ZHENG Jun,SHAN Cancan,ZHANG Zihua,et al.Effects of CO2 Concentration on Biological Parameters of Post-maturing Stage of Hypsizygus marmoreus[J].Northern Horticulture,2022,(09):115-121.[doi:10.11937/bfyy.20213839]
CO2浓度对真姬菇后熟阶段生物学参数的影响
- Title:
- Effects of CO2 Concentration on Biological Parameters of Post-maturing Stage of Hypsizygus marmoreus
- Keywords:
- Hypsizygus marmoreus bag; CO2 concentration; physical and chemical properties; extracellular enzyme; yield
- 文献标志码:
- A
- 摘要:
- 以真姬菇后熟期菌包（60~120 d）为试材，设定低CO2浓度（0.05%~0.20%）、中CO2浓度（0.35%~0.50%）、高CO2浓度（0.70%~1.00%）3个不同CO2浓度处理，研究了不同CO2浓度培养下菌包上、中、下3个部分的理化性能及胞外酶活性，拟得出最适CO2的浓度范围，以期为工厂化生产真姬菇提供参考依据。结果表明：在中、低CO2浓度范围，菌包上、中、下3个部分的pH、可溶性蛋白质含量、总糖含量以及羧甲基纤维素酶、木聚糖酶、漆酶、锰过氧化物酶、淀粉酶等活性变化趋势大体一致，中CO2浓度培养时，120 d时上、中、下部指标一致性较好，说明菌包成熟度高；而在同等条件下用高CO2浓度培养的菌丝代谢已出现抗逆性紊乱。综上所述，CO2浓度对菌包产量有影响，中等CO2浓度的菌包出菇产量最高（520.40 g?袋-1）,而CO2浓度过低（496.66 g?袋-1）或过高（453.33 g?袋-1）均不利于提高菌包产量。
- Abstract:
- Taking the bacterial bag of Hypsizygus marmoreus in the ripening stage (60-120 days) at test material,three treatments with different CO2 concentrations,low（0.05%-0.20%）、medium（0.35%-0.50%） and high （0.70%-1.00%） level of CO2 concentrations were set,the effects of the CO2 concentration to the upper、middle and lower parts of the package yield were studied,the optimum concentration range of CO2 was drawn up,in order to provide reference for industrialized production of Hypsizygus marmoreus.The results showed that several characters including the pH,the soluble protein and total sugar contents,and the activities of carboxymethyl cellulase,xylanase,laccase,manganese peroxidase,amylase had the same trend,when the concentrations of CO2 were controlled in a medium or low level.When the packet cultivated 120 days under medium concentrations,indicators from the upper,middle and lower parts of package were most consistent than other concentrations.It indicated that maturity of the pack was well.However,under the condition with high concentrations of CO2,the hyphae metabolism had appeared as anti-reverse disorder.Furthermore,the concentration of CO2 had an effect on the yield.The results indicated those incubated with a medium level of CO2 concentration producted the highest yield (520.40 g?bag-1),and other samples performed smaller package yields,no matter under a low (496.66 g?bag-1) or high (453.33 g?bag-1) level of CO2 concentrations.
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备注/Memo
第一作者简介：郑峻（1968-），男，本科，高级农艺师，现主要从事食用菌栽培技术研究及应用推广等工作。E-mail：zhengjun7855075@163.com.责任作者：胡开辉（1962-），男，本科，教授，现主要从事食用菌的应用开发与工厂化栽培等研究工作。E-mail：Hukh@fafu.edu.cn.基金项目：福建农林大学科技创新专项基金资助项目（KFA17321A,KFA17585A）。收稿日期：2021-09-16