ZHAO Hong,XU Fenfen,YU Shuling,et al.Mitigation Effect of 2，4-Epibrassinolide on Cucumber Seedlings Under Cadmium Stress[J].Northern Horticulture,2022,(20):35-41.[doi:10.11937/bfyy.20220655]
2,4-表油菜素内酯对镉胁迫下黄瓜幼苗的缓解效应
- Title:
- Mitigation Effect of 2，4-Epibrassinolide on Cucumber Seedlings Under Cadmium Stress
- Keywords:
- cucumber; 2; 4-Epibrassinolide; cadmium stress; mitigation effect
- 文献标志码:
- A
- 摘要:
- 以“津优35号”黄瓜为试材，采用营养液水培的方法，研究了喷施不同浓度(0.05、0.1、0.5、1.0 mg·L-1)2,4-表油菜素内酯(EBR)对镉胁迫下黄瓜幼苗生长、根系活力、抗氧化酶活性、渗透调节物质含量及丙二醛（MDA）含量的影响，以期为黄瓜的耐镉栽培提供参考依据。结果表明：10 mg·L-1镉处理显著抑制了黄瓜幼苗形态生长和生物量的累积，显著降低了超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性及根系活力，显著增加了脯氨酸、可溶性糖、可溶性蛋白质及MDA含量。喷施不同浓度EBR后，均使镉胁迫下黄瓜幼苗抗氧化酶活性、根系活力、渗透调节物质含量显著增大，MDA含量显著下降，幼苗生长各项指标不同程度提高。与单独镉胁迫相比，喷施0.5 mg·L-1 EBR处理使黄瓜幼苗SOD、POD、CAT活性及根系活力分别提高了75.81%、45.08%、59.98%、70.64%，脯氨酸、可溶性糖、可溶性蛋白质含量分别增加了69.83%、90.52%、40.38%，MDA含量降低了38.18%。综上，喷施EBR能够提高黄瓜幼苗的抗氧化能力和渗透调节能力，减轻镉胁迫造成的膜脂过氧化水平，缓解镉胁迫对黄瓜幼苗生长的抑制，其中以EBR浓度为0.5 mg·L-1时效果最好。
- Abstract:
- Cucumber variety ‘Jinyou 35’ were used as experimental material,effects of spraying different concentrations (0.05,0.1,0.5,1.0 mg·L-1) EBR on the growth,root and antioxidant enzyme activities,osmotic substance and MDA contents in cucumber seedlings were studied by hydroponic method of nutrient solution under cadmium stress,in order to provide reference for cadmium tolerance cultivation of cucumber.The results showed that 10 mg·L-1 cadmium treatment significantly inhibited the morphological indicators and biomass accumulation of cucumber seedlings,decreased the activities of SOD,POD,CAT and root,increased the contents of proline,soluble sugar,soluble protein and MDA.Under cadimium stress,the antioxidant enzyme activities,root activity,osmotic substance contents of cucumber seedlings were significantly increased,while the content of MDA was significantly decreased,all growth indicators were improved in different extent after spraying EBR at different concentrations.Compared with cadimium stress alone,spraying 0.5 mg·L-1 EBR increased the activities of SOD,POD,CAT and root of cucumber seedings by 75.81%,45.08%,59.98%,70.64% respectively,increased the contents of proline,soluble sugar,soluble protein by 69.83%,90.52%,40.38% respectively,decreased the MDA content by 38.18%.In conclusion,spraying EBR could reduce the level of membrance lipid peroxidation caused by cadmium stress,mitigate the inhibition of cadmium stress on growth of cucumber seedlings,which could be attributed to the improving of the antioxidant and osmotic adjustment ability.EBR concentration of 0.5 mg·L-1 was the best.
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备注/Memo
第一作者简介：赵红（1966-），女，硕士，高级实验师，现主要从事植物生理学教学与科研等工作。E-mail:1024zhaohong@163.com.基金项目：江西省教育厅科学技术研究资助项目（GJJ170941）。收稿日期：2022-02-24