳞香菇（QL-1）为试材，采用子实体形态学和菌丝ITS序列分析法进行鉴定，研究了不同温度、pH、碳源、氮源、维生素、无机盐和栽培基质对菌丝生长的影响，以期优化野生翘鳞香菇培养基和栽培基质，为进一步开发应用提供参考依据。结果表明：QL-1菌株为翘鳞香菇（Lentinus squrrosulus），培养的最佳温度为30 ℃，最适pH为5~6；最佳碳氮源分别为果糖和酵母提取物，菌丝生长速度最快，为1.07 cm·d-1和0.81 cm·d-1；添加麦芽糖和硝酸铵，菌丝的浓密度最佳；培养基中添加适量的维生素B1、维生素B6、硫酸锰和氯化钙能有效促进菌丝生长；在栽培基质中添加20%的玉米芯有助于菌丝体生长。
- Abstract:
- A wild Lentinus squarrosulus strain （QL-1）,which collected from Zhenning county，Anshun city，Guizhou Province was used as test material.The fruiting body morphology and mycelial ITS sequence analysis were used.The effects of temperature，pH，carbon source，nitrogen source，vitamin，inorganic salt and culture substrate on mycelial growth were studied，in order to optimize the culture medium and culture substrate,and provide reference for further development and application.The results showed that the QL-1 strain was Lentinus squarrosulus,the optimum culture temperature was 30 ℃;and the optimum pH was 5-6;the best carbon and nitrogen sources were fructose and yeast extract，the fastest growth rate of mycelia was 1.07 cm·d-1 and 0.81 cm·d-1,respectively;adding maltose and ammonium nitrate in the medium，the mycelia grew thicker;adding proper amount of vitamin B1，vitamin B6，manganese sulfate and calcium chloride in the medium could effectively promote the growth of mycelia;adding 20% corncob to culture substrate was beneficial for mycelium growth.
参考文献/References:
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备注/Memo
第一作者简介：蔡甫格（1986-），男，硕士，高级农艺师，现主要从事野生食用菌驯化栽培与育种等研究工作。E-mail：123364942@qq.com.责任作者：曹家洪（1965-），男，本科，正高级农艺师，现主要从事作物遗传与育种等研究工作。E-mail：jqcjh1965@163.com.基金项目：安顺市科技计划资助项目（安市科农[2020]12号,安市科农重[2020]02号）；贵州省科技计划资助项目（黔科合支撑[2019]2451-4号,黔科合平台人才[2019]5105号,黔科合重大专项[2019]3008号,黔科合支撑[2019]2451-6号）。收稿日期：2022-05-07