ZHANG Xiaoyan,LI Lianguo,GUO Jinli,et al.Comparison and Analysis of Isolation Methods of Photosynthetic Pigment of Leaves in Cerasus humilis[J].Northern Horticulture,2021,(09):104-110.[doi:10.11937/bfyy.20202248]
欧李叶片光合色素提取方法的比较分析
- Title:
- Comparison and Analysis of Isolation Methods of Photosynthetic Pigment of Leaves in Cerasus humilis
- Keywords:
- Cerasus humilis; photosynthetic pigment; extraction
- 文献标志码:
- A
- 摘要:
- 以‘宁欧’、蒙原欧李和‘农大7号’3个抗旱性不同的欧李叶片为试材，通过比较分析4种提取方式6种提取溶剂对叶绿素a、叶绿素b和类胡萝卜素3种光合色素的提取效果，筛选出适合欧李叶片光合色素提取的最佳方案，以期为欧李光合生理、品种选育及抗性生理等方面的研究提供理论参考依据。结果表明：4种提取方法中，研磨法提取效率最高，优选提取试剂C（丙酮∶乙醇=1∶1）；干样浸提法效率低，但受品种影响小，优选提取试剂B（95%乙醇）；鲜样浸提法提取效率较高，但受品种抗性影响较大。液氮法在保存和研磨过程中色素发生降解，含量较低。
- Abstract:
- ‘Ningou’，Mengyuan and ‘Nongda No.7’ were used as experimental materials，and the extraction effects of six extraction solvents under four extraction methods on photosynthetic pigments including chlorophyll a，chlorophyll b and carotenoid were compared and analyzed，the optimal extraction scheme for photosynthetic pigment in leaves of three varieties of Chinese dwarf cherry was screened out,in order to provide a reference for the study of photosynthetic physiology，variety breeding and resistance physiology of Cerasus humilis.The results showed that among the four extraction methods，grinding method had the highest extraction efficiency，and the optimum extraction reagent C (acetone∶ethanol 1∶1) was selected.The dry sample extraction method had low efficiency，but it was less affected by the variety.The optimum extraction reagent B (95% ethanol) was selected.The extraction efficiency of fresh sample extraction method was higher，but it was influenced by the resistance of varieties.The pigment was degraded under liquid nitrogen method during storage and grinding，and the content was low.
参考文献/References:
[1]王红,岳堃,杨成坤,等.UV-B辐射增强处理抑制杧果叶片光合作用的生理原因分析[J].园艺学报,2020,47(2):242-252.[2]刘红梅,周新跃,刘建丰,等.籼型杂交稻光合特性的配合力分析[J].植物遗传资源学报,2014,15(4):699-705.[3]乌凤章.三个越橘品种对盐胁迫的生长和生理响应及耐盐性差异[J].植物生理学报,2019,55(11):1638-1646.[4]叶旺敏,熊德成,杨智杰,等.模拟增温对杉木幼树生长和光合特性的影响[J].生态学报,2019,39(7):2501-2509.[5]马碧花,蔺伟虎,高敏,等.干旱胁迫下水杨酸和内生真菌对多年生黑麦草的影响[J].草业学报,2020,29(1):135-144.[6]李得孝,郭月霞,员海燕,等.玉米叶绿素含量测定方法研究[J].中国农学通报,2005(6):153-155.[7]WELLBURN A R.The spectral determination of chlorophylls a and b，as well as total carotenoids，using various solvents with spectrophotometers of different resolution[J].Journal of Plant Physiology，1994,144(3)：307-313.[8]盛璐,孙梦娇,张茜茹,等.铁线莲‘风之森林’叶绿素提取方法的比较研究[J].赤峰学院学报(自然科学版),2019,35(9):40-43.[9]周武先,段媛媛,卢超,等.高效提取3种不同类型植物叶片色素的方法[J].西北农业学报,2019,28(1):97-104.[10]刘彩云.提取方法、试剂对不同高等植物叶片叶绿素提取效果的比较分析[J].潍坊学院学报,2014,14(2):74-76.[11]卢建男,张琼,刘铁军,等.不同改良剂对盐碱地土壤及草地早熟禾生长的影响[J].草业科学,2017,34(6):1141-1148.[12]马风丽,杜艳民,王阳,等.1-MCP对‘玉露香’梨采后果实品质和叶绿素保持的影响[J].园艺学报,2019,46(12):2299-2308.[13]刘新星,罗俊杰.豌豆幼苗在盐胁迫下的生理生态响应[J].草业科学,2010,27(7):88-93.[14]张宇斌,张永兰,蹇毅,等.不同提取液对蓝莓叶绿素提取效果的影响[J].贵州农业科学,2013,41(2):45-46,49.[15]昌梦雨,魏晓楠,王秋悦,等.植物叶绿素含量不同提取方法的比较研究[J].中国农学通报,2016,32(27):177-180.[16]张素霞.菠菜叶中叶绿素提取工艺研究[J].中国食物与营养,2008(5):40-43.[17]程贵文,龚洪恩,颜送宝,等.油茶叶绿素提取方法的比较研究[J].湖北林业科技,2017,46(6):11-13,58.[18]周丽珠,谷瑶,曾永明,等.香茅草叶绿素含量分析[J].安徽农业科学,2018,46(10):170-171,178.[19]王凤婷,张鑫生,潘洪玉,等.蔬菜叶绿素不同提取方法的比较[J].安徽农业科学,2020,48(6):1-3,6.
相似文献/References:
[1]董晓勤,周 鹏,仇宗浩,等.两个品种绿皮梨及其红色芽变品种光合生理特性的比较[J].北方园艺,2013,37(20):1.
DONG Xiao-qin,ZHOU Peng,QIU Zong-hao,et al.Comparison of Photosynthetic Physiological Characteristics of Two Varieties of Green Pears and Their Red Mutations Varieties[J].Northern Horticulture,2013,37(09):1.
[2]刘显臣,苗金友,柳明玉,等.粒状钙镁磷肥对长白山欧李产量和品质的影响[J].北方园艺,2013,37(02):171.
LIU Xian-chen,MIAO Jin-you,LIU Ming-yu,et al.Effects of GFMP on Yield and Quality of Hybrid Seedlings of Prunus humilis[J].Northern Horticulture,2013,37(09):171.
[3]车家骧,谢元贵,彭熙,等.欧李叶片秋季光合作用日变化研究[J].北方园艺,2012,36(09):6.
CHE Jia-xiang,XIE Yuan-gui,PENG Xi,et al.Study on Photosynthetic Diurnal Variation in Leaves of Cerasus humilis (Bunge) Sok.in Autumn[J].Northern Horticulture,2012,36(09):6.
[4]王添民,惠竹梅.不同形态氮素配比对“赤霞珠”幼苗叶片光合色素与碳水化合物含量的影响[J].北方园艺,2014,38(22):18.
WANG Tian-min,XI Zhu-mei.Effect of Different Nitrogen Form on Photosynthetic Pigment and Carbohydrate Content in Vitis vinifera cv.‘Cabernet Sauvignon’ Leaves[J].Northern Horticulture,2014,38(09):18.
[5]杨文,于泽源,李兴国.三个草莓品种光合特性的研究[J].北方园艺,2015,39(10):50.[doi:10.11937/bfyy.201510011]
YANG Wen,YU Ze-yuan,LI Xing-guo.Study on the Photosynthetic Characteristics of Three Strawberry Cultivars[J].Northern Horticulture,2015,39(09):50.[doi:10.11937/bfyy.201510011]
[6]梁伟玲,卢彦琦,陈翠果,等.欧李优系试管苗增殖关键技术研究[J].北方园艺,2016,40(17):103.[doi:10.11937/bfyy.201617024]
LIANG Weiling,LU Yanqi,CHEN Cuiguo,et al.Key Proliferation Technology of Prunus humilis Fine Strains Plantlets in vitro[J].Northern Horticulture,2016,40(09):103.[doi:10.11937/bfyy.201617024]
[7]肖 啸,张立彬,刘建珍.欧李嫁接成活率的比较研究[J].北方园艺,2011,35(03):0.[doi:10.11937/bfyy.201103012]
XIAO Xiao,ZHANG Li-bin,LIU Jianzhen.of Survival Rate of Grafting in Chinese Dwarf Cherry[J].Northern Horticulture,2011,35(09):0.[doi:10.11937/bfyy.201103012]
[8]焦 淑 华,林 丽 华,李 宝 江.欧李绿枝组织培养与快速繁殖研究[J].北方园艺,2006,30(03):0.[doi:10.11937/bfyy.200603081]
[J].Northern Horticulture,2006,30(09):0.[doi:10.11937/bfyy.200603081]
[9]哈小丽,韩占江,梁继业.不同欧李品种（系）光合与水分利用特性比较[J].北方园艺,2017,41(02):7.[doi:10.11937/bfyy.201702002]
HA Xiaoli,HAN Zhanjiang,LIANG Jiye.Comparasion of Photosynthetic and Water Use Characteristics of Different Varieties (Lines) in Prunus humilis[J].Northern Horticulture,2017,41(09):7.[doi:10.11937/bfyy.201702002]
[10]韩轩轩,王鹏飞,穆霄鹏,等.地布覆盖下不同垄型对欧李园土壤温湿度与果实品质的影响[J].北方园艺,2018,42(20):25.[doi:10.11937/bfyy.20180715]
HAN Xuanxuan,WANG Pengfei,MU Xiaopeng,et al.Effects of Different Ridge Shapes With Ground Cover on Soil Temperature,Humidity and Fruit Quality of Chinese Dwarf Cherry[J].Northern Horticulture,2018,42(09):25.[doi:10.11937/bfyy.20180715]
备注/Memo
第一作者简介：张晓艳（1986-），女，博士研究生，研究方向为果树栽培生理。E-mail：1002298685@qq.com.责任作者：李晓艳（1979-），女，博士，讲师，现主要从事干寒地区果树逆境生理和分子机制等研究工作。E-mail:lixiaoyan684@126.com.基金项目：内蒙古自治区自然科学基金资助项目（2020MS03083）；内蒙古农业大学高层次人才引进科研启动资助项目（NDYB2016-16）；内蒙古自治区自然科学基金资助项目（2018MS03052）；内蒙古自治区自然科学基金资助项目（2019MS03072）。收稿日期：2020-06-02