DONG Hua,CHEN Hong.Genetic Difference Analysis of Suspected ‘Fengtang Plum’ Germplasm Based on Leaf Phenotypic Characteristics[J].Northern Horticulture,2022,(15):25-33.[doi:10.11937/bfyy.20220056]
基于叶片表型性状的疑似‘蜂糖李’种质遗传差异分析
- Title:
- Genetic Difference Analysis of Suspected ‘Fengtang Plum’ Germplasm Based on Leaf Phenotypic Characteristics
- Keywords:
- ‘Fengtang Plum’; blade; quality traits; quantitative traits; principal component analysis; correlation analysis; cluster analysis
- 文献标志码:
- A
- 摘要:
- 以贵州75个疑似‘蜂糖李’单株为试材，‘蜂糖李’为对照，对12个叶片表型性状进行分析，探讨不同材料间的遗传差异，以期为‘蜂糖李’品种保护提供参考依据。结果表明：1）75份供试材料中8个质量性状共观测到26个变异类型，Shannon-Wiener信息指数H和Simpson遗传多样性指数D,变化范围分别为0.667 4～1.201 4和0.488 1～0.678 3。4个数量性状变异系数变化范围为8.38%～17.50%，其中叶柄长变异系数最大，为17.50%。2）主成分分析结果显示特征值大于1的主成分共5个，累计贡献率达到67.618%，其中各主成分中因子负载量较大的为叶形指数、叶面状态、叶尖、叶缘和叶柄颜色。3）R型聚类和性状相关性分析结果表明12个叶片表型性状对75个材料进行分类合理。Q型聚类结果显示，在欧式距离为16.0时，将76份材料分为3组，可将 YS60、YS71和YS73 3个材料和YS28、YS31、YS39等9个材料单独分为2组，说明与对照有完全不同的遗传背景。在欧式距离5.0时，可将YS1、YS6、YS75等27个材料与对照区分开，说明该27个材料与对照之间存在较小的遗传差异。
- Abstract:
- Taking ‘Fengtang Plum’ as the contrast and seventy-five single plants of ‘Fengtang Plum’ in Guizhou Province as test material to conduct analysis on twelve leaf phenotypic characteristics and explore the genetic differences among different materials,in order to provide reference for protecting the ‘Fengtang Plum’ variety.The results showed that,1) 26 types of variation were observed for eight qualitative traits in 75 test materials,and the Shannon-Wiener Information Index H and Simpson Genetic Diversity Index D ranged from 0.667 4 to 1.201 4 and 0.488 1 to 0.678 3,respectively.The coefficients of variation for four quantitative traits ranged from 8.38% to 17.50%.Among them,the coefficient of variation of petiole length was the largest,17.50%.2)The results of principal component analysis showed that there were five principal components with eigenvalues greater than 1,and the cumulative contribution rate reached 67.618%.Among them,the factors with higher factor loadings in each principal component were leaf shape index,leaf surface condition,leaf tip,leaf margin and leaf stalk color.3)The results of R-type clustering analysis and correlation analysis of characters showed that the twelve leaf phenotypic characteristics classified the seventy-five materials reasonably.The results of Q-clustering showed that 76 materials were classified into three groups at a Euclidean distance of 16.0,which could classify 3 materials,YS60,YS71 and YS73,and nine materials,YS28,YS31 and YS39,ect.,into 2 separate groups.This indicated a completely different genetic background from the control.At Euclidean distance 5.0,27 materials such as YS1,YS6,and YS75 could be distinguished from the control.This indicated that there were small genetic differences between these 27 materials and the control.
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备注/Memo
第一作者简介：董华（1997-），男，硕士研究生，研究方向为‘蜂糖李’种质资源的鉴定及保存。E-mail:394402620@qq.com.〖JP〗责任作者：陈红（1975-），男，博士，副教授，现主要从事生物技术与果树遗传育种等研究工作。E-mail:957831450@qq.com.基金项目：贵州省科技计划资助项目(黔科合支撑[2022]重点01号)。收稿日期：2022-01-06