LIANG Zicong,FENG Guojun,YANG Xiaoxu,et al.Phenotype Analysis and Preliminary Gene Mapping of nts in Phaseolus vulgaris Dwarf Slim-stem Mutant[J].Northern Horticulture,2021,(18):14-21.[doi:10.11937/bfyy.20211108]
菜豆矮化细茎突变体nts表型分析及基因初步定位
- Title:
- Phenotype Analysis and Preliminary Gene Mapping of nts in Phaseolus vulgaris Dwarf Slim-stem Mutant
- Keywords:
- Phaseolus vulgaris L.; slim-stem; dwarf; mutant; gene mapping
- 文献标志码:
- A
- 摘要:
- 以菜豆品种A18和矮化细茎突变体nts为试材，利用形态学观察和基因定位等方法，探究菜豆茎的生长发育机理和茎矮化细化形成的分子机制，以期初步定位矮化细茎基因。结果表明：nts的株高、主茎粗、顶端茎粗、分枝数、节数、百粒质量等均显著低于野生型；突变体的净光合速率、叶片的气孔导度和蒸腾速率均低于野生型，胞间CO2浓度较野生型增高；随着植株生长，nts的抗氧化物酶（SOD、POD、CAT）活性均较野生型降低，超氧阴离子(O·〖TX--*9〗2)含量较野生型增加。遗传分析结果表明该基因为单基因隐性遗传，并将其初步定位于8号〖JP3〗染色体SSR标记SSR47和SSR65之间，物理距离为574 kb，该区间内共有67个注释基因。
- Abstract:
- Morphological observation and gene mapping were used to investigate the growth and development mechanism of stem and molecular mechanism of dwarf-slim stem formation in Phaseolus vulgaris L. variety A18 and dwarf slim-stem mutant nts,in order to locate the purpose gene.The results showed that the plant height，main stem thickness，top stem thickness，number of branches，number of nodes，and 100-grains weight of nts were significantly lower than those of wild type；the net photosynthetic rate，stomatal conductance of leaf and transpiration rate of the mutant were lower than those of the wild type，and the intercellular CO2 concentration was higher than that of the wild type.As the plant grew，the antioxidant enzyme activities of nts，SOD，POD，and CAT were all reduced compared to the wild type，and the O·〖TX--*9〗2 content increased compared to the wild type.Genetic analysis showed that the gene was inherited in a recessive single gene，and it was initially located between SSR markers SSR47 and SSR65 on chromosome 8.The physical distance was 574 kb，and there were 67 annotated genes in this interval.
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备注/Memo
第一作者简介：梁子聪（1996-），女，硕士研究生，研究方向为寒区作物种质资源创新及利用。E-mail：442835175@qq.com.责任作者：刘大军（1979-），男，博士，副研究员，现主要从事菜豆育种及栽培生理等研究工作。E-mail：jianlongedu@163.com.基金项目：大宗蔬菜优质抗病新品种选育（菜豆品种育种）资助项目（2019ZX16B02）；黑龙江省自然科学基金联合引导资助项目（LH2019C058）。收稿日期：2021-03-16