[1]孟娟,文庭池,丁勇,等.冬荪的鉴定及不同物质对其菌丝生长的影响[J].北方园艺,2022,(12):114-123.[doi:10.11937/bfyy.20215299]
　MENG Juan,WEN Tingchi,DING Yong,et al.Identification of Phallus dongsun and Effects of Different Substances on Its Mycelial Growth[J].Northern Horticulture,2022,(12):114-123.[doi:10.11937/bfyy.20215299]
点击复制

冬荪的鉴定及不同物质对其菌丝生长的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年12 页码: 114-123 栏目: 出版日期: 2022-06-30
Title:
Identification of Phallus dongsun and Effects of Different Substances on Its Mycelial Growth
作者:
孟娟1; 2; 3; 文庭池2; 3; 丁勇1; 肖沅嫔2; 3; 4; 杨雨2; 3
（1.西南林业大学 云南省高校林木生物技术重点实验室，云南 昆明 650224；2.贵州大学 西南药用生物资源教育部工程研究中心，贵州 贵阳 550025；3.贵州大学 食用菌研究院，贵州 贵阳 550025；4.泰国皇太后大学 真菌研究卓越中心，清莱 泰国 57100）
Author(s):
MENG Juan1; 2; 3; WEN Tingchi2; 3; DING Yong1; XIAO Yuanpin2; 3; 4; YANG Yu2; 3
(1.Key Laboratory of Forest Biotechnology in Yunnan，Southwest Forestry University，Kunming,Yunnan 650224;2.The Engineering Research Center of Southwest Bio-Pharmaceutical Resources Ministry of Education，Guizhou University，Guiyang,Guizhou 550025;3.The Mushroom Research Centre，Guizhou University，Guiyang,Guizhou 550025;4.Center of Excellence in Fungal Research,Mae Fah Luang University,Chiang Rai,Thailand 57100)
关键词:
冬荪; 分子鉴定; 营养生理
Keywords:
Phallus dongsun; molecular identification; nutritional physiology
DOI:
10.11937/bfyy.20215299
文献标志码:
A
摘要:
以采自贵州省龙里县的疑似冬荪菌株为试材，采用ITS序列分析对其进行分子鉴定，并采用单因素试验研究不同物质对其菌丝生长的影响，以期明确其分类学地位，并为冬荪（Phallus dongsun）的人工栽培和后续的深入研究提供参考依据。结果表明：该菌株的ITS序列长576 bp，与冬荪相似度最高，鉴定该菌株为冬荪（Phallus dongsun）；该菌株液体种生长的最佳碳源为果糖，其次为葡萄糖；最佳氮源为酵母浸膏，其次为牛肉膏；栽培种生长的最佳主料为桃树，其次为桦槁树；最佳辅料为甘蔗渣，其次为玉米芯。
Abstract:
The suspected Phallus dongsun strain collected from Longli county，Guizhou Province was used as the test material，and its molecular identification was carried out by ITS sequence analysis，and the influences of different substances on the growth of mycelium were studied by single factor experiments，in order to clarify its taxonomic status，and provide reference for artificial cultivation of Phallus dongsun and follow-up in-depth research.The results showed that the length of the ITS sequence was 576 bp，which had the highest similarity with Phallus dongsun，and the strain was identified as Phallus dongsun.The best carbon source for the growth of the liquid strain of this strain was fructose，followed by glucose;the best nitrogen source was yeast extract，followed by beef extract;the best main material for the growth of cultivar was peach tree，followed by birch tree;and the best auxiliary material was bagasse，followed by corn cob.

参考文献/References:

[1]LI T，LI T，DENG W，et al.Phallus dongsun and P.lutescens，two new species of Phallaceae (Basidiomycota) from China[J].Phytotaxa，2020,443(1):19-37.[2]首坤秀，王山立，林灵，等.冬荪及其蛋托的成分分析和抗氧化活性研究[J].食品科技，2020,45(2):264-271.[3]朱国胜，桂阳，刘鹏，等.白鬼笔仿野生栽培技术[J].农技服务，2018,35(3):36-40.[4]李永荷，肖萧，杨晋，等.简述白鬼笔栽培技术及病虫害防治措施[J].南方农业，2019,13(17):17-18.[5]杨珍.黔产冬荪重金属及农残含量与食用潜在健康风险评价[D].贵阳：贵州师范大学，2016.[6]赵凯，王飞娟，潘薛波，等.红托竹荪菌托多糖的提取及抗肿瘤活性的初步研究[J].菌物学报，2008(2):289-296.[7]KIM B K，CHOI E C，CHUNG K S，et al.Studies on constituents of Korean basidiomycetes L.[J].Archives of Pharmacal Research，1983,6(2):141-142.[8]NIKSIC M，HADZIC I，GLISIC M.Is Phallus impudicus a mycological giant?〖KG-*3〗[J].Mycologist，2004,18(1):21-22.[9]首坤秀.冬荪成分分析、活性评价及其多糖分离纯化和结构性质[D].贵阳：贵州医科大学，2020.[10]KUZNECOVA G，KUZNECOVS I，JEGINA K，et al.8167 POSTER lymphedema prevention and management with Phallus impudicus:Six-years survey after breast cancer treatment[J].European Journal of Cancer Supplements，2007,5(4):457.[11]石联.α-吡咯烷酮的应用与开发进展[J].化工中间体，2003(5):19-21.[12]刘波.中国真菌志.第二十三卷,硬皮马勃目 柄灰包目 鬼笔目 轴灰包目[M].北京：科学出版社，2005.[13]LI H，MA X，MORTIMER P，et al.Phallus haitangensis，a new species of stinkhorn from Yunnan Province，China[J].Phytotaxa，2016,280(2):116-128.[14]CABRAL T S，SILVA B D，MARTN M P，et al.Behind the veil-exploring the diversity in Phallus indusiatus s.l.(Phallomycetidae，Basidiomycota)[J].Myco Keys，2019,58:103-127.[15]LI H，MORTIMER P，KARUNARATHNA S，et al.New species of Phallus from a subtropical forest in Xishuangbanna，China[J].Phytotaxa，2014,163(2):91-103.[16]袁滨，严俊杰，柯丽娜，等.基于ITS系列分析鉴定野生灵芝属菌种[J].中国食用菌，2018,37(2):62-66.[17]SCHOCH C L，SEIFERT K A，HUHNDORF S，et al.Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi[J].Proceedings of the National Academy of Sciences，2012,109(16):6241.[18]聂建军，李彩萍，杨玉画，等.酒红球盖菇液体摇瓶培养基优化[J].山西农业科学，2011,39(10):1073-1075.[19]LANDEWEERT R，LEEFLANG P，KUYPER T W，et al.Molecular identification of ectomycorrhizal mycelium in soil horizons[J].Applied and Environmental Microbiology，2003,69(1):327-333.[20]元玲刚，常明昌，孟俊龙，等.不同碳源对绣球菌菌丝生长的影响[J].山西农业科学，2015,43(8):951-952.[21]余昌霞，李正鹏，赵妍，等.草菇液体菌种培养基配方及培养条件的优化[J].上海农业学报，2020,36(5):41-45.[22]刘晓鹏，姜宁，魏璐，等.白灵菇深层发酵培养基的优化研究[J].中国酿造，2009(3):65-68.[23]刘海娟，刘利娟，郑素月，等.白灵菇液体发酵条件优化[J].北方园艺，2021(18):125-131.[24]赵洪，李静，向旭，等.红汁乳菇菌丝体液体发酵条件的优化研究[J].江苏农业科学，2020,48(14):167-169.[25]徐鸿雁，杜双田，孟胜楠，等.不同碳氮源对红汁乳菇菌丝生长的影响[J].西北农林科技大学学报(自然科学版)，2013,41(10):125-130.[26]陈斌，郭爱珍，赵毅，等.不同氮源对白灵菇菌丝生长的影响[J].山西农业科学，2015,43(1):28-30.[27]马艳弘，张福元，何云峰.竹荪液体培养适期营养需求的研究[J].山西农业大学学报，2001(1):20-23.[28]黄春燕，宫志远，刘路明，等.不同碳氮源对金针菇液体菌种培养的影响[J].山东农业科学，2020,52(11):138-140.

相似文献/References:

[1]刘贵巧,何华奇,王建明.一株野生食用菌株的种类鉴定研究[J].北方园艺,2014,38(12):118.
　LIU Gui-qiao,HE Hua-qi,WANG Jian-ming.Research on Species Identification of a Wild Edible Mushroom[J].Northern Horticulture,2014,38(12):118.
[2]石宝萍,李成亮,都业娟,等.新疆田旋花丛簇病植原体的分子鉴定[J].北方园艺,2013,37(19):114.
　SHI Bao-ping,LI Cheng-liang,DU Ye-juan,et al.Molecular Identification of Phytoplasma Strain Associated with Convolvulus arvensis Cluster Disease in Xinjiang[J].Northern Horticulture,2013,37(12):114.
[3]刘灵娣,谢晓亮,温春秀,等.不同丹参种质的rDNA ITS序列分析[J].北方园艺,2012,36(20):146.
　LIU Ling-di,XIE Xiao-liang,WEN Chun-xiu,et al.rDNA ITS Sequence Analysis of Different Germplasm of Salvia miltiorrhiza Bge[J].Northern Horticulture,2012,36(12):146.
[4]武冬梅,许文涛,谢宗铭,等.新疆伊犁昭苏野生羊肚菌分离株的分子鉴定研究[J].北方园艺,2014,38(21):145.
　WU Dong-mei,XU Wen-tao,XIE Zong-ming,et al.Molecular Identification of Morchella in Xinjiang Yili[J].Northern Horticulture,2014,38(12):145.
[5]成玉梅,赵丹,康业斌.牡丹根腐病病原的形态与分子鉴定[J].北方园艺,2015,39(16):116.[doi:10.11937/bfyy.201516027]
　CHENG Yumei,ZHAO Dan,KANG Yebin.Molecular and Morphological Identification of Peony Root Rot Pathogen[J].Northern Horticulture,2015,39(12):116.[doi:10.11937/bfyy.201516027]
[6]刘主,蔡爱群,刘静华,等.基于ITS区序列的疑似野生“南华”草菇菌株的分子鉴定[J].北方园艺,2016,40(01):126.[doi:10.11937/bfyy.201601033]
　LIU Zhu,CAI Aiqun,LIU Jinghua,et al.Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence[J].Northern Horticulture,2016,40(12):126.[doi:10.11937/bfyy.201601033]
[7]姜雪,庞惟俏,杨洋,等.两株野生紫色香蘑菌株的鉴定[J].北方园艺,2016,40(22):152.[doi:10.11937/bfyy.201622038]
　JIANG Xue,PANG Weiqiao,YANG Yang,et al.Identification of Two Wild Lepista Strains[J].Northern Horticulture,2016,40(12):152.[doi:10.11937/bfyy.201622038]
[8]刘贵巧,王建书,王秋敏,等.血红栓菌的分离鉴定及对牛筋草种子萌发的影响[J].北方园艺,2017,41(04):102.[doi:10.11937/bfyy.201704023]
　LIU Guiqiao,WANG Jianshu,WANG Qiumin,et al.Isolation and Identification of Trametes sanquinea and Its Effect on Seed Germination of Eleusine indica[J].Northern Horticulture,2017,41(12):102.[doi:10.11937/bfyy.201704023]
[9]张剑,付亚娟.承德两种野生食用菌的分子鉴定及系统发育分析[J].北方园艺,2017,41(17):172.[doi:10.11937/bfyy.20170330]
　ZHANG Jian,FU Yajuan.Molecular Identification and Phylogenetic Analysis of Two Wild Edible Mushrooms in Chengde Area[J].Northern Horticulture,2017,41(12):172.[doi:10.11937/bfyy.20170330]
[10]张建瑛,田新华,殷东生,等.毛樱桃与李子杂交新品种的早期鉴定[J].北方园艺,2018,42(06):52.[doi:10.11937/bfyy.20173034]
　ZHANG Jianying,TIAN Xinhua,YIN Dongsheng,et al.Early Identification for New Varieties of Prunus tomentosa Thunb.and  Prunus salicina Lindl.[J].Northern Horticulture,2018,42(12):52.[doi:10.11937/bfyy.20173034]

备注/Memo

第一作者简介：孟娟（1993-），女，硕士研究生，研究方向为微生物生理学。E-mail：1030434341@qq.com.责任作者：丁勇（1979-），男，硕士，副教授，现主要从事生物技术教学与科研等工作。E-mail：dingyong@swfu.edu.cn.基金项目：贵州省科技厅重大专项资助项目（黔科合重大专项字[2019]3005-5号）；云南省高校林木生物技术重点实验室开放基金资助项目（51700201）。收稿日期：2021-12-27

更新日期/Last Update: 2022-08-26