GENG Xiaojin,JIANG Qiumeng,ZHANG Tengyang,et al.Molecular Identification and Phylogenetic Analysis of Three Wild Edible Mushrooms in Qinhuangdao Area[J].Northern Horticulture,2021,(08):111-116.[doi:10.11937/bfyy.20203174]
秦皇岛地区三种野生食用菌分子鉴定与系统发育分析
- Title:
- Molecular Identification and Phylogenetic Analysis of Three Wild Edible Mushrooms in Qinhuangdao Area
- 文献标志码:
- A
- 摘要:
- （1.廊坊师范学院 生命科学学院，河北 廊坊 065000；2.河北省食药用菌资源高值利用技术创新中心，河北省高校食药用菌资源开发应用技术研发中心，河北 廊坊 065000）
- Abstract:
- In order to lay a foundation for the rational development and artificial domestication of wild rou mushroom,ga mushroom and song mushroom in Qinhuangdao area,the ITS regions of rDNA genes were amplified using ITS1 and TIS4 as primers,cloned and sequenced.ITS sequences were analyzed via NCBI blast program,meanwhile phylogenetic tree were constructed,respectively，in order to provide a foundation for the rational development and artificial domestication of the three wild edible mushrooms.The results showed that rou mushroom had 99% similarity with Chroogompus rutilus (MG4578511),the similarity between ga mushroom and Lactarius akahatsu (EF6850451) was up to 100%,but song mushroom merely had 96% similarity with Suillus granulatus(MF4211071).Phylogenetic analysis showed that there was a close genetic relationship between rou mushroom and C.rutilus,their genetic distance reached to 0.Ga mushroom and L.akahatsu also had higher relationship,the genetic distance between them was 0.Song mushroom had a lower genetic relationship to Suillus granulatus (0033).Therefore,rou mushroom and ga mushroom in Qinhuangdao area were at the molecular level identified as C.rutilus and L.akahatsu,respectively.Song mushroom,which was another kind of wild edible fungi in Qinhuangdao area,belonged to Suillus.
参考文献/References:
[1]暴增海.河北省冀东地区大型真菌资源[J].中国野生植物资源,2000(2):16-18.[2]赵佳浩,冯莎莎,忻龙祚.河北省食用菌野生栽培发展建议[J].南方农业,2019,13(8):128-130.[3]陈青君,牛玉蓉,郝册,等.北京山区油松林地菌根性食用菌发生规律[J].中国农业科学,2011,44(16):3377-3385.[4]董化研,郝册,牛玉蓉,等.血红铆钉菇生态学初步研究[J].中国农学通报,2010,26(7):191-194.[5]刘晓婷,郭九峰,王淑妍,等.用rDNA-ITS方法鉴别内蒙古多种野生食用菌[J].食药用菌,2015,23(5):301-306.[6]WHITE T J,BRUNS T,LEE S.PCR protocols:A guide to methods and applications[M].New York:Academic Press Inc,1990.[7]张文泉,罗国涛,姚庆智.雷公山国家级自然保护区棉革菌属大型真菌形态描述及分子鉴定[J].中南林业科技大学学报,2019,39(6):1-8,15.[8]张家晨,贾福晨,罗章,等.基于ITS序列对林芝市售七种野生大型食用真菌的鉴定[J].北方园艺,2020(8):122-129.[9]LI Y C,YANG Z L,TOLGOR B.Phylogenetic,biogeographic relationships of Chroogomphus species as inferred from molecular,morphological data[J].Fungal Diversity,2009,38:85-104.[10]RENSKE L,PAULA L,THOM W K,et al.Molecular identification of ectomycorrhizal mycelium in soil horizons[J].Applied and Environmental Microbiology,2003,69(1):327-333.[11]戴玉成,周丽伟,杨祝良,等.中国食用菌名录[J].菌物学报,2010,29(1):1-21.[12]SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(16):6241-6246. [13]田慧敏,张亚男,李晓东,等.内蒙古五个环柄菇形态学及rDNA-ITS鉴定[J].食用菌,2019,41(2):21-27.[14]王锋尖,周向宇,甘露.一株野生食用菌的形态及分子鉴定[J].北方园艺,2017(21):159-162.[15]张倢,康曼,韩建荣.一株野生蘑菇的鉴定[J].山西农业科学,2015,43(11):1421-1423,1446.[16]刘东,赵敏.一种野生羊肚菌的鉴定与不同部位菌丝分离保藏[J].林业科技,2016,41(3):27-29.[17]韩鹏,陈青君,贺国强,等.2种野生羊肚菌分离、鉴定与菌丝培养条件[J].北京农学院学报,2018,33(1):37-42.[18]孙军德,丛日辉.血红铆钉菇SN菌株生物学特性研究[J].菌物研究,2011,9(2):105-109.[19]初洋,倪新江,姜明媛,等.浅橙红乳菇的解剖学性状及母种培养基筛选[J].北方园艺,2012(23):165-167.
备注/Memo
第一作者简介：耿晓进（1986-），女，硕士，助理研究员，现主要从事菌根生物学等研究工作。E-mail:gxj0315@sina.com.基金项目：河北省省属高校基本科研业务费资助项目（JQ201906）；廊坊师范学院大学生创新创业训练计划资助项目（Y202010100005）。收稿日期：2020-07-27