LIANG Chunfei,SHI Guanqi,JIN Xiaoling.Response of Salt Stress on Growth and Physiological of Ilex Cultivars[J].Northern Horticulture,2021,(16):74-82.[doi:10.11937/bfyy.20210234]
不同冬青品种对盐胁迫的生长及生理响应
- Title:
- Response of Salt Stress on Growth and Physiological of Ilex Cultivars
- Keywords:
- Ilex cultivars; leaf salinity index; relative conductivity; malondialdehyde content; soluble protein content; chlorophyll content
- 文献标志码:
- A
- 摘要:
- 以7个冬青品种为试材，采用温室控盆法，研究了不同浓度NaCl（(0、3.0、6.0、9.0 g?L-1）胁迫对其生长和生理影响，并运用隶属函数法综合评价冬青品种NaCl胁迫下的耐受性强弱，以期为冬青品种适生区域的确定提供参考依据。结果表明：1）冬青的盐害症状、死亡率和株高生长与胁迫天数和胁迫浓度显著相关；品种之间存在显著差异。2）相对电导率（Rce）和丙二醛（MDA）含量均与胁迫天数和胁迫浓度呈正相关；超氧化物歧化酶（SOD）活性和可溶性蛋白质含量均与胁迫天数和浓度呈负相关；而过氧化物酶（POD）活性除‘Blue Maid’，与胁迫天数呈正相关外，其它6个品种均随着胁迫天数的延长和胁迫浓度的提高呈先上升后下降的趋势；叶绿素含量(SPAD)则随着胁迫天数的延长呈逐渐下降的趋势。3）叶片盐害指数、Rec、MDA、可溶性蛋白质含量可作为冬青品种耐NaCl胁迫强弱的综合评价指标。4）隶属函数法综合评价结果，耐NaCl胁迫的强弱为‘Blue Maid’>‘Emily Bruner’>‘Tensaw’>‘Anna′s Choice’>‘Mary Nell’>‘Savannah’>‘Forstery’。
- Abstract:
- Seven Ilex cultivars were used as test materials.The influence of different concentrations of NaCl (0 g?L-1，3.0 g?L-1，6.0 g?L-1 and 9.0 g?L-1) for 50 days on the growth and physiological characteristics of two-year-old cuttage seedlings of seven Ilex cultivars were studied by pot culture in the plastic greenhouse，and membership function method was used to evaluate the tolerance of Ilex cultivars under NaCl stress,in order to provide a reference for determining the suitable area of the holly variety.The results showed that,1) Symptom,mortality and plant height growth of salt injury to Ilex cultivars were significantly correlated with stress duration and stress intensity.There were significant differences among Ilex cultivars.2) The Rec and MDA increased with the stress time and the stress concentration.SOD activity and soluble protein content increased at first and then decreased with the extension of stress days.The activity of POD increased at first and then decreased in the other six cultivars except ‘Blue Maid’ which increased continuously with the extension of stress days.SPAD decreased gradually with the extension of stress.3) The contribution rate and weight value of leaf salt injury index,Rec,MDA,soluble protein content and SPAD were higher,which could be used as the comprehensive evaluation index for the identification of seven Ilex cultivars resistant to NaCl stress.4) The comprehensive evaluation results of the membership function method showed that the degree of resistance to NaCl stress was as follows,‘Blue Maid’ >‘Emily Bruner’> ‘Tensaw’ >‘Anna′s Choice’> ‘Mary Nell’> ‘Savannah’ >‘Forstery’.
参考文献/References:
[1]林双冀,孙明.盐胁迫下芙蓉菊与4种菊属植物生理响应特征及其耐盐机理分析[J].西北植物学报,2017,37(6):1137-1144．[2]张建锋,李吉跃,宋玉民,等.植物耐盐机理与耐盐植物选育研究进展[J].世界林业研究,2003,16(2):16-22.[3]王佺珍,刘倩,高娅妮,等.植物对盐碱胁迫的响应机制研究进展[J].生态学报,2017,37(16):5565-5577.[4]李彬,王志春,孙志高,等.中国盐碱地资源与可持续利用研究[J].干旱地区农业研究,2005,23(2):154-158.[5]张磊,侯云鹏,王立春.盐碱胁迫对植物的影响及提高植物耐盐碱性的方法[J].东北农业科学,2018,43(4):11-16.[6]汪晓丽.大叶榉新品种‘恨天高’对盐碱胁迫的生理生化响应[D].长沙：中南林业科技大学,2016.[7]伍江波,金晓玲,汪晓丽,等.盐胁迫对杜仲幼苗生理特征的影响[J].广东农业科学,2015,42(2):17-21.[8]公绪云,周丽霞,傅声雷,等.不同处理对梅叶冬青插穗生根的影响[J].经济林研究,2017,35(3):85-92.[9]胡爱双,肖丹丹,张小栋,等.盐胁迫对2个八棱海棠株系生长与光合荧光特性的影响[J].经济林研究,2020,38(3):208-215.[10]钱燕萍,田如男.冬青属种质资源及其园林应用研究进展[J].世界林业研究,2016,29(3):40-45.[11]惠梓航,姜卫兵,魏家星,等.“冬青”类树种的园林特性及其应用[J].江西农业学报,2011,23(2):46-49.[12]孙晓刚,张雪,刘晓嘉,等.NaCl胁迫对水培‘吉美’海棠生理特性的影响[J].经济林研究,2019,37(4):27-33.[13]金晓玲,傅建敏,张冬林,等.杂交冬青的适应性及其扦插繁殖技术研究[J].中南林业科技大学学报,2012,32(3):7-10.[14]陈慧玲,张新叶,陈华超,等.北美冬青硬枝扦插技术研究初探[J].农学学报,2015,5(9):100-103.[15]曾雯.11个常绿冬青栽培品种的抗寒性研究[D].长沙：中南林业科技大学,2016.[16]金晓玲,汪晓丽,张亚平,等.NaCl胁迫对‘恨天高’榉树生长及生理化特性的研究[M]//张启翔.中国观赏园艺研究进展.北京：中国林业出版社，2017.[17]吴薇.新美柳的盐胁迫响应及外源NO处理的调节作用[D].南京:南京林业大学,2008.[18]王昊伟,杨玲,鲁强,等.盐胁迫对大花四照花种子萌发与幼苗生长的影响[J].南京林业大学学报(自然科学版),2020,44(3):89-94.[19]张磊,侯云鹏,王立春.盐碱胁迫对植物的影响及提高植物耐盐碱性的方法[J].东北农业科学,2018,43(4):11-16.[20]邢建宏,潘德灼,谭芳林,等.NaCl胁迫对秋茄根系渗透调节物质含量的影响[J].生态环境学报,2017,26(11):1865-1871[21]傅聿青,贾漫丽,王军,等.NaCl胁迫下2个桑树品种的脯氨酸、可溶性糖、可溶性蛋白含量变化研究[J].林业与生态科学,2018,33(3):306-310,321.[22]王磊,赵鹏飞,逯久幸,等.茶用菊品种金丝皇菊对盐胁迫的响应[J].河南农业科学,2019,48(2):112-119.[23]潘璐,邹旭,张往祥,等.盐胁迫对两种海棠生长和生理特性的影响[J].东北林业大学学报,2020,48(6):25-31.[24]董建梅,刘翠玉,赵玉洁,等.盐胁迫对石榴生长与生理生化特性的影响[J].西南林业大学学报(自然科学),2021,41(1):1-8.[25]贾漫丽,李娜,李季生,等.‘冀桑2号’嫁接苗对NaCl胁迫的生理响应[J].浙江林业科技,2018,38(4):21-27.[26]郭金博,施钦,熊豫武,等.盐碱混合胁迫对‘中山杉406’生长及光合特性的影响[J].南京林业大学学报(自然科学版),2019,62(1):61-68.[27]史滟滪，杨静慧,左凤月,等.盐胁迫对3种白刺种子萌发的影响及其耐盐性比较[J].河南农业科学,2014,43(9):124-128.
相似文献/References:
[1]陈佳楠,袁小环,刘艳芬.十四种地被植物的耐盐性评价[J].北方园艺,2015,39(22):74.[doi:10.11937/bfyy.201522020]
CHEN Jianan,YUAN Xiaohuan,LIU Yanfen.Assessment of Salt Tolerance of Fourteen Species of Ground Cover Plant[J].Northern Horticulture,2015,39(16):74.[doi:10.11937/bfyy.201522020]
[2]韩志平,郭世荣,王其传.盐胁迫对小型西瓜幼苗生长的影响[J].北方园艺,2016,40(07):13.[doi:10.11937/bfyy.201607004]
HAN Zhiping,GUO Shirong,WANG Qichuan.Effect of Salinity on the Growth of Mini-watermelon Seedlings[J].Northern Horticulture,2016,40(16):13.[doi:10.11937/bfyy.201607004]
[3]姚玉涛,张国新,丁守鹏,等.盐胁迫对草莓苗期生长及氧化还原系统的影响[J].北方园艺,2021,(17):22.[doi:10.11937/bfyy.20210368]
YAO Yutao,ZHANG Guoxin,DING Shoupeng,et al.Effects of Salt Stress on Strawberry Seedling Growth and Antioxidant System[J].Northern Horticulture,2021,(16):22.[doi:10.11937/bfyy.20210368]
备注/Memo
第一作者简介：梁春飞（1987-），男，硕士，副教授，现主要从事风景园林规划设计、园林植物与应用等研究工作。E-mail：357553050@qq.com.责任作者：金晓玲（1963-），女，博士，教授，现主要从事园林植物资源与应用等研究工作。E-mail：121191638@qq.com.基金项目：2020年度广西高等教育本科教学改革工程资助项目（2020JGA375）；国家林草局948资助项目（2008-4-14）。收稿日期：2021-01-16