WEN Na,ZHAO Xiaoguang,CHEN Wei,et al.Characteristics of Soil Enzyme Activities and Microorganism Quantity in Alpine Grasslands With Different Degrees of Desertification[J].Northern Horticulture,2021,(18):88-94.[doi:10.11937/bfyy.20210985]
不同沙化程度高寒草原土壤酶活性及微生物数量特征
- Title:
- Characteristics of Soil Enzyme Activities and Microorganism Quantity in Alpine Grasslands With Different Degrees of Desertification
- Keywords:
- alpine grassland; desertification; soil enzymes; soil microorganisms
- 文献标志码:
- A
- 摘要:
- 以轻度沙化、中度沙化、重度沙化、极度沙化4个沙化程度的高寒草原土壤为研究对象，采用室内测定配方法,分别采集深度为0~10、10~20、20~30 cm的土壤样品，测定不同沙化程度各土层中土壤蔗糖酶、脲酶、过氧化氢酶、蛋白酶活性及土壤细菌、放线菌、真菌数量，并做相关性分析,探究不同沙化程度高寒草原土壤酶活性及微生物数量特征，分析沙化对土壤酶活性及微生物数量的影响，旨在为草原生态系统的稳定发展提供参考依据。结果表明：1）随着沙化程度的加剧，蔗糖酶活性、蛋白酶活性呈减小趋势，脲酶活性呈先增大后减小趋势,过氧化氢酶活性呈先减小后增大趋势。2）细菌、放线菌、真菌数量均随沙化的加剧呈减小趋势。3）同一沙化程度不同土层下，酶活性及微生物数量随土层深度的增加逐渐减小。4）脲酶、过氧化氢酶、蛋白酶与细菌、放线菌间有一定相关性，与真菌无明显相关性。由此可见，高寒草原严重沙化会导致土壤酶活性有所降低,微生物数量急剧下降，土壤质量状况下降。
- Abstract:
- The mild desertification,moderate desertification,severe desertification,and extreme desertification of alpine grassland soil were selected as the research objects.The soil samples,which were collected from 0-10,10-20 cm,and 20-30 cm,respectively,were used to test the soil sucrase activity,urease activity,catalase activity,protease activity and the number of soil bacteria,actinomycetes,and fungi in these soil samples under different degrees of desertification,and did correlation analysis.In order to explore the characteristics of soil enzyme activity and microbial quantity in the alpine grassland with different degrees of desertification,based on these test results,it was analyzed that the soil enzyme activity and microbial quantity affected by desertification.The results showed that,1) as the degree of desertification intensifies,the activities of soil sucrase and protease decreased gradually,the activity of urease increased first and then decreased,and the activity of catalase first decreased and then increased.2) The number of bacteria,actinomycetes,and fungi showed a decreasing trend with the increasing of desertification.3) Under the same degree of desertification,soil enzyme activity and microbial quantity decreased gradually with the increasing of the soil depth.4) Urease activity,catalase activity,and protease activity had a certain correlation with bacteria and actinomycetes,but there was no obvious correlation with fungi.It could be seen that the severe desertification of the alpine grassland lead to a decrease in soil enzyme activity and a sharp decline in the number of microorganisms,soil quality decline.
参考文献/References:
[1]杨树晶,李涛,干友民,等.阿坝牧区草地不同利用方式与程度对植被碳含量的影响[J].草业学报,2014,23(3):325-332.[2]孙鹏.三江源区不同退化程度高寒草原植被高光谱特征研究[D].兰州:甘肃农业大学,2018.[3]秦嘉海,张勇,赵芸晨.祁连山黑河上游不同退化草地土壤理化性质及养分和酶活性的变化规律[J].冰川冻土,2014,36(2):335-346.[4]李愿会,路秋玲.我国旱区造林绿化的战略思考[J].林业资源管理,2020(4):1-6,49.[5]肖玉.青藏高原高寒草原不同退化程度植物群落特征与土壤养分的关系[D].兰州:兰州大学,2016.[7]李亚娟.青藏高原高寒草地对土地利用格局变化的响应[D].兰州:甘肃农业大学,2013.[7]刘莹莹,苏妮尔,赵彩鸿,等.落叶松凋落叶水提液对苗圃土壤微生物数量和土壤酶活性的影响[J].森林工程,2020,36(5):24-33.[8]马源,张德罡,周恒,等.高寒草甸退化对优势物种根际土壤微生物量及酶活性的影响[J].草原与草坪,2019,39(4):44-52.[9]张莉,党军,刘伟,等.高寒草甸连续围封与施肥对土壤微生物群落结构的影响[J].应用生态学报,2012,23(11):3072-3078.[10]王云霞,马军.不同林龄华北落叶松白桦混交林土壤微生物量碳氮含量研究[J].林业资源管理,2018(4):55-60.[11]王新源,马仲武,王小军,等.不同沙化阶段高寒草甸植物群落与表土环境因子的关系[J].生态学报,2020,40(19):6850-6862.[12]马晓静,郭艳菊,张嘉玉,等.宁夏盐池县沙化草地土壤团聚体分异特征[J].草业学报,2020,29(3):27-37.[13]李立新,陈英智.退化演替对高山草地植被和土壤理化特性影响[J].水土保持通报,2015,35(4):40-42，48.[14]贺凤鹏,曾文静,王曌迪,等.温带草原退化对土壤剖面微生物学特征的影响[J].微生物学通报,2016,43(3):702-711.[15]GB 19733-2003.天然草地退化、沙化、盐渍化的分级指标[S].北京:中国标准出版社,2003.[16]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.[17]舒向阳,胡玉福,蒋双龙,等.川西北沙化草地植被群落、土壤有机碳及微生物特征[J].草业学报,2016,25(4):45-54.[18]王玉琴,尹亚丽,李世雄.不同退化程度高寒草甸土壤理化性质及酶活性分析[J].生态环境学报,2019,28(6):1108-1116.[19]吴俐莎,唐杰,罗强,等.若尔盖湿地土壤酶活性和理化性质与微生物关系的研究[J].土壤通报,2012,43(1):52-59.[20]胡雷,王长庭,王根绪,等.三江源区不同退化演替阶段高寒草甸土壤酶活性和微生物群落结构的变化[J].草业学报,2014,23(3):8-19.[21]李以康,韩发,冉飞,等.三江源区高寒草甸退化对土壤养分和土壤酶活性影响的研究[J].中国草地学报,2008(4):51-58.[22]罗冬,王明玖,郑少龙,等.围封对荒漠草原土壤微生物数量及其酶活性的影响[J].生态环境学报,2016,25(5):760-767.[23]蒋永梅,师尚礼,田永亮,等.高寒草地不同退化程度下土壤微生物及土壤酶活性变化特征[J].水土保持学报,2017,31(3):244-249.[24]王婷,花蕊,楚彬,等.高寒草原退化对植物群落及土壤理化性质的影响[J].草原与草坪,2019,39(4):65-71.[25]杨万勤,王开运.土壤酶研究动态与展望[J].应用与环境生物学报,2002,8(5):564-570.[26]红梅,邵新庆,张建全,等.玉树高寒草甸不同利用方式下土壤微生物的特征[J].草地学报,2015,23(1):75-81.[27]WU X,LI Z H,FU B J,et al.Restoration of ecosystem carbon and nitrogen storage and microbial biomass after grazing exclusion in semi-arid grasslands of Inner Mongolia[J].Ecological Engineering,2014,73(11):395-403.[28]谭秋锦,宋同清,彭晚霞,等.谷型喀斯特不同生态系统的土壤微生物数量及生物量特征[J].生态学报,2014,34(12):3302-3310.[29]卢虎,姚拓,曹莉,等.高寒生态脆弱区不同扰动生境土壤微生物数量的变化[J].土壤通报,2013,44(5):1140-1145.[30]岳海梅,张新军,巩文峰,等.林芝地区不同草地土壤微生物区系分析[J].草业科学,2012,29(7):1019-1022.[31]JIA B,WANG Y,XIE Z.Responses of the restrial carbon cycle to drought over China:Modeling sensitivities of the interactive nitrogen and dynamic vegetation[J].Ecological Modelling,2018,368:52-68.[32]白世红,马风云,李树生,等.黄河三角洲不同退化程度人工刺槐林土壤酶活性、养分和微生物相关性研究[J].中国生态农业学报,2012,20(11):1478-1483.
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备注/Memo
第一作者简介：温娜（1998-），女，硕士研究生,研究方向为生态恢复学。E-mail:563769194@qq.com.责任作者：赵晓光（1965-），男，博士，教授，博士生导师,现主要从事矿山环境保护与生态恢复等研究工作。E-mail:898678322@qq.com.基金项目：中国博士后科学基金资助项目（2018M643689）。收稿日期：2021-03-08