TIAN Yunfang,CAO Tingting,ZHOU Mingmei,et al.Extraction and Antioxidant Activity of Flavonoids From Different Tissues of Crepidiastrum denticulatum[J].Northern Horticulture,2022,(17):100-104.[doi:10.11937/bfyy.20220539]
黄瓜假还阳参不同部位黄酮提取及抗氧化活性
- Title:
- Extraction and Antioxidant Activity of Flavonoids From Different Tissues of Crepidiastrum denticulatum
- 文献标志码:
- A
- 摘要:
- 以黄瓜假还阳参为试材，采用60%的乙醇超声提取黄瓜假还阳参的根、老茎、嫩茎、叶、花蕾、花、种子中的总黄酮，用分光光度法测定各部位的总黄酮含量以及对DPPH自由基、羟基自由基的清除能力和还原力等抗氧化活性指标，通过测定不同部位的总黄酮含量及比较其抗〖JP+2〗氧化活性，以期为开发利用黄瓜假还阳参药用资源提供参考依据。结果表明：黄瓜假还阳参各部位的总黄酮（以芦丁计）含量由高到低依次为种子3.75 mg?g-1、叶2.14 mg?g-1、花蕾0.93 mg?g-1、花（盛开期）0.69 mg?g-1、嫩茎0.44 mg?g-1、根0.34 mg?g-1、老茎0.17 mg?g-1。各部位提取物的DPPH自由基、羟基自由基清除能力和Fe2+还原力也存在差异，且抗氧化活性与提取物中总黄酮含量存在量效关系，种子中的总黄酮抗氧化活性最强。其开发利用以提取黄酮为目的时，黄瓜假还阳参适宜于种子成熟期采收；因叶片产量远高于种子，其黄酮含量也仅次于种子，也可选择叶片生长旺盛时期采收。
- Abstract:
- Taking Crepidiastrum denticulatum as test merterial，the total flavonoids in the roots，old stems，tender stems，leaves，flower buds，flowers and seeds of Crepidiastrum denticulatum were extracted by ultrasonic extraction with 60% ethanol，the contents of total flavonoids and antioxidant activities such as DPPH，hydroxyl radical scavenging activity and reducing power were determined by spectrophotometer.The contents of total flavonoids in different parts of the plant were determined and their antioxidant activities were compared,in order to provide reference for the development and utilization of the medicinal resources of Crepidiastrum denticulatum.The results showed that the total flavonoid contents of seed extract(3.75 mg?g-1) were higher than those of leaves (2.14 mg?g-1)，buds (0.93 mg?g-1)，blooming flowers (0.69 mg?g-1)，tender stems (0.44 mg?g-1)，roots (0.34 mg?g-1) and old stems (0.17 mg?g-1).The scavenging capacity of DPPH free radical，hydroxyl free radical and Fe2+ reduction capacity of the extracts from different tissues were also different，and there was a dose-effect relationship between the antioxidant activity and the content of total flavonoids in the extracts.The antioxidant activity of total flavonoids in seeds was the strongest.For the purpose of extracting flavonoids，Crepidiastrum denticulatum was suitable for harvesting in the mature stage of seeds.Because the yield of leaves was higher than that of seeds，the content of flavonoids was the second to the seed，so it is also possible to select the period of vigorous leaf growth to harvest.
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备注/Memo
第一作者简介：田云芳（1978-），女，博士，副教授，现主要从事药用植物资源开发等研究工作。E-mail：tianyunfang2011@163.com.基金项目：河南省科技攻关资助项目(212102110184)；河南省高等学校重点科研资助项目(22A210026)；河南省高等学校青年骨干教师培养计划资助项目(2018GGJS162)。收稿日期：2022-02-16