YANG Shuqing,YAN Ningning,HU Shuanhong,et al.Resistance Identification of Pepper Germplasm Resources Against Phytophthora capsici and Drug Sensitivity Analysis in P.capsici[J].Northern Horticulture,2022,(01):53-58.[doi:10.11937/bfyy.20211362]
辣椒抗疫病性鉴定及辣椒疫霉药剂敏感性分析
- Title:
- Resistance Identification of Pepper Germplasm Resources Against Phytophthora capsici and Drug Sensitivity Analysis in P.capsici
- Keywords:
- pepper germplasm resources; Phytophthora capsici; resistance identification; drug sensitivity test
- 文献标志码:
- A
- 摘要:
- 以27份辣椒种质资源为试材,采用游动孢子灌根法进行了抗疫病水平的鉴定,以期筛选抗疫病的辣椒种质资源。结果表明:鉴定为高抗水平的材料共有3份,占供试材料的11%;其中L151的病情指数最低,为2.86,其次为L19,病情指数为5.71。鉴定为抗病水平的材料共有6份,占供试材料的22%,病情指数介于10.00~30.00。中抗材料有3份,占供试材料的11%,其余15份材料均为感病材料,占供试材料的56%。同时,为了评估辣椒疫霉菌对杀菌剂的敏感性,室内条件下测定了杀菌剂氟啶胺和氰霜唑对疫霉菌菌丝生长的抑制率。表明氟啶胺和氰霜唑对7株疫霉菌菌丝生长抑制率的区间范围分别为42.68%~71.85%和1.87%~55.01%;其中氰霜唑对菌株P5的抑制率最低,仅为1.87%,对菌株P1和P2的抑制率也较低,分别为22.38%和37.50%,预示着供试的部分辣椒疫霉菌菌株对氰霜唑存在不同程度的抗药性风险。
- Abstract:
- Twenty-seven pepper germplasm resources were evaluated by the method of irrigating the root though zoospore,in order to screen pepper germplasm resources resistant to P.capsici.The results showed that 3 of 27 pepper lines were highly resistant to P.capsici,which were 11% of the tested lines.The most resistant line was L151 and the disease index was 2.86.The second resistant line was L19 and the disease index was 5.71.Six of 27 lines were resistant against P.capsici,which were 22% of the tested lines.And the disease index varied from 10.00 to 30.00.Fifteen of 27 lines were susceptible to P.capsici,which were 56% of the tested lines.To test the drug sensitivity in P.capsici to fluazinam and cyazofamid,the mycelial growth inhibition rate of 7 P.capsici isolates grown on medium added fluazinam and cyazofamid were measured.The mycelial growth inhibited by fluazinam varying from 42.68% to 71.85%,whereas inhibited by cyazofamid varying from 1.87% to 55.01%.Cyazofamid had the lowest inhibition rate on isolate P5,which was only 1.87%.And on other two isolates P1 and P2,the inhibition rate of cyazofamid was relatively low as well,i.e.22.38% and 37.50%,respectively.The results indicated that part of the tested P.capsici isolates had chemical drug resistance risk against cyazofamid with different level.
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备注/Memo
第一作者简介:杨叔青(1986-),女,博士,副教授,现主要从事植物病理学等研究工作。E-mail:smartshuging@outlook.com.责任作者:赵君(1969-),女,博士,教授,现主要从事植物病理学等研究工作。E-mail:zhaojun@imau.edu.cn.基金项目:国家自然科学基金资助项目(31860495);兵团英才资助项目(3800056)。收稿日期:2021-03-29