SONG Yan,GAO Shan,WANG Xiaohai,et al.Response of Nutrient Content and Key Enzyme Activity of Nitrogen Metabolism to Nitrogen Level in Walnut Seedling[J].Northern Horticulture,2022,(01):40-46.[doi:10.11937/bfyy.20212186]
核桃实生苗叶片养分含量及氮代谢关键酶活性对氮素水平的响应
- Title:
- Response of Nutrient Content and Key Enzyme Activity of Nitrogen Metabolism to Nitrogen Level in Walnut Seedling
- Keywords:
- walnut seedling; grow; nitrogen metabolizing enzyme; nutrient elements
- 文献标志码:
- A
- 摘要:
- 以一年生核桃实生苗为试材，设置不同氮处理（N1：5 g·株-1、N2：10 g·株-1、N3：15 g·株-1和N4：20 g·株-1），以清水处理为对照（N0）,研究了核桃实生苗株高、地径、叶片养分含量及氮代谢关键酶活性，以期为核桃实生苗生产过程中科学施肥策略的制定及养分的高效管理提供参考依据。结果表明：不同氮水平处理后核桃实生苗的株高、地径呈先升后降的趋势，阿克苏种源地与和田种源地核桃实生苗的株高、地径分别在N2、N3处理达到峰值。核桃实生苗叶片蛋白质含量及硝酸还原酶活性在不同处理下表现出先升后降的变化趋势，经N2处理后达到峰值，蛋白质含量分别为（A：26.242 7 mg·g-1、H：25.436 0 mg·g-1）、硝酸还原酶活性分别为（A：34.982 4 μg·g-1·h-1、H：34.199 0 μg·g-1·h-1），均显著高于N0；同时，氮素促进谷氨酰胺合成酶活性的升高。氮素促进2个种源地核桃实生苗叶片中N、P、K元素的积累，当施氮量超过N3处理后P元素的积累被抑制，均显著高于对照，分别是对照的1.31倍和2.00倍。因此，氮素在核桃实生苗的生长发育过程中起着关键的作用，适宜的施氮量可促进核桃实生苗的生长、对氮素的同化以及营养元素含量的积累。
- Abstract:
- Taking one-year-old walnut seedlings as the experimental materials,different nitrogen treatments were set as N1:5 g·plant-1,N2:10 g·plant-1,N3:15 g·plant-1 and N4:20 g·plant-1,respectively,and water treatment was used as control.The plant height,ground diameter,leaf nutrient content and key enzyme activities of nitrogen metabolism of walnut seedlings were studied,in order to provide a reference for the formulation of scientific fertilization strategy and efficient nutrient management in the production of walnut seedlings.The results showed that the plant height and ground diameter of walnut seedlings in different nitrogen levels tended to be lifted first and then reduced,and the plant height and ground diameter of walnut seedlings in Aksu provenance and Hotan provenance reached the peak value in N2 and N3,respectively.The protein content and nitrate reductase activity of walnut seedlings under different treatments showed a trend of lifting first and then inhibition,and reached the peak value after N2 treatment.The protein content and nitrate reductase activity of walnut seedlings were (A:26.242 7 mg·g-1,H:25.436 0 mg·g-1) and (A：34.982 4 μg·g-1·h-1,H：34.199 0 μg·g-1·h-1),respectively,which were significantly higher than N0.At the same time,nitrogen promoted the increase of glutamine synthase activity.Nitrogen promoted the accumulation of elements N,P and K in the leaves of walnut seedlings from both provenances,and the accumulation of elements P was inhibited after the nitrogen application amount exceeded N3 treatment,which was significantly higher than that of the control group (1.31 times and 2.00 times,respectively).Therefore,nitrogen played a key role in the growth and development of walnut seedlings,and appropriate nitrogen application rate can promote the growth of walnut seedlings,nitrogen assimilation and nutrient content accumulation.
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备注/Memo
第一作者简介：宋岩（1993-），男，硕士，现主要从事果树优质高效栽培等研究工作。E-mail：cliff_sy@163.com.责任作者：张锐（1979-），女，博士，教授，现主要从事果树高产栽培机理及分子育种等研究工作。E-mail：zhrgsh@163.com.基金项目：国家重点研发计划资助项目（2020YFD1000703）；兵团惠民工程“农业技术辐射带动”资助项目（MSSS201902）；兵团师域(区域)发展创新支持计划资助项目（2017DB001）。收稿日期：2021-10-08