[1]张雁凌,秦宇婷,赵文婷,等.超声波-微波协同法提取分蘖葱头有机硫化合物的工艺优化及其组分分析[J].北方园艺,2021,(04):93-100.[doi:10.11937/bfyy.20200566]
　ZHANG Yanling,QIN Yuting,ZHAO Wenting,et al.Optimization and Component Analysis of Ultrasonic-microwave Synergistic Extraction of Organic Sulfur-containing Compounds From Allium cepa L.var.agrogatum Don[J].Northern Horticulture,2021,(04):93-100.[doi:10.11937/bfyy.20200566]

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超声波-微波协同法提取分蘖葱头有机硫化合物的工艺优化及其组分分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年04 页码: 93-100 栏目: 出版日期: 2021-02-28

Title:: Optimization and Component Analysis of Ultrasonic-microwave Synergistic Extraction of Organic Sulfur-containing Compounds From Allium cepa L.var.agrogatum Don

作者:: 张雁凌1; 秦宇婷2; 赵文婷2; 3; 刘鸿铖2; 4; 刘婷婷2; 5; （1.吉林工程技术师范学院，吉林长春 130052；2.吉林农业大学食品科学与工程学院，吉林长春 130118；3.吉林省粮食精深加工与副产物高效利用技术创新重点实验室，吉林长春 130118；4.吉林省粮食精深加工与高效利用工程研究中心，吉林长春 130118；5.农业农村部食用菌加工技术集成科研基地，吉林长春 130118）

Author(s):: ZHANG Yanling1; QIN Yuting2; ZHAO Wenting2; 3; LIU Hongcheng2; 4; LIU Tingting2; 5; (1.Jilin Engineering Normal University,Changchun,Jilin 130052;2.College of Food Science and Engineering,Jilin Agricultural University,Changchun,Jilin 130118;3.Key Laboratory of Technological Innovations for Grain Deep-processing and High-effeciency Utilization of By-products of Jilin Province,Changchun,Jilin 130118;4.Engineering Research Center of Grain Deep-processing and High-effeciency Utilization of Jilin Province,Changchun,Jilin 130118;5...

关键词:: 超声波-微波协同法; 分蘖葱头; 有机硫化合物; 气相色谱-质谱联用

Keywords:: ultrasonic-microwave synergistic extraction; Allium cepa L.var.agrogatum Don; organic sulfur-containing compounds; gas chromatography-mass spectrometry

DOI:: 10.11937/bfyy.20200566

文献标志码:: A

摘要:: 以分蘖葱头为试材，采用超声波-微波协同法提取有机硫化合物，进行分蘖葱头有机硫化合物工艺优化试验，并利用气相色谱-质谱联用仪对提取物组分进行分离鉴定，以期为进一步研究和开发分蘖葱头功能组分提供参考依据。结果表明：最佳提取工艺为微波温度55 ℃、料液比1∶8 g?mL-1、协同时间6 min、搅拌转速300 r?min-1、超声波功率500 W，该条件下有机硫化合物得率为0.277 1%±0.002 3%。该条件得到15种有机硫化合物，含量较高的是噻吩类39.067 8%±0.004 3%，其次是三硫化合物14.157 0%±0.007 1%和硫醚类7.387 2%±0.005 2%，其中含量相对较高的有机硫化合物为2,4-二甲基噻吩、3,5-二乙基-1,2,4-三硫杂环戊烷、二烯丙基二硫醚。

Abstract:: Taking Allium cepa L.var.agrogatum Don as test material,organic sulfur-containing compounds were extracted by ultrasonic-microwave synergistic extraction.The process optimization test of organic sulfur compounds were carried out,and the components of extraction were separated and identified by gas chromatography-mass spectrometry in order to provide a reference for further research and development of functional components of Allium cepa L.var.agrogatum Don.The results showed that the optimal extraction process was the microwave temperature of 55 ℃,the ratio of material-liquid of 1∶8 g?mL-1,the synergistic time of 6 minutes,the stirring speed of 300 r?min-1,and the ultrasonic power of 500 W.The yield of the organic sulfur-containing compounds obtained under the above conditions was 0.277 1%±0.002 3%.Under this condition,fifteen kinds of organic sulfur-containing compounds were obtained.The higher content was thiophenes 39.067 8%±0.004 3%,followed by trisulfide compounds 14.157 0%±0.007 1% and thioethers 7.387 2%±0.005 2%.Among them，the relatively high content of organic sulfur-containing compounds was 2,4-dimethyl-thiophene,3,5-diethyl-1,2,4-trithiolane,diallyl disulphide.

参考文献/References:

[1]刘蒙佳,周强,张宝善,等.分蘖葱头油脂微胶囊的研制[J].食品工业,2017,38(1):81-84.[2]刘超,高炯杰.超声波辅助法提取毛葱黄酮的工艺研究[J].食品研究与开发,2015,36(18):50-54.[3]GUNTHER W H H.Garlic and other alliums-the lore and the science[J].Sulfur Reports,2010,34(1-2):208-208.[4]NICASTRO H L,ROSS S A,MILNER J A.Garlic and onions:Their cancer prevention properties[J].Cancer Prevention Research,2015,8(3):181-189.[5]黄晴,吴忠坤,吴中琴,等.葱属类植物中有机硫化物的抗氧化性研究进展[J].食品研究与开发,2018,39(1):214-220.[6]付学军,金海珠,黎乃维,等.系列洋葱深加工研究及其产业化前景探析[J].农产品加工(学刊),2006(6):4-7.[7]MAIDMENT D C J,DEMBNY Z,WATTS D I.The anti-bacterial activity of 12 Alliums against Escherichia coli[J].Nutrition & Food Science,2001,31(5):238-241.[8]MARTA C,CORZO N,VILLAMIEL M.Biological properties of onions and garlic[J].Trends in Food Science & Technology,2007,18(12):609-625.[9]YANAGITA T,HAN S Y,WANG Y M,et al.Cycloalliin,a cyclic sulfur imino acid，reduces serum triacylglycerol in rats[J].Nutrition,2003,19(2):140-143.[10]刘超.分蘖葱头槲皮素对高血脂小鼠血脂影响[J].食品研究与开发,2015,36(17):19-23.[11]RAMIREZ D A,LOCATELLI D A,TORRES-PALAZZOLO C A,et al.Development of garlic bioactive compounds analytical methodology based on liquid phase microextraction using response surface design.Implications for dual analysis:Cooked and biological fluids samples[J].Food Chemistry,2017,215(15):493-500.[12]孙淑爱,曹晶,王永明,等.大蒜油蒸馏法提取条件的探讨[J].实用医药杂志,2004,21(4):347-348.[13]MARTIN-LAGOS R,SERRANO M,RUIZ-LOPEZ M.Comparative study by gas chromatography-mass spectrometry of methods for the extraction of sulfur compounds in Allium cepa[J].Food Chemistry,1992,44(4):305-308.[14]KREST I,GLODEK J,KEUSGEN M.Cysteine sulfoxides and alliinase activity of some allium species[J].Journal of Agricultural and Food Chemistry,2000,48(8):3753-3760.[15]彭光华,韩月峰,马荣池,等.大蒜有机有机硫化合物提取条件的优化及其成分的分析[J].食品科学,2008,29(7):226-230.[16]孙颖,陈怡颖,丁琦,等.小根蒜挥发性风味成分分析[J].食品科学,2015,36(16):117-121.[17]帕尔哈提?克依木,周晓龙,刘河疆.气质联用法测定甜瓜中代森锌[J].食品研究与开发,2017,38(17):134-138.[18]刘婷婷,秦宇婷,吴玉莹,等.溶剂法提取分蘖葱头有机硫化合物及其GC-MS分析[J].食品科学,2017,38(12):151-156.[19]徐艳阳,胡晓欢,王乃茹,等.大葱中葱辣素的微波辅助提取工艺优化[J].食品研究与开发,2014,35(13):27-29.[20]王希丽,张建丽,何洪巨.GC-MS法测定大蒜中的挥发性物质[J].分析测试学报,2004(S1):107-109.[21]CHENG L,LUO J,LI P,et al.Microbial diversity and flavor formation in onion fermentation.[J].Food & Function,2014,5(9):2338-2347.[22]MIETHING H.Allicin and oil in garlic bulbs-HPLC quantitative determination[J].Dtsch Apoth Ztg,1985,125:2049-2150.

备注/Memo

第一作者简介：张雁凌（1974-），女，硕士，讲师，现主要从事农产品贮藏与加工等研究工作。E-mail:617546087@qq.com.责任作者：刘婷婷（1984-），女，博士，副教授，现主要从事谷物食品科学与副产物高值化利用等研究工作。E-mail:ltt1984@163.com.基金项目：吉林农业大学人才计划资助项目（20160509）。收稿日期：2020-02-18

更新日期/Last Update: 2021-06-07