ZONG Shubin,WANG Yongping,TIAN Xingjun,et al.Comparison on Multiplication of Vaccinium corymbosum Plantlets in Several New Type Temporary Immersion Bioreactors System[J].Northern Horticulture,2020,44(13):47-51.[doi:10.11937/bfyy.20194192]
种植密度和播种期对节瓜生育及产量的影响
- Title:
- Comparison on Multiplication of Vaccinium corymbosum Plantlets in Several New Type Temporary Immersion Bioreactors System
- Keywords:
- bioreactor; multiplication culuture; Vaccinium corymbosum
- 文献标志码:
- A
- 摘要:
- 以“桂优三号”毛节瓜为试材，研究不同种植密度和播种期对池州地区节瓜生育进程及产量和产量构成因素的影响，以期探索“桂优三号”毛节瓜在池州地区适宜的播种期及种植密度。结果表明：池州地区3月15日播种，667 m2种植密度为1 800株，667 m2产量可达4 458.5 kg，与其它处理间存在极显著差异。因此，池州地区春播毛节瓜应在3月中旬播种，667 m2种植密度1 800株。
- Abstract:
- The third generation of blueberry robust rootless tissue culture plantlets were used as the test materials.The multiplication coefficient，growth indexes and physiological indexes of the proliferation plantlets in different bioreactors(swing type,aerosol type,double bottle type) were studied，so as to select the best proliferation culture bioreactor and culture method.The results showed that the multiplication coefficient had been significantly improved in the new bioreactors，and it was 2.0-2.5 times to the control.Comprehensive analysis of multiplication efficiency，the aerosol type better than the swing type better than the double bottle type.The growth indexes of the multiplication seedlings in the new bioreactors were significantly better than those of the control，and the optimal growth indexes was double bottle culture，and the new bioreactor could significantly lower the browning degree of the multiplication seedlings.
参考文献/References:
[1]贺强,吴立仁.蓝莓果实中营养成分的生物学功能[J].北方园艺,2010(24):222-224.[2]BORNSEK S M,ZIBERNA L,POLAK T，et al.Bilberry and blueberry anthocyanins act as powerful intracellular antioxidants in mammalian cells[J].Food Chem，2012,134(4):1878-1884.[3]赵秀玲.蓝莓的成分与保健功能的研究进展[J].中国野生植物资源,2011,30(6):19-23.[4]王姗姗,孙爱东,李淑燕.蓝莓的保健功能及其开发应用[J].中国食物与营养，2010（6):17-20.[5]WU X,RAHAL O，KANG J，et al.In utero and lactational exposure to blueberry via maternal diet promotes mammary epithelial differentiation in prepubescent female rats[J].Nutr Res，2009,29(11)：802-811.[6]DELBO C,MARTINI D,VENDRAME S，et al.Improvement of lymphocyte resistance against H2O2-induced DNA damage in Sprague-Dawley rats after eight weeks of a wild blueberry (Vaccinium angustifolium)-enriched diet[J].Mutat Res-Genet Toxicol Environ Mutagen，2010,703(2)：158-162.[7]LACOMBE A，WU VCH，WHITE J，et al.The antimicrobial properties of the lowbush blueberry (Vaccinium angustifolium) fractional components against foodborne pathogens and the conservation of probiotic Lactobacillus rhamnosus[J].Food Microbiol,2012,30(1)：124-131.[8]KADER F，ROVEL B，GIRARDIN M,et al.Fractionation and identification of the phenolic compounds of Highbush blueberries (Vaccinium corgmbosum L.)[J].Food Chem,1996,55(1):35-40.[9]高美萍,林志城,张驰等.荸荠组培苗在间歇浸没式生物反应器(TIBs)中的高效增殖技术[J].南方农业学报，2016，47(10):1653-1657.[10]赵望锋,王力华.间歇浸没式生物反应器在大规模组织培养中的应用研究[J].安徽农业科学，2007，35(2):317-320，323.[11]MEYER G M，BANASIAK M，NTOYI T T，et al.Sugarcane plants from temporary immersion culture：Acclimating for commercial production[J].Acta Hortic,2009，812：323-327.[12]PEA-RAMREZ Y J，JUREZ-GMEZ J，GMEZ-LPEZ L,et al.Multiple adventitious shoot formation in Spanish red cedar (Cedrela odorata L.) cultured in vitro using juvenile and mature tissues：An improved micropropagation protocol for a highly valuable tropical tree species[J].In Vitro Cell Dev-Pl,2010,46:149-160.[13]SNYMAN S J,MEYER G M,KOCH A C,et al.Applications of in vitro culture systems for commercial sugarcane production and improvement[J].In Vitro Cell Dev-Pl,2011a,47：234-249.[14]杨柳，秦钢，粟靖等.利用间歇浸没式生物反应器进行香蕉(Musa AAA Cavendish var.Williams B6)组培快繁研究[J].果树学报,2010b,27(6):1010-1013.[15]杨柳，秦钢，杨丽涛，等.利用间歇浸没式生物反应器进行果蔗组培快繁[J].热带作物学报,2010a,31(4):614-620.[16]杨柳，秦钢，杨丽涛，等.利用间歇浸没式生物反应器进行甘蔗组培快繁的研究[J].华南农业大学学报,2011,32(1):37-41.[17]许亚良,张家明.一种高效大规模组培方法:间歇浸没培养法[J].植物生理学报，2013,49(4):392-399.[18]张转转,石琨,于镇榕，等.基于植物微扩繁器的草莓快繁技术的研究[J].中国农学通报,2014,30(16):216-220.[19]石琨，郑彩霞.美国红栌液体快繁体系的建立[J].植物生理学报,2015,51(4):553-558.[20]高美萍,林志城,张驰,等.荸荠组培苗在间歇浸没式生物反应器(TIBs)中的高效增殖技术[J].南方农业学报,2016,47(10):1653-1657.[21]高俊凤.植物生理学实验指导[Ｍ].北京:高等教育出版社，2006.[22]张宪政,陈凤玉,王荣富.植物生理学实验技术[Ｍ]．沈阳:辽宁科学技术出版社,１９９４．[23]READ S M，NORTHEOTE D H.Minimization of yariation in the response to different protein of the coomassic blue Gdye-blinding for protein[J].Analytical Biochemistry,1981,116:53-64.[24]林加涵,魏文铃,彭宣宪.现代生物学实验[M].北京:高等教育出版社,2001.[25]TAN W，LIU J，DAI T，et al.Alterations in photosynthesis and antioxidant enzyme activity in winter wheat subjected to post-anthesis water-logging[J].Photos Ynthetica,2008,46:21-27.[26]李雪琴,张雪娇,陈洁，等.小麦籽粒多酚氧化酶测定方法的研究[J].粮食工程，2012（6）：71-73.[27]CHEN S J，ZHONG G Y，XU J H.Coptis study on soluble sugar and amino acid contents during mate rnityleave[J].China Journal of Chinese Materia Medica,2005,30(17):13-26.[28]JIANG D，YU Z W，LI Y K.Winter wheat leaf stem relationship between soluble sugar contents and starchaccumulation[J].Journal of Triticeae Crops，2001,21(3):38.[29]LOOMIS W D,BATTAILE J.Plant phenolic compouds and the isolation of plant enzymes[J].Phytochemistry,1966,5(3):423-438.[30]王小燕.黄连木雌雄株间生物学特性的比较研究[D].洛阳:河南科技大学，2013.
相似文献/References:
[1]孔小平.高寒地区日光温室节瓜引种栽培技术[J].北方园艺,2013,37(12):37.
KONG Xiao-ping.Introduction and Cultivation Technique of Benincasa hispida in Solar Greenhouse in Alpine Region[J].Northern Horticulture,2013,37(13):37.
[2]孙继,王晓艳,唐筱春,等.播种期对温州春榨菜生物学特性及产量的影响[J].北方园艺,2012,36(03):38.
SUN Ji,WANG Xiao-yan,TANG Xiao-chun,et al.Effect of Sowing Time on Biological Characteristics and Yield of Spring Tuber Musterd in Wenzhou[J].Northern Horticulture,2012,36(13):38.
[3]刘希财,建德锋.吉林地区药用植物鸡冠花不同播种期露地直播繁殖试验[J].北方园艺,2012,36(14):170.
LIU Xi-cai,JIAN De-feng.Study on the Breeding in Open Field of Medicinal Plant Celosia cristata in Different Sowing Time in Jilin Area[J].Northern Horticulture,2012,36(13):170.
[4]刘政国,王鹏,陈勇,等.小冬瓜(节瓜)吊蔓贮藏方式对果实品质及耐贮性的影响[J].北方园艺,2014,38(24):123.
LIU Zheng-guo,WANG Peng,CHEN Yong,et al.Effect of Storage by Hanging on the Stems on Storage Characteristics and Quality of Chieh-qua Fruit[J].Northern Horticulture,2014,38(13):123.
[5]黄成秋,刘政国,覃海平,等.栽植密度与支架方式对节瓜产量的影响[J].北方园艺,2016,40(05):46.[doi:10.11937/bfyy.201605013]
HUANG Chengqiu,LIU Zhengguo,QIN Haiping,et al.Effect of Planting Density and Humping Support on Yield of Chieh-qua[J].Northern Horticulture,2016,40(13):46.[doi:10.11937/bfyy.201605013]
[6]黎 炎,李 文 嘉,王 益 奎,等.节 瓜 果 皮 颜 色 遗 传 规 律 的 研 究[J].北方园艺,2007,31(10):0.[doi:10.11937/bfyy.200701006]
LI Yan,LI Wen - jia,WANG Yi - kiu,et al.Study on Inheritance of Fruit Colour in Chieh- qua[J].Northern Horticulture,2007,31(13):0.[doi:10.11937/bfyy.200701006]
[7]刘政国,秦荣耀.节瓜种子休眠机理的初步研究[J].北方园艺,2006,30(02):0.[doi:10.11937/bfyy.200602009]
LIU Zhengguo,QIN Rongyao.Studies on Dormancy Mechanism in Chieh-qua[J].Northern Horticulture,2006,30(13):0.[doi:10.11937/bfyy.200602009]
[8]高晶霞,陈书霞,程智慧,等.播期及整枝方式对设施厚皮甜瓜生长发育和产量的影响[J].北方园艺,2010,34(03):0.[doi:10.11937/bfyy.201003074]
GAO Jing-xia,CHEN Shu-xia,CHENG Zhi-hui,et al.Effect of Sowing Date and Trainning Types on Plant Growth and Yield of Muskmelon in the Protected Structure[J].Northern Horticulture,2010,34(13):0.[doi:10.11937/bfyy.201003074]
[9]朱冬冬,刘政国,鲍遵宇,等.秋季不同播种期对节瓜生育特性及产量的影响[J].北方园艺,2016,40(22):11.[doi:10.11937/bfyy.201622003]
ZHU Dongdong,LIU Zhengguo,BAO Zunyu,et al.Effects of Autumnal Different Sowing Dates on Production and Reproductive Characteristics on Chieh-qua[J].Northern Horticulture,2016,40(13):11.[doi:10.11937/bfyy.201622003]
备注/Memo
第一作者简介：方宇鹏（1987-），女，安徽池州人，硕士，讲师，研究方向为植物栽培。E-mail:583122383@qq.com.基金项目：安徽省高校优秀青年人才支持计划一般资助项目（gxyqZD2019224）；安徽省质量工程项目-园艺专业教学团队资助项目（2018jxtd055）；池州职业技术学院院级质量工程“园林植物与观赏园艺”教学团队资助项目（2017jxtd03）。收稿日期：2020-03-09