YU Han,ZHANG Jingyi,HU Yiqing,et al.Study on Physiological Responses of Flammulina velutipes Under Stress of Mn2+ and Pb2+[J].Northern Horticulture,2020,44(18):132-136.[doi:10.11937/bfyy.20194760]
锰和铅胁迫下金针菇生理反应研究
- Title:
- Study on Physiological Responses of Flammulina velutipes Under Stress of Mn2+ and Pb2+
- Keywords:
- heavy metals; poisoning; Flammulina velutipes
- 文献标志码:
- A
- 摘要:
- 以金针菇T011菌种的菌根为试材,采用不同浓度Mn2+和Pb2+溶液进行单一胁迫处理,探究不同重金属离子对金针菇的生长发育过程中可溶性糖、可溶性蛋白质含量和过氧化氢酶活性的影响,以期为进一步开展金针菇大规模生产和环境污染治理提供参考依据。结果表明:2种重金属离子在达到一定浓度时均能抑制金针菇生长和减少可溶性糖含量。其中,Mn2+溶液对金针菇菌根的胁迫影响较为显著,而Pb2+胁迫提升了金针菇可溶性蛋白质的积累,但对可溶性糖含量积累没有明显作用。此外,2种重金属离子胁迫均促进了金针菇内部过氧化氢酶活性,且均在500 mg?L-1时,过氧化氢酶活性达到最大。因此,Pb2+和Mn2+对金针菇生长发育的影响是复杂的过程,不能从单一的生理指标变化分析趋势。
- Abstract:
- Based on the roots of Flammulina velutipes T011 treated by different concentrations of Mn2+ and Pb2+ solutions,the effects of different heavy metal ions on catalase enzyme activity,soluble protein and soluble sugar contents in the growth and development of mushroom were investigated.In order to provide theoretical basis for the further development of large-scale production of mushroom and environmental pollution control.The results showed that both heavy metal ions could inhibit the growth and reduce the soluble sugar content of the mushroom.Specifically,the growth of mycorrhiza was sensitive to Mn2+,and the soluble protein content increased with the increasing of Pb2+ concentration,while there was no significant effect on the soluble sugar content.In addition,both heavy metal ion stresses could promote the accumulation of catalase enzyme activity in the mushroom,which reached the maximum at a concentration of 500 mg?L-1.In summary,the change of physiological indicators alone did not fully reveal the mechanism of Pb2+ and Mn2+ on Flammulina velutipes.
参考文献/References:
[1]李鹏辉,邹晓燕,楼婕,等.不同季节垃圾填埋场周围重金属污染特征及评价[J].环境化学,2018,37(1):41-50.[2]吴耀文,杨灿鑫,李欣航,等.锰胁迫对野胡萝卜(Daucus carota)幼苗生长及理化特性的影响[J].北方园艺,2019(10):23-30.[3]许文博,邵新庆,王宇通,等.锰对植物的生理作用及锰中毒的研究进展[J].草原与草坪,2011,31(3):5-14.[4]冯三畏.锰的神经毒作用研究进展[C].西安:中华预防医学会石油系统分会第七届预防医学学术交流会,2011.[5]EHSAN N,NAWAZ R,AHMAD S,et al.Use of ornamental plant ‘Vinca’(Vinca rose L.) for remediation of lead contaminated soil[J].Biodiv Environ Sci,2016,8(3):46-54.[6]郭健,姚云,赵小旭,等.粮食中重金属铅离子、镉离子的污染现状及对人体的危害[J].粮食科技与经济,2018,43(3):33-35,85.[7]高纯阳.金针菇(Flammulina velutipes)休闲食品的加工研究[D].南京:南京农业大学,2015.[8]周建军,周桔,冯仁国.我国土壤重金属污染现状及治理战略[J].中国科学院院刊,2014,29(3):315-320,350,272.[9]刘铮.土壤与植物中锰的研究进展[J].土壤学进展,1991(6):1-10,22.[10]黄晓辉,王春晖,徐宁,等.金针菇、杏鲍菇菌丝对四种重金属耐受和富集特性的研究[J].北方园艺,2019(3):143-150.[11]唐蛟,马学军,霍继鹏,等.重金属Cr、Hg胁迫对油白菜种子萌发及幼苗生长的影响[J].河南科技学院学报(自然科学版),2019,47(2):8-13.[12]李文砚,韦持章,孔方南,等.蒽酮法测定木奶果果实中可溶性糖含量的研究[J].中国园艺文摘,2015,31(12):7-8,28.[13]焦洁.考马斯亮蓝G-250染色法测定苜蓿中可溶性蛋白含量[J].农业工程技术,2016,36(17):33-34.[14]王春台,徐同,刘学群.紫外分光光度法测定过氧化氢酶的活性[J].华中农业大学学报,1987(1):77-81. [15]王海青.铅锌汞三种重金属对莜麦生理毒性的研究[D].太原:山西大学,2014.[16]齐文靖,于晗,张佳慧,等.不同重金属胁迫对绿豆种子萌发和幼苗部分生理指标的影响[J].北方园艺,2018(21):1-5.[17]王妙媛.Pb2+、Cd2+胁迫对红花檵木生长及生理生化特性的影响[D].长沙:湖南农业大学,2011.[18]文珂,刘文胜.锰胁迫对青葙种子萌发和幼苗生理的影响[J].广东农业科学,2017,44(6):78-83.[19]郝雅宾,李凤玉,刘海山.锰、铁胁迫对商陆和高羊茅可溶性蛋白的影响[J].福建师范大学报(自然科学版),2013,29(3):97-103.[20]何晓良,蔡卓,邱霞琳,等.铅胁迫对狮子草可溶性蛋白含量的影响[J].广西大学学报(自然科学版),2013,38(3):550-555.[21]李艳艳.双孢蘑菇对重金属铅、镉富集规律的初步研究[D].武汉:华中农业大学,2011.
相似文献/References:
[1]王 强.重金属对苔藓植物影响的研究进展[J].北方园艺,2014,38(10):169.
WANG Qiang.Advance in the Effects of Heavy Metals on Bryophytes[J].Northern Horticulture,2014,38(18):169.
[2]陈晓云,张亚文,詹德江,等.辽宁海城设施蔬菜土壤重金属污染评价研究[J].北方园艺,2014,38(01):165.
CHEN Xiao-yun,ZHANG Ya-wen,ZHAN De-jiang,et al.Study on Evaluation of Soil Heavy Metal Pollution of Greenhouse Vegetable in North China[J].Northern Horticulture,2014,38(18):165.
[3]贺小琴,张永清.离石区蔬菜大棚土壤重金属污染现状评价[J].北方园艺,2014,38(04):155.
HE Xiao-qin,ZHANG Yong-qing.Assessment of Heavy Metal Pollution in Vegetable Greenhouse in Lishi District[J].Northern Horticulture,2014,38(18):155.
[4]吴大付,朱东海,吴海卿,等.重金属污染土壤的植物修复对“环保育种”的呼唤[J].北方园艺,2013,37(10):190.
WU Da-fu,ZHU Dong-hai,WU Hai-qing,et al.Requirement of ‘Environmental Breeding’ for Phytoremediation of Heavy Metal Contaminated Soils[J].Northern Horticulture,2013,37(18):190.
[5]梁巧玲,杨晖,赵鹂.丽水市郊蔬菜重金属含量及健康风险分析[J].北方园艺,2013,37(12):21.
LIANG Qiao-ling,YANG Hui,ZHAO Li.Analysis of Heavy Metal Contents in Vegetables of Lishui Suburb and the Health Hazard[J].Northern Horticulture,2013,37(18):21.
[6]张满满,肖 昕,谭 敏.徐州北郊土壤重金属污染评价研究[J].北方园艺,2013,37(06):177.
ZHANG Man-man,XIAO Xin,TAN Min.Study on the Soil Heavy Metals Pollution Evaluation in the Northern Suburb of Xuzhou City[J].Northern Horticulture,2013,37(18):177.
[7]关梦茜,董然.重金属污染土壤的花卉植物修复研究进展[J].北方园艺,2013,37(21):187.
GUAN Meng-xi,DONG Ran.Research Progress on the Flowers Remediation in Heavy Metal Pollution Soil[J].Northern Horticulture,2013,37(18):187.
[8]马文哲,王文光,吴春霞,等.杨凌示范区蔬菜中重金属污染分析与评价[J].北方园艺,2012,36(17):46.
MA Wen-zhe,WANG Wen-guang,WU Chun-xia,et al.Analysis and Evaluation on Heavy Metal Pollution of Vegetables in Yangling Demonstration Zone[J].Northern Horticulture,2012,36(18):46.
[9]李庚飞.几种菊科植物对矿区土壤重金属的吸收研究[J].北方园艺,2012,36(06):72.
LI Geng-fei.Study on the Contents of Heavy Metals in Several Compositae Plants in Gold Area[J].Northern Horticulture,2012,36(18):72.
[10]任伟,张思冲,王春光,等.哈尔滨交通干道两侧土壤重金属潜在生态危害评价[J].北方园艺,2012,36(06):141.
REN Wei,ZHANG Si-chong,WANG Chun guang,et al.The Potential Ecological Risk Assessment of Heavy Metals on Both Sides of the Harbin City Road[J].Northern Horticulture,2012,36(18):141.
备注/Memo
第一作者简介:于晗(1997-),女,硕士研究生,研究方向为功能分子生物学。E-mail:y114731@163.com.责任作者:倪秀珍(1967-),女,博士,教授,硕士生导师,现主要从事功能分子生物学等研究工作。E-mail:2035084581@qq.com.基金项目:国家自然科学基金面上资助项目(31870486);吉林省教育厅“十三五”科学技术重点资助项目(JJKH20170657KJ);吉林省科技厅自然科学基金资助项目(20200201133JC)。收稿日期:2019-12-17