SHA Guofei,LU Min,AN Huaming.Effects of EMS Mutagenesis on Rosa roxburghii Tratt.Seed Emergence and Its Early Growth Traits[J].Northern Horticulture,2022,(02):33-39.[doi:10.11937/bfyy.20212413]
EMS诱变对刺梨种子出苗及早期生长性状的影响
- Title:
- Effects of EMS Mutagenesis on Rosa roxburghii Tratt.Seed Emergence and Its Early Growth Traits
- Keywords:
- Rosa roxburghii Tratt.; EMS mutagenesis; growth; seedling rate
- 文献标志码:
- A
- 摘要:
- 以刺梨品种‘贵农5号’的种子为试材，种皮经砂纸磨损后用浓度为0.1%、0.3%、0.6%、0.9%的甲基磺/酸乙酯（EMS）分别浸种处理12 h和24 h后播种，研究了出苗率、成苗率并观测幼苗早期生长表型性状，以期筛选出最适诱变剂量和处理时间，为后期构建大量刺梨EMS突变群体奠定技术基础。结果表明：0.1%～0.3%的低浓度EMS处理能提高种子的出苗率和成苗率，但随着EMS浓度升高或处理时间的延长，出苗率和成苗率均显著降低，当浓度升至0.9%处理12 h或0.6%处理24 h时其种子死亡率为100%。成活幼苗的生长性状可在株高、株型、叶型、分枝等方面表现出不同的表型变异，且在一定浓度范围内其变异率随着处理浓度增加而增加。综合考虑成苗率和表型变异率等数据，筛选得出0.3% EMS浸种12 h为刺梨种子突变的适宜剂量条件。
- Abstract:
- The seeds of Rosa roxburghii Tratt.variety ‘Guinong 5’ were used as materials,the seeds coat were abraded by sandpaper,and then sown after soaked with ethyl methane sulfonate (EMS) with the concentration of 0.1%,0.3%,0.6%,0.9% for 12 hours and 24 hours respectively,the emergence rate and seedling survival rate were counted and the phenotypic characters of early seedling growth were observed,in order to screen out the most suitable mutagenic dose and treatment time and lay a technical foundation for subsequent constructing a large number of EMS mutant populations of Rosa roxburghii Tratt..The results showed that 0.1%-0.3% low concentration EMS improved the emergence rate and seedling rate of seeds,but with the increasing of EMS concentration or the extending of treatment time,both the emergence rate and seedling survival rate decreased significantly.When the concentration rose to 0.9% for 12 hours or 0.6% for 24 hours,the seed mortality rate was 100%.The growth characteristics of living seedlings showed different phenotypic variation in plant height,plant architecture,leaf type and branch,and the variation rate increased with the increase of treatment concentration in a certain concentration range.Based on the data of seedling rate and phenotype variation rate,0.3% EMS soaking for 12 hours was selected as the optimum dosage condition for seed mutation of Rosa roxburghii Tratt. seeds.
参考文献/References:
[1]LIU M H,ZHANG Q,ZHANG Y H,et al.Chemical analysis of dietary constituents in Rosa roxburghii and Rosa sterilis fruits[J].Molecules,2016,21(9):1-21.[2]HE J Y,ZHANG Y H,MA N,et al.Comparative analysis of multiple ingredients in Rosa roxburghii and R sterilis fruits and their antioxidant activities[J].Journal of Functional Foods,2016(27)：29-41.[3]王小平.刺梨(Rosa roxburghii Tratt.)离体繁殖与四倍体选育研究[D].重庆:西南大学,2009.[4]廖安红.60Co-γ射线及化学诱变剂对刺梨诱变效应的研究[D].贵阳:贵州大学,2016.[5]梁任繁,何龙飞.EMS在作物育种应用中的研究进展[J].种子,2008(2)：47-49.[6]臧辉,任卫波.EMS诱变在植物育种中的研究与应用[J].分子植物育种,2018,16(17)：5782-5788.[7]BROCKMAN H E,de SERRES F J,ONG T M,et al.Mutation tests in Neurospora crassa.A report of the U.S.environmental protection agency gene-tox program[J].Mutation research,1984,133(2):87-134.[8]LI Y X,WANG M N,SEE D R,et al.Ethyl-methanesulfonate mutagenesis generated diverse isolates of Puccinia striiformis f.sp.tritici,the wheat stripe rust pathogen[J].Springer Netherlands,2019,35(2)：28.[9]CHEN C,CUI Q Z,HUANG S W,et al.An EMS mutant library for cucumber[J].Journal of Integrative Agriculture,2018,17(7)：1612-1619.[10]RIME J,DINESH M R,SANKARA M,et al.Evaluation and characterization of EMS derived mutant populations in mango[J].Scientia Horticulturae,2019,254:55-60.[11]ESPINA M J,SABBIR A C M,ANGELINA B,et al.Development and phenotypic screening of an ethyl methane sulfonate mutant population in soybean[J].Frontiers in plant science,2018(9):394.[12]MALIATA A W,HUSSEIN S,LYDIA N H,et al.The effect of single and combined use of gamma radiation and ethylmethane sulfonate on early growth parameters in sorghum[J].Plants,2020,9(7):7.[13]SUN J,LU N S,CHEN Z H,et al.Generation and characterization of a foxtail millet (Setariaitalica) mutant library[J].Frontiers in Plant Science,2019(10)：369.[14]MUHAMMAD I S,SEUNGKI B,JOUNG-HO L,et al.Development and characterization of an ethyl methane sulfonate (EMS) induced mutant population in Capsicum annuum L.[J].Plants,2020,9(3)：396-396.[15]JOHANNA L,SHAHRIAR S M S,SAMEER H,et al.Development and characterization of an EMS-mutagenized wheat population and identification of salt-tolerant wheat lines[J].BMC Plant Biology,2020,20(1)：18.[16]TALEBI A B,TALEBI A B,SHAHROKHIFAR B.Ethyl methane sulphonate (EMS) induced mutagenesis in Malaysian rice (cv.MR219) for lethal dose determination[J].American Journal of Plant Sciences,2012,3(12)：1661-1665.[17]ARISHA M H,SHAH S N M,GONG Z H,et al.Ethyl methane sulfonate induced mutations in M2 generation and physiological variations in M1 generation of peppers (Capsicum annuum L.)[J].Frontiers in Plant Science,2015(6)：399-410.[18]SHAH S N M,GONG Z H,ARISHA M H,et al.Effect of ethyl methyl sulfonate concentration and different treatment conditions on germination and seedling growth of the cucumber cultivar Chinese long (9930)[J].Genetics and Molecular Research：GMR,2015,14(1)：2440-2449.[19]PORCH T G,BLAIR M W,LARIGUET P,et al.Generation of a mutant population for TILLING common bean genotype BAT 93[J].Journal of the American Society for Horticultural Science,2009,134(3)：348-355.[20]COOPER J L,TILL B J,LAPORT R G,et al.TILLING to detect induced mutations in soybean[J].Bio Med Central,2008,8(1):9.[21]赵越.EMS诱导小麦突变及优良突变鉴选[D].杨凌:西北农林科技大学,2020.[22]仇婷婷,李瑞梅,周杨骄,等.EMS不同浓度和处理时间对华南5号木薯种子的影响[J].分子植物育种,2019,17(9):2965-2973.[23]云娜,石凤翎,王晓龙,等.EMS诱变对蒙农红豆草出苗和幼苗生长的影响[J].中国草地学报,2014,36(3):5-9.[24]范昕琦,王海燕,聂萌恩,等.EMS诱变对高粱出苗及农艺性状的影响[J].作物杂志,2020(1):47-54.[25]杨建胜,孙万仓,刘自刚,等.EMS诱变对北方白菜型冬油菜农艺性状与品质的影响[J].干旱地区农业研究,2018,36(1):213-220.[26]高营,闫强,袁星星,等.甲基磺酸乙酯诱变对绿豆种子萌发及幼苗生长的影响[J].江苏农业科学,2020,48(16):104-108.[27]黎诗艳,阮景军,范昱,等.EMS诱变剂处理对苦荞种子萌发及主要农艺性状的影响[J].分子植物育种,2021,19(3):914-922.
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备注/Memo
第一作者简介：沙国飞（1995-），女，硕士研究生，研究方向为刺梨育种。E-mail:1822765316@qq.com.责任作者：安华明（1973-），男，博士，教授，现主要从事果树生理学等研究工作。E-mail:anhuaming@hot-mail.com.基金项目：国家自然科学基金资助项目(31660549);贵州省高层次创新型人才培养资助项目（黔科合人才[2016]4016号）。收稿日期：2021-06-07