TIAN Haoyuan,XU Yanjun,WU Chenyin,et al.Effects of Carbon and Nitrogen Sources on Liquid Culture and Extracellular Enzyme Activities of Pleurotus cornucopiae[J].Northern Horticulture,2022,(19):114-121.[doi:10.11937/bfyy.20221043]
碳氮源对姬菇液体菌种培养及其胞外酶活性影响的探究
- Title:
- Effects of Carbon and Nitrogen Sources on Liquid Culture and Extracellular Enzyme Activities of Pleurotus cornucopiae
- 文献标志码:
- A
- 摘要:
- 以“姬菇1号”为试材，采用不同碳源和氮源制作液体菌种培养基，研究不同碳氮源配方对姬菇菌丝生物量、直径和密度的影响，并作各碳氮源配方对姬菇菌丝培养液胞外酶的诱导研究,以期为进一步优化姬菇液体菌种培养提供参考依据。结果表明：“姬菇1号”液体菌种在以玉米粉为碳源的培养基中表现最好，在以豆粕为氮源的培养基中表现最好。通过双因素饱和-D最优设计得出最优配方DY为玉米粉2.48 g，豆粕1 g，磷酸二氢钾0.3 g，硫酸镁0.2 g，维生素B1 2 mg，菌丝生物量达到1.70 g；碳源中的玉米粉对液体菌种纤维素酶、多酚氧化酶及漆酶的诱导效果较好，以可溶性淀粉为碳源时其过氧化物酶活性最高；而在氮源中集中表现在豆粕培养基，其对纤维素酶、多酚氧化酶及漆酶的诱导作用均是最高，与其它处理具有极显著性差异，在以蛋白胨为氮源时其过氧化物酶活性最高，诱导作用最强。
- Abstract:
- Taking‘Pleurotus cornucopiae 1＃’ as the experimental material,different carbon and nitrogen sources were used to make liquid culture medium to study the effects of different carbon and nitrogen source formulations on the biomass,diameter and density of mycelia of Pleurotus cornucopiae，and the carbon and nitrogen source formula for Pleurotus cornucopiae hypha culture of extracellular enzyme induction study,in order to provide reference for further optimized Pleurotus cornucopiae liquid bacteria culture.The results showed that the liquid strain of ‘Pleurotus cornucopiae 1＃’ had the best performance in the medium with corn flour as carbon source and soybean meal as nitrogen source.The optimal formula DY was obtained by the two-factor saturate-D optimal design,corn flour 2.48 g,soybean meal 1 g,potassium dihydrogen phosphate 0.3 g,magnesium sulfate 0.2 g,vitamin B1 2 mg,the mycelial biomass reached 1.70 g.The corn flour induced cellulase,polyphenol oxidase and laccase of liquid strains better in carbon source,and the peroxidase activity was highest when soluble starch was used as carbon source.Among the nitrogen sources,soybean meal medium had the highest inducing effect on cellulase,polyphenol oxidase and laccase,which was significantly different from other treatments.When peptone was used as nitrogen source,the peroxidase activity was the highest and the inducing effect was the strongest.
参考文献/References:
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备注/Memo
〖KH-1D〗〖FQ(9*3。78mm,ZX-WZ〗〖CD78mm〗第一作者简介：田浩原（1997-），男，硕士研究生，研究方向为蔬菜生理生态与高效栽培。E-mail:a1798310906@163.com.责任作者：徐彦军（1972-），男，硕士，教授，现主要从事食用菌教学与科研等研究工作。E-mail:gdxyj1996@126.com.基金项目：贵州省科技厅资助项目（黔科合重大专项字[2019]3005，黔科合服企[2018]4002-2）。收稿日期：2022-03-20