HE Zidian,GAO Yufeng,YE Xueling,et al.Effects of Trichoderma Phosphate Solubilizing on the Pepper Growth[J].Northern Horticulture,2021,(13):62-68.[doi:10.11937/bfyy.20204107]
溶磷木霉菌对辣椒促生效果
- Title:
- Effects of Trichoderma Phosphate Solubilizing on the Pepper Growth
- Keywords:
- phosphate solubilizer Trichoderma; Trichoderma pseudokoningii; pepper rhizosphere nutrients; growth promoting effect
- 文献标志码:
- A
- 摘要:
- 以Ca3(PO4)2为试材,采用溶磷圈法筛选木霉溶磷菌;采用形态学特征和ITS rDNA序列进行木霉溶磷菌鉴定;将溶磷木霉菌接种至辣椒幼苗根部后,采用常规试验方法测定溶磷木霉菌对辣椒生长性状、品质及根际土壤养分的影响,以期达到用溶磷木霉菌促进辣椒根际土中难溶性磷转化成速效性磷,减少磷肥施用量的目的。结果表明:溶磷木霉菌tang-10-11为拟康氏木霉(Trichoderma pseudokoningii),其溶磷透明圈直径为3.72 cm,显著高于其它木霉菌株。用100倍、300倍、500倍溶磷木霉菌tang-10-11处理后的辣椒根际速效磷含量分别为45.23、50.47、44.00 mg·kg-1,均显著高于清水对照。tang-10-11不仅可以提高辣椒产量,还可以提高辣椒维生素C含量和可溶性糖含量。因此,溶磷木霉菌tang-10-11可以促进辣椒根际土壤难溶性磷转化成速效性磷,从而促进辣椒植株生长,提高产量和品质。
- Abstract:
- Phosphorus-dissolving Trichoderma strains were screened from insoluble inorganic phosphorus media containing Ca3(PO4)2,which as a kind of phosphorus-dissolving material, and identified by morphological characteristics and ITS rDNA sequence. The phosphorus-dissolving ability of the strains was determined by the halo zone methods, and the growth-promoting ability on growth traits, quality and rhizosphere soil nutrients of pepper of the Trichoderma strains were analyzed with conventional method in order to promote the transformation of insoluble phosphorus into available phosphorus in pepper rhizosphere soil by Trichoderma phosphate solubilizing and reduce the application amount of phosphorus fertilizer. was identified as Trichoderma pseudokoningii,and phosphorus transparent circle diameter was 3.72 cm,which was significantly higher than others.Available P contents in the rhizosphere of pepper treated with 100 times,300 times and 500 times of tang-10-11 were 45.23 mg·kg-1,50.47 mg·kg-1 and 44.00 mg·kg-1,respectively,which were significantly higher than those of control.Moreover,tang-10-11 could not only increase the pepper yield but also increase the vitamin C content and soluble sugar content.It can be concluded that phosphate solubilizing Trichoderma tang-10-11 could promote the transformation of insoluble P into available P in the pepper rhizosphere soil and promote pepper growth,increase pepper yield and quality.
参考文献/References:
[1]WANG H,APPAN A,GULLIVER J S.Modeling of phosphorus dynamics in aquatic sediments:II-examination of model perfor-mance[J].Water Research,2003,37(16):3939-3953.[2]赵小蓉,林启美.微生物解磷的研究进展[J].土壤肥料,2001(3):7-11.[3]盛荣,肖和艾,谭周进,等.土壤解磷微生物及其磷素有效性转化机理研究进展[J].土壤通报,2010,41(6):1505-1510.[4]林启美,赵小蓉,孙焱鑫,等.四种不同生态系统的土壤解磷细菌数量及种群分布[J].土壤与环境,2000,9(1):34-37.[5]邵锴,邱业先,徐婧.高效溶磷菌的筛选、鉴定及其溶磷特性[J].江苏农业科学,2017,45(8):253-25.[6]万水霞,王静,李帆,等.玉米根际高效溶磷菌的筛选、鉴定及促生效应研究[J].生物技术通报,2020,36(5):98-103.[7]王同,孔令雅,焦加国,等.红壤溶磷菌的筛选及溶磷机制[J].土壤学报,2014,51(2):373-380.[8]彭艳,孙鑫,周培富,等.贵州两处茶园溶磷青霉菌的筛选、鉴定及溶磷能力分析[J].生态科学,2020,39(3):113-121.[9]孙冉,张素,吴臣林,等.黑曲霉解磷能力的影响因素及培养条件优化[J].应用生态学报,2020,31(6):1963-1970.[10]贺字典,武春成,沈江洁,等.棘孢木霉菌肥对黄瓜枯萎病的防治效果及对连作黄瓜根际土壤微生物种群的影响[J].植物保护学报,2018,45(3):528-535.[11]贺字典,吴素霞,宋晓飞,等.生防菌与茄病镰刀菌在黄瓜根际动态变化[J].中国生物防治学报,2016,32(3):357-364.[12]ALTOMARE C,NORVELL W A,BJORKMAN T.Solubilization of phosphates and micronutrients by the plant growth promoting and biocontrol fungus Trichoderma harzianum Rifai[J].Applied and Environmental Microbiology,1999,65(7):2926-2933.[13]RUDRESH D L,SHIVAPRAKASH M K,PRASAD R D.Tricalcium phosphate solubilizing abilities of Trichoderma spp.in relation to P uptake and growth and yield parameters of chickpea(Cicer arietinum L.)[J].Canadian Journal of Microbiology,2005,51(3):217-222.[14]菅丽萍,刘力行,陈云鹏,等.木霉菌REMI转化子解磷筛选及其解磷效果分析[J].安徽农业科学,2007,35(20):6179-6191.[15]杨合同,于雪云,魏艳丽,等.多功能植病生防木霉的分离筛选与鉴定[J].山东科学,2009,22(5):27-30.[16]阎树刚,韩涛.果蔬及其制品中维生素C测定方法的评价[J].中国农学通报,2002,18(4):110-112.[17]孙清华,张青,刘建文,等.酸水解:斐林试剂法粗淀粉检测最适酸碱度探究[J].酿酒,2015(2):15-21.[18]闫芳,王向岩,彭伟.混合指示剂滴定法测定酸牛乳中酸度的探讨[J].中国公共卫生,1999,15(2):101. [19]杜淼鑫.磷肥水平对设施栽培辣椒生长生理及养分吸收利用的影响[D].兰州:甘肃农业大学,2019.[20]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000.[21]杨榕,王敬敬,徐松,等.溶磷真菌的筛选及耐盐特性分析[J].微生物学通报,2018,45(10):2142-2151.[22]薛应钰,叶巍,杨树,等.高效溶磷木霉菌株复合诱变选育及促生作用[J].核农学报,2018,32(12):2289-2297.[23]薛应钰,叶巍,张树武,等.紫外诱变选育木霉高效解磷菌株[J].核农学报,2015,29(8):1509-1516.[24]赖鉴添,杨婷,史发超,等.蔗叶堆肥中一株泡盛曲霉溶磷能力的鉴定及其对辣椒的促生效果[J].微生物学报,2021,61(1):77-91.[25]RACHANA J,JYOTI S ,VINAY S.Effect of phosphate-solubilizing fungi Aspergillus awamori S29 on mungbean (Vigna radiata cv. RMG 492) growth[J].Folia Microbiologica,2012,57(3):533-541.[26]韩亚杰,李玉娇,吕晓晨,等.溶磷菌组合WF542的筛选及多菌剂联合对辣椒的促生作用[J].食品工业科技,2020,41(24):100-105,112.
备注/Memo
第一作者简介:贺字典(1972-),女,博士,教授,现主要从事蔬菜和中草药病害生物防治等研究工作。E-mail:zidianhe1972@163.com.责任作者:齐红岩(1971-),女,博士,教授,现主要从事设施蔬菜栽培生理学等研究工作。E-mail:qihongyan@syau.edu.cn.基金项目:国家重点研发计划资助项目(2018YFD0201206);河北省重点研发计划资助项目(18226507D);河北省自然科学基金资助项目(C2019407103);河北省高等学校科学技术研究重点资助项目(ZD2019018)。收稿日期:2020-09-23