[1]任镘蓉,全英杰,岳圆圆,等.菊花[STBX]ClERF1[STBZ]基因的克隆与表达分析[J].北方园艺,2021,(01):66-72.[doi:10.11937/bfyy.20200582]
　REN Manrong,QUAN Yingjie,YUE Yuanyuan,et al.Cloning and Expression Analysis of ClERF1 Gene in Chrysanthemum[J].Northern Horticulture,2021,(01):66-72.[doi:10.11937/bfyy.20200582]
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菊花[STBX]ClERF1[STBZ]基因的克隆与表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年01 页码: 66-72 栏目: 出版日期: 2021-01-15
Title:
Cloning and Expression Analysis of ClERF1 Gene in Chrysanthemum
作者:
任镘蓉; 全英杰; 岳圆圆; 杨文婷; 何子涵; 高日
（延边大学 农学院，吉林 延吉 133002）
Author(s):
REN Manrong; QUAN Yingjie; YUE Yuanyuan; YANG Wenting; HE Zihan; GAO Ri
(College of Agriculture,Yanbian University,Yanji,Jilin 133002)
关键词:
菊花; AP2/ERF; 基因克隆; 启动子元件
Keywords:
chrysanthemum; AP2/ERF; gene cloning; promoter element
DOI:
10.11937/bfyy.20200582
文献标志码:
A
摘要:
以甘菊叶片为试验材料，采用RT-PCR和Tail-PCR以及生物信息学的方法，研究了甘菊ClERF1及其启动子序列特征、表达模式，以及甘菊ClERF1在低温胁迫下的响应。以期为进一步研究甘菊ClERF1功能提供参考依据。结果表明：ClERF1基因序列全长1 242 bp，最大开放阅读框（ORF）618 bp，可编码206个氨基酸。经理化性质分析，ClERF1分子量23 631.35 Da，理论等电点5.19，不稳定系数53.84，脂肪指数62.04，平均亲水性-0.786，亚细胞定位在细胞核内。系统进化树表明，ClERF1与拟南芥At3g23240亲缘关系最近，属于ERF亚家族。qRT-PCR分析表明ClERF1在叶片中表达最高，其次在茎段中。该研究克隆了ClERF1启动子序列1 534 bp，主要包括低温反应和乙烯响应元件、生长素和茉莉酸甲酯反应元件等。甘菊低温处理幼苗ClERF1表达量显著高于未低温处理的幼苗，其中5 ℃时ClERF1相对表达量最高。
Abstract:
The leaves of chamomile were used as the test material,RT-PCR,Tail-PCR and bioinformatics methods were used to study the characteristics and expression patterns of the promoter sequences of ClERF1 and its response to low temperature stress.In order to provide a theoretical basis for further study on the function of chamomile ClERF1.The results showed that ClERF1 gene sequence was 1 242 bp,with a maximum open reading frame (ORF) of 618 bp,encoding 206 amino acids.Manager property analysis showed that ClERF1 molecular weight 23 631.35 Da,theoretical pI 5.19,instability index 53.84,aliphatic index 62.04,grand average of hydrophilicity -0.786,and subcellular location in the nucleus.Phylogenetic tree showed that ClERF1 was closely related to arabidopsis At3g23240 and belonged to the ERF subfamily.qRT-PCR analysis showed that ClERF1 expressed the highest expression in leaves,followed by in stem segments.ClERF1 promoter sequence of 1 534 bp was cloned,which mainly included low temperature reaction and ethylene response element,auxin and methyl jasmonate reaction element.The expression level of ClERF1 in chamomile seedlings treated at different low temperatures was significantly higher than that in the seedlings not treated at low temperatures,and the relative expression level of ClERF1 was the highest at 5 ℃.

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备注/Memo

第一作者简介：任镘蓉（1995-），女，硕士研究生，研究方向为菊花遗传育种。E-mail：3116610846@qq.com.责任作者：高日（1982-），男，博士，讲师，现主要从事菊花遗传育种等研究工作。E-mail：gaori@ybu.edu.cn.基金项目：国家自然科学基金地区科学基金资助项目（32060696）;吉林省“十三五”科学技术资助项目（JJKH20180902KJ）；延边大学青年基金资助项目（延大科合字（2017）第21号）；延边大学博士科研启动基金资助项目（602018027）；农业农村部景观设计重点实验室开放课题资助项目（KF201902）。收稿日期：2020-02-19

更新日期/Last Update: 2021-04-08