WANG Junqing,ZHANG Yanhui,YI Wanying,et al.Study on Genes Related to Anthocyanin Biosynthesis at the Color Turning Stages in Red Okra Fruit Based on Transcriptome Sequencing[J].Northern Horticulture,2022,(14):9-16.[doi:10.11937/bfyy.20220291]
基于转录组测序红秋葵果实转色期花青素生物合成相关基因研究
- Title:
- Study on Genes Related to Anthocyanin Biosynthesis at the Color Turning Stages in Red Okra Fruit Based on Transcriptome Sequencing
- 文献标志码:
- A
- 摘要:
- 以红秋葵果实发育转色期为研究对象，运用Illumina HiSeq 2500高通量测序技术对其转色期进行转录组测序，研究红秋葵果实转色期花青素合成相关基因，以期从差异表达的基因中鉴定得到花青素生物合成的相关酶基因。结果表明：在红秋葵果实发育转色期转录本中共获得208 145条Unigenes，对不同果实发育转色期差异表达的基因进行KEGG富集分析，共鉴定到28条花青素生物合成相关的Unigenes。根据其在红秋葵果实发育转色期表达情况，推测[STBX]CHS4、CHS6、CHS8、HCT1、HCT2、CHI、UFGT和F3′5′H[STBZ]这8条基因在花青素生物合成中起关键作用。
- Abstract:
- The red okra fruit development and color turning periods (three key development periods) were used as research object.The transcriptome of the color turning stages in red okra fruit were sequenced by Illumina HiSeq 2500 sequencing technology,the related enzyme genes of anthocyanin biosynthesis were identified from differentially expressed genes.In the transcripts of three key development periods,a total of 208 145 Unigenes were assembled,Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of genes differentially expressed in different tissues showed that a total of 28 differentially expressed genes related to anthocyanin synthesis were identified.According to the expression level analysis of genes at the color turning stages in red okra fruit,8 Unigenes of 〖STBX〗CHS4,CHS6,CHS8,HCT1,HCT2,CHI,UFGT and F3′5′H〖STBZ〗 were assummed as the key genes of anthocyanin synthesis.
参考文献/References:
[1]赖正锋，练冬梅，姚运法，等.黄秋葵新品种‘闽秋葵3号’[J].园艺学报，2019，46(6)：1217-1218.[2]练冬梅，赖正锋，姚运法，等.黄秋葵新品种‘闽秋葵4号’[J].园艺学报，2019，46(7)：1423-1424.[3]高尚，高玲，张如莲，等.黄秋葵营养与功能成分研究进展[J].中国热带农业，2015，63(2)：46-49.[4]OLAYIWOLA M O，ARIYO O J.Relative discriminatory ability of GGE Biplot and YSi in the analysis of genotype×environment interaction in okra (Abelmoschus esculentus)[J].International Journal of Plant Breeding and Gentics，2013，12(7)：146-158.[5]ISLAM M，HOSSAIN N，ALAMGIR M，et al.Comparative effects of biogas plant residues poultry manure and inorganic fertilizer on growth and yield of ladies finger[J].IOSR Journal of Agriculture and Veterinary Science，2014，7(1)：2319-2372.[6]徐康.采收期对黄秋葵果实品质及风味物质的影响[J].食品与发酵工业，2015，41(4)：207-211.[7]魏聪.黄秋葵花种子及果皮营养成分与活性成分研究[D].乌鲁木齐:新疆农业大学，2015.[8]李军民.黄秋葵的药用(营养)价值及露地栽培技术[J].现代农业科技，2015(2)：118-119.[9]李莹，刘一桐，高铭彤，等.黄秋葵化学成分及药理作用研究进展[J].时珍国医国药，2017，28(3)：683-685.[10]王君耀，周俊，汤谷平.黄秋葵抗疲劳作用的研究[J].现代应用药学杂志，2003，20(4)：316-317.[11]YUAN C Y，ZHANG C，WANG P，et al.Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers[J].Genetics and Molecular Research：GMR,2014,13(2):3165-3175.[12]FOUGAT R S，PUROHIT A R，KUMAR S，et al.SSR based genetic diversity in Abelmoschus species[J].The Indian Journal of Agricultural Sciences，2015，85(9)：1223-1228.[13]SUSHIL K，MITHIL J P，RANBIR S F，et al.Assessment of genetic diversity among okra genotypes using SSR markers[J].Journal of Plant Biochemistry and Biotechnology，2017，26(2)：172-178.[14]张绪元，黄捷，刘国道.43份黄秋葵种质的ISSR分析[J].热带作物学报，2009，30(3)：293-298.[15]王旭，韩春乐，周亚楠，等.黄秋葵查尔酮合成酶基因AeCHS的克隆与表达分析[J].植物遗传资源学报，2014，15(3)：561-567.[16]SANGWAN R S，TRIPATHI S，SINGH J，et al.De novo sequencing and assembly of Centella asiatica leaf transcriptome for mapping of structural，functional and regulatory genes with special reference to secondary metabolism[J].Gene，2013，525(2)：58-76.[17]李锡花，吴嫚，于霁雯，等.棉花纤维发育早期 RNA-Seq 转录组分析[J].棉花学报,2013，25(3)：189-196.[18]MEGAN J B，WONKEUN P，PHILI J B，et al.RNA-seq transcriptome profiling of upland cotton(Gossypium hirsutum L.) root tissue under water-deficit stress[J].PLoS One，2013，8(12)：721-732.[19]SCHAFLEITNER R，KUMAR S，LIN C Y，et al.The okra (Abelmoschus esculentus) transcriptome as a source for gene sequence information and molecular markers for diversity analysis[J].Gene，2013，517(1)：27-36.[20]张少平，应璐，邱珊莲，等.基于转录组测序的紫色秋葵花青素合成相关基因研究[J].福建农业学报，2018，33(5)：474-480.[21]李和平，姚运法，练冬梅，等.黄秋葵果实转录组测序及分析[J].生物技术通报，2018，34(3)：121-127.[22]彭佳佳.红掌转录组测序及花青素生物合成途径差异表达基因的分析[D].银川:宁夏大学,2015.[23]LI X，BONAWITZ N D，WENG J K，et al.The growth reduction associated with repressed lignin biosynthesis in Arabidopsis thalianais independent of flavonoids[J].Plant Cell，2010，22(5)：1620-1632.[24]杨红丽，王彦昌，姜正旺，等.‘红阳’猕猴桃cDNA文库构建及F3H基因的表达初探[J].遗传，2009，31(12):1265-1272.[25]焦淑珍，刘雅莉，娄倩，等.葡萄风信子二氢黄酮醇4-还原酶基因(DFR)的克隆与表达分析[J].农业生物技术学报，2014，22(5):529-540.[26]殷剑美，王立，张培通，等.山药花青素合成关键酶基因的解析[J].江西农业学报，2018，30(7):1-6.
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备注/Memo
第一作者简介：王俊青（1981-），男，硕士，实验师，现主要从事药用植物资源利用等研究工作。E-mail：173019503@qq.com.责任作者：李艳艳(1984-)，女，博士，副教授，现主要从事药用植物资源利用与分子生物学等研究工作。E-mail：liyanyan041@163.com.基金项目：国家自然科学基金资助项目（31700597）；河南省高等学校重点科研资助项目（22B350005）。收稿日期：2022-01-21